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  • 1
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    In:  J. Struct. Geol., Köln, Elsevier, vol. 27, no. 5, pp. 789-803, pp. B02401, (ISSN: 1340-4202)
    Publication Date: 2005
    Keywords: Tectonics ; Geol. aspects ; Structural geology ; JSG
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  • 2
    Publication Date: 2019-06-27
    Description: High strength abrasive resistant flame resistant flexible impregnated nickel-chromium fabric for space suits
    Keywords: MACHINE ELEMENTS AND PROCESSES
    Type: NASA-CR-65801 , SG-1236FR
    Format: text
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  • 3
    Publication Date: 2017-06-27
    Description: In several fields of cell biology, correlative microscopy is applied to compare the structure of objects at high resolution under the electron microscope with low resolution light microscopy images of the same sample. It is, however, difficult to prepare samples and marker systems that are applicable for both microscopic techniques for the same specimen at the same time. In our studies, we used microbial mats from Cold Seep communities for a simple and rapid correlative microscopy method. The mats consist of bacterial and archaeal microorganisms, coupling reverse methanogenesis to the reduction of sulfate. The reverse methanogenic pathway also generates carbonates that precipitate inside the mat and may be the main reason for the formation of a microbial reef. The mat shows highly differentiated aggregates of various organisms, tightly interconnected by extracellular polysaccharides. In order to investigate the role of EPS as adhesive mucilage for the biofilm and as a precipitation matrix for carbonate minerals, samples were embedded in a hydrophilic resin (Lowicryl K4 M). Sections were suitable for light as well as electron microscopy in combination with lectins, either labeled with a fluorescent marker or with colloidal gold. This allows lectin mapping at low resolution for light microscopy in direct comparison with a highly resolved electron microscopic image.
    Type: Article , PeerReviewed
    Format: text
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  • 4
    Publication Date: 2021-04-25
    Description: During the last few years, the analysis of microbial diversity in various habitats greatly increased our knowledge on the kingdom Archaea. At the same time, we became aware of the multiple ways in which Archaea may interact with each other and with organisms of other kingdoms. The large group of euryarchaeal methanogens and their methane oxidizing relatives, in particular, take part in essential steps of the global methane cycle. Both of these processes, which are in reverse to each other, are partially conducted in a symbiotic interaction with different partners, either ciliates and xylophagous animals or sulfate reducing bacteria. Other symbiotic interactions are mostly of unknown ecological significance but depend on highly specific mechanisms. This paper will give an overview on interactions between Archaea and other organisms and will point out the ecological relevance of these symbiotic processes, as long as these have been already recognized.
    Keywords: Archaea; Symbioses; ; 551
    Language: English
    Type: article , publishedVersion
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  • 5
    Publication Date: 2021-06-22
    Description: The contribution of sediments to nutrient cycling of the coastal North Sea is strongly controlled by the intensity of fluxes across the sediment water interface. Pore‐water advection is one major exchange mechanism that is well described by models, as it is determined by physical parameters. In contrast, biotransport (i.e., bioirrigation, bioturbation) as the other major transport mechanism is much more complex. Observational data reflecting biotransport, from the German Bight for example, is scarce. We sampled the major sediment provinces of the German Bight repeatedly over the years from 2013 to 2019. By employing ex situ whole core incubations, we established the seasonal and spatial variability of macrofauna‐sustained benthic fluxes of oxygen and nutrients. A multivariate, partial least squares analysis identified faunal activity, in specifically bioturbation and bioirrigation, alongside temperature, as the most important drivers of oxygen and nutrient fluxes. Their combined effect explained 63% of the observed variability in oxygen fluxes, and 36–48% of variability in nutrient fluxes. Additional 10% of the observed variability of fluxes were explained by sediment type and the availability of plankton biomass. Based on our extrapolation by sediment provinces, we conclude that pore‐water advection and macrofaunal activity contributed equally to the total benthic oxygen uptake in the German Bight.
    Description: Bundesministerium für Bildung und Forschung http://dx.doi.org/10.13039/501100002347
    Keywords: 551 ; southern North Sea ; coastal sediments ; macrofauna ; bioturbation ; bioirrigation ; organic matter turnover
    Type: article
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  • 6
    Publication Date: 2021-03-29
    Description: The syntrophic community between anaerobic methanotrophic archaea and sulfate reducing bacteria forms thick, black layers within multi-layered microbial mats in chimney-like carbonate concretions of methane seeps located in the Black Sea Crimean shelf. The microbial consortium conducts anaerobic oxidation of methane, which leads to the formation of mainly two biomineral by-products, calcium carbonates and iron sulfides, building up these chimneys. Iron sulfides are generated by the microbial reduction of oxidized sulfur compounds in the microbial mats. Here we show that sulfate reducing bacteria deposit biogenic iron sulfides extra- and intracellularly, the latter in magnetosome-like chains. These chains appear to be stable after cell lysis and tend to attach to cell debris within the microbial mat. The particles may be important nuclei for larger iron sulfide mineral aggregates.
    Keywords: Biogenic Iron Minerals; Methane; Microbial Mat ; 551 ; Anaerobiosis ; Archaea ; Bacteria ; Black Sea ; Calcium Carbonate ; Geologic Sediments ; Iron ; Methane ; Microbial Consortia ; Oxidation-Reduction ; Seawater ; Sulfates ; Sulfides ; Sulfur-Reducing Bacteria
    Language: English , English
    Type: article , publishedVersion
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  • 7
    Publication Date: 2021-03-29
    Description: Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated. Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50-70% of the enzyme was localized close to the cytoplasmic membrane, which implies "facultative" membrane association of the enzyme. This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms.
    Description: Open-Access-Publikationsfonds 2013
    Keywords: Methyl-Coenzyme M Reductase; Metabolic Marker; Methanogenic Archaea ; 551 ; Anaerobiosis ; Antibodies ; Archaea ; Cell Membrane ; Cytoplasm ; Methane ; Oxidation-Reduction ; Oxidoreductases ; Staining and Labeling
    Language: English , English
    Type: article , publishedVersion
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