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  • Articles  (1,576)
  • Medicine  (1,017)
  • Geosciences  (560)
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  • Articles  (1,576)
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  • 1
    Electronic Resource
    Electronic Resource
    Diphenylcarbazide acetonitrile solvate (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. o312-o314 
    Details
    ISSN: 1600-5368
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: In the crystal lattice of the title compound, 1,5-diphenylcarbonohydrazide acetonitrile solvate, C13H14N4O·C2H3N, the diphenylcarbazide molecules create a network structure through hydrogen bonds. The crystal structure is stabilized by N—H...N and N—H...O hydrogen bonds. The FT–IR spectra clearly show the presence of acetonitrile molecules in the crystal lattice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S160053680100397X
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of hpuAB, the haemoglobin–haptoglobin-utilization operon of Neisseria meningitidis (1997)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 23 (1997), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We previously identified HpuB, an 85 kDa Fe-repressible protein required for utilization of Fe from, and binding to, haemoglobin and the haemoglobin–haptoglobin complex. The gene for hpuB was cloned from Neisseria meningitidis strain DNM2 and the predicted amino acid sequence indicates that HpuB is an outer membrane receptor belonging to the TonB family of high-affinity transport proteins. A second open reading frame, predicted to encode a 34.8 kDa lipoprotein, was discovered 5′ to hpuB, and was designated hpuA. HpuA was identified in a total-membrane-protein preparation by construction of a mutant lacking HpuA. Acylation of HpuA was confirmed by [3H]-palmitic acid labelling of meningococci. Consensus promoter sequences were not apparent 5′ to hpuB. The hpuA insertion mutation exerted a polar effect, abolishing expression of hpuB, suggesting that hpuA and hpuB are co-transcribed. The 3.5 kb polycistronic hpuAB mRNA was identified and shown to be transcriptionally repressed by iron. The transcriptional start site was identified 33 nucleotides 5′ to the hpuA translational start site, appropriately positioned around consensus promoter and ferric uptake regulator (Fur)-box sequences. The structure of this operon suggests that HpuA–HpuB is a two-component receptor analogous to the bipartite transferrin receptor TbpB–TbpA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1997.2501619.x
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of innate and adaptive immune responses by MAP kinase phosphatase 5 (2004)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 430 (2004), S. 793-797 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Mitogen-activated protein (MAP) kinases are essential regulators in immune responses, and their activities are modulated by kinases and phosphatases. MAP kinase phosphatase (MKP) is a family of dual-specificity phosphatases whose function is evolutionarily conserved. A number of mammalian MKPs ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature02764
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  • 4
    Electronic Resource
    Electronic Resource
    Surface textures of turbidite sand grains, Laurentian Fan and Sohm Abyssal Plain (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Sedimentology 29 (1982), S. 0 
    Details
    ISSN: 1365-3091
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: Surface textures of quartz grains have been examined from five samples from the Laurentian Fan and Sohm Abyssal Plain, representing varied transport distances and power of the depositing turbidity current. The grains retain their primary irregular shape derived from glacial erosion, and glacial surface textures are preserved in dish-shaped depressions. These features have been superimposed by a slight rounding of edges and an abundance of collision-induced markings, particularly mechanical V-forms. The most intense current modification of this sort occurs in mid-Wisconsinan or earlier sands that have been transported over 1000 km to the distal Sohm Abyssal Plain by turbidity currents. Collision textures probably develop during grain flow on the steep continental slope: delicate resedimented shelf foraminifera are preserved in the same turbidites and most have been transported exclusively in suspension.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3091.1982.tb00077.x
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  • 5
    Electronic Resource
    Electronic Resource
    A conserved region in the σ54-dependent activator DctD is involved in both binding to RNA polymerase and coupling ATP hydrolysis to activation (1997)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 26 (1997), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Rhizobium melioti DctD activates transcription from the dctA promoter by catalysing the isomerization of closed complexes between σ54-RNA polymerase holoenzyme and the promoter to open complexes. DctD must make productive contact with σ54-holoenzyme and hydrolyse ATP to catalyse this isomerization. To define further the activation process, we sought to isolate mutants of DctD that had reduced affinities for σ54-holoenzyme. Mutagenesis was confined to the well-conserved C3 region of the protein, which is required for coupling ATP hydrolysis to open complex formation in σ54-dependent activators. Mutant forms of DctD that failed to activate transcription and had substitutions in the C-terminal half of the C3 region were efficiently cross-linked to σ54 and the β-subunit of RNA polymerase, suggesting that they bound normally to σ54-holoenzyme. In contrast, some mutant forms of DctD with amino acid substitutions in the N-terminal half of the C3 region had reduced affinities for σ54 and the β-subunit in the cross-linking assay. These data suggest that the N-terminal half of the C3 region of DctD contains a site that may contact σ54-holoenzyme during open complex formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1997.5851955.x
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  • 6
    Electronic Resource
    Electronic Resource
    Local structures of nanocrystalline GaN studied by XAFS (2001)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 8 (2001), S. 830-832 
    Details
    ISSN: 1600-5775
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: X-ray absorption fine structure (XAFS) was used to investigate the local structures around Ga atoms in the hexagonal nanocrystalline and crystalline GaN under 78 and 300 K. For the first nearest neighbor coordination shell of Ga-N, the average bond length R (0.194 nm), coordination number N (4.0), thermal disorder σT (0.0052 nm) and static disorder σS (0.0007 nm) are nearly independent of the measured temperature and crystalline state. This indicates that the Ga-N covalent bond is much stronger, and the 4 nitrogen atoms in first nearest neighbor around Ga atoms keep the tetrahedral structure (Td). For the second nearest neighbor coordination shell of Ga-Ga, their bond lengths are about 0.318 nm. However, the σS (0.0057 nm) of nanocrystalline GaN is 0.0047 nm larger than that of crystalline GaN (0.001nm), and the σT of nanocrystalline is 0.0053 nm and 0.0085 nm at the temperature of 78 and 300 K, respectively. The result indicates that the difference of local structure around Ga atoms between nanocrystalline and crystalline GaN occurs mainly at the Ga-Ga second nearest-neighbor coordination shell. The reason is explained as the local lattice distortion and unsaturated surface atoms existing in nanocrystalline GaN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0909049501000103
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  • 7
    Electronic Resource
    Electronic Resource
    (2-Methylquinolin-8-olato)iron(III) and -copper(II) complexes (2001)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 714-716 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The crystal structures of tris(2-methylquinolin-8-olato-N,O)iron(III), [Fe(C10H8NO)3], (I), and aquabis(2-methylquinolin-8-olato-N,O)copper(II), [Cu(C10H8NO)2(H2O)], (II), have been determined. Compound (I) has a distorted octahedral configuration, in which the central Fe atom is coordinated by three N atoms and three O atoms from three 2-methylquinolin-8-olate ligands. The three Fe—O bond distances are in the range 1.934 (2)–1.947 (2) Å, while the three Fe—N bond distances range from 2.204 (2) to 2.405 (2) Å. In compound (II), the central CuII atom and H2O group lie on the crystallographic twofold axis and the coordination geometry of the CuII atom is close to trigonal bipyramidal, with the three O atoms in the basal plane and the two N atoms in apical positions. The Cu—N bond length is 2.018 (5) Å. The Cu—O bond length in the basal positions is 1.991 (4) Å, while the Cu—O bond length in the apical position is 2.273 (6) Å. There is an intermolecular OW—H...O hydrogen bond which links the molecules into a linear chain along the b axis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270101005534
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  • 8
    Electronic Resource
    Electronic Resource
    Dentinogenesis imperfecta 1 with or without progressive hearing loss is associated with distinct mutations in DSPP (2001)
    [s.l.] : Nature Publishing Group
    Nature genetics 27 (2001), S. 201-204 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Dentinogenesis imperfecta 1 (DGI1, MIM 125490) is an autosomal dominant dental disease characterized by abnormal dentin production and mineralization. The DGI1 locus was recently refined to a 2-Mb interval on 4q21 (ref. 1). Here we study three Chinese families carrying DGI1. We find that the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/84848
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  • 9
    Electronic Resource
    Electronic Resource
    Interaction of KAI1 on tumor cells with DARC on vascular endothelium leads to metastasis suppression (2006)
    [s.l.] : Nature Publishing Group
    Nature medicine 12 (2006), S. 933-938 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] CD82, also known as KAI1, was recently identified as a prostate cancer metastasis suppressor gene on human chromosome 11p1.2 (ref. 1). The product of CD82 is KAI1, a 40- to 75-kDa tetraspanin cell-surface protein also known as the leukocyte cell-surface marker CD82 (refs. 1,2). Downregulation of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm1444
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of Bcl-2 protein in the epiphyseal plate cartilage and trabecular bone of growing rats (1997)
    Springer
    Histochemistry and cell biology 108 (1997), S. 45-55 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The protooncogene protein, Bcl-2, protects cells from apoptosis and ensures their survival in vitro by inhibiting the action of the apoptosis-inducer, Bax. Its expression in proliferative and long-lived cells in vivo also indicates that it protects against cell death. The chondrocytes of the epiphyseal plate cartilage undergo a series of maturation steps and deposit mineral in the cartilage matrix before dying. The possibility that Bcl-2 helps protect chondrocytes until mineral deposition is completed was investigated by determining the distribution of Bcl-2 immunoreactivity in the epiphyseal plate cartilage of growing rats and its subcellular localization, using a specific antibody. The involvement of Bax in the triggering of chondrocyte death was checked by immunocytochemistry. Bcl-2 expression in the osteoblasts and the final result of their evolution, the osteocytes, was also examined in trabecular bone. Bcl-2 immunoreactivity was non-uniformly distributed throughout the epiphyseal cartilage. It was maximal in proliferative chondrocytes, decreased in mature chondrocytes, and low in hypertrophic chondrocytes, whereas there was Bax immunoreactivity in all chondrocytes examined. Immunolabeling was intense in osteoblasts but considerably lower in fully differentiated osteocytes. Bcl-2 immunoreactivity was mainly in the cytoplasm of chondrocytes, osteoblasts, and early osteocytes; the nuclei appeared clear. The subcellular distribution of Bcl-2 immunolabeling in chondrocytes, revealed by gold particles in the electron microscope, showed that gold particles were frequently concentrated in the mitochondria in all the cartilage zones and lay mainly within the organelles, not at their periphery. The endoplasmic reticulum contained moderate immunoreactivity and there were few gold particles in the cytoplasm and nuclei. The number of gold particles decreased in all the subcellular compartments from proliferative to hypertrophic chondrocytes. In contrast, Bax immunoreactivity changed little during chondrocyte terminal evolution, and its subcellular distribution mirrored that of Bcl-2. These immunocytochemical data indicate that Bcl-2 helps maintain chondrocytes and osteoblasts until their terminal maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050145
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