ISSN:
0368-1467
Schlagwort(e):
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
A method has been developed for the recovery of enterotoxin from bacterial culture supernatants of staphylococcus strain S-6 by use of the ion-exchange resin Amberlite XE-64 (IRC-50). The enterotoxin from 200 ml of bacterial culture supernatant diluted with one volume of water and adjusted to pH 6 can be removed with 1 g of resin pretreated with 0·02 M sodium phosphate, pH 6·2. The enterotoxin is recovered from the resin with 0·2 M sodium phosphate, pH 6·2, with subsequent precipitation with EtOH at -10°C, dialysis, and freeze drying. Partially purified preparations containing 1 emetic dose per 50μg were obtained.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jbmte.390030106
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