ALBERT

Description: Microbial mats are modern examples of the earliest microbial communities known. Among the best studied are microbial mats growing in hypersaline ponds managed for the production of salt by Exportadora de Sal, S.A. de C.V., Guerrero Negro, Baja California Sur, Mexico. In May, 2001, we collected mats from Ponds 4 and 5 in this system and returned them to Ames Research Center, where they have been maintained for a period of over nine months. We report here on both the ecophysiological changes occurring in the mats over that period of time as well as the facility in which they were incubated. Mats (approximately 1 sq. meter total area) were incubated in a greenhouse facility modified to provide the mats with natural levels of visible and ultraviolet radiation as well as constantly flowing, temperature-controlled water. Two replicated treatments were maintained, a 'high salinity' treatment (about 120 ppt) and a 'low salinity' treatment (about 90 ppt). Rates of net biological activity (e.g., photosynthesis, respiration, trace gas production) in the mats were relatively constant over the several months, and were similar to rates of activity measured in the field. However, over the course of the incubation, mats in both treatments changed in physical appearance. The most obvious change was that mats in the higher salinity treatments developed a higher proportion of carotenoid pigments (relative to chlorophyll), resulting in a noticeably orange color in the high salinity mats. This trend is also seen in the natural salinity gradient present at the field site. Changes in the community composition of the mats, as assayed by denaturing gradient gel electrophoresis (DGGE), as well as biomarker compounds produced in the mats were also monitored. The degree to which the mats kept in the greenhouse changed from the originally collected mats, as well as differences between high and low salinity mats will be discussed. Additional information is contained in the original extended abstract.

Description: Physiological and molecular phylogenetic approaches were used to investigate variation among 12 cyanobacterial strains in their tolerance of sulfide, an inhibitor of oxygenic photosynthesis. Cyanobacteria from sulfidic habitats were found to be phylogenetically diverse and exhibited an approximately 50-fold variation in photosystem II performance in the presence of sulfide. Whereas the degree of tolerance was positively correlated with sulfide levels in the environment, a strain's phenotype could not be predicted from the tolerance of its closest relatives. These observations suggest that sulfide tolerance is a dynamic trait primarily shaped by environmental variation. Despite differences in absolute tolerance, similarities among strains in the effects of sulfide on chlorophyll fluorescence induction indicated a common mode of toxicity. Based on similarities with treatments known to disrupt the oxygen-evolving complex, it was concluded that sulfide toxicity resulted from inhibition of the donor side of photosystem II.

Description: The Upper Atmosphere Research Satellite (UARS), designed, built, integrated, tested, and operated by NASA and Martin Marietta is a low-Earth orbiting, Earth-observing spacecraft which was launched via Space Shuttle Discovery on September 12, 1991 and deployed three days later. The Modular Power Subsystem (MPS) onboard the satellite is equipped with three NASA Standard 50 Ampere-hour (Ah) nickel-cadmium (NiCd) batteries. McDonnell Douglas Electronics Systems Company fabricated the MPS, and batteries from Gates Aerospace Batteries cells. Nominal battery performance was achieved for the first four months of spacecraft operation. First evidence of anomalous battery performance was observed in January 1992, after the first maximum beta angle (low Depth of Discharge) period. Since then, the Flight Operations Team (FOT), under the direction of Goddard Space Flight Center's UARS Project and Space Power Application Branch, has monitored and managed battery performance by adjusting solar array offset angle, conducting periodic deep discharge, and controlling battery recharge ratio. This paper covers a brief overview of the UARS, the FOT's operational battery management, and the observed spacecraft battery performance.

Description: PRIME is a system to be used by an intelligent machine to allow it to operate in an abstract but uncertain (or stochastic) environment. It maintains a model of the effects of the machine's actions in the form of a rule base, which is induced from experience. This bottom-up approach to rule development allows the model to adapt to changes in the environment. Each rule consists of a condition under which the rule is active, an action, the effect of the action on the environment, and an estimate of the probability of this effect occurring. The effect probabilities are used to model the uncertainty in the environment, permitting multiple possible effects for a single action under a particular set of conditions. The objective of the intelligent machine is to satisfy user-specified goals with maximum probability of success. PRIME fulfills this requirement in two ways: it continuously updates the rule base with the most recent information, to ensure the validity of the model; and it generates plans which have the maximum probability of achieving the goals, based on the probability estimates in the rule base. PRIME is composed of three main processes: exploration, generalization, and planning. In exploration, the machine executes various randomly chosen actions, observes the effects on the environment, and updates the rule base accordingly. This process is used to develop the rule base in simulation, as well as to supplement the current knowledge during normal operation. Generalization is the procedure used to induce general rules from experience, which is encoded in the form of specific rules. These general rules extend the machine's knowledge to situations which have not been encountered yet, thereby increasing the capability of the machine to plan effectively. Planning is the process of constructing an optimal sequence of actions to satisfy a goal, using the rule base to predict the effects of these actions and to determine the probability of success of the plan. The rule representation and many other data structures were specifically chosen to maximize the efficiency of these processes. A simulated environment was designed to test the performance of PRIME. The results of experimentation were largely negative. The main problem was that the domain coverage of the rules was inadequate for the number of rules stored in the rule base, due to redundancies in general rules and numerous rules covering ineffective actions. It was determined that a more efficient generalization, and some form of goal-directed exploration, are necessary in order to solve most of the current deficiencies in PRIME.

Description: Understanding how the function of extant microorganisms has recorded both their evolutionary histories and their past interactions with the environment is a stated goal of astrobiology. We are taking a multidisciplinary approach to investigate the diversification of sulfide tolerance mechanisms in the cyanobacteria, which vary both in their degree of exposure to sulfide and in their capacity to tolerate this inhibitor of photosynthetic electron transport. Since conditions were very reducing during the first part of Earth's history and detrital sulfides have been found in Archean sediments, mechanisms conferring sulfide tolerance may have been important for the evolutionary success of the ancestors of extant cyanobacteria. Two tolerance mechanisms have been identified in this group: (1) resistance of photosystem II, the principal target of sulfide toxicity; and (2) maintenance of the ability to fix carbon despite photosystem II inhibition by utilizing sulfide as an electron donor in photosystem I - dependent, anoxygenic photosynthesis. We are presently collecting comparative data on aspects of sulfide physiology for laboratory clones isolated from a variety of habitats. These data will be analyzed within a phylogenetic framework inferred from molecular sequence data collected for these clones to test how frequently different mechanisms of tolerance have evolved and which tolerance mechanism evolved first. In addition, by analyzing these physiological data together with environmental sulfide data collected from our research sites using microelectrodes, we can also test whether the breadth of an organism's sulfide tolerance can be predicted from the magnitude of variation in environmental sulfide concentration it has experienced in its recent evolutionary past and whether greater average sulfide concentration and/or temporal variability in sulfide favors the evolution of a particular mechanism of sulfide tolerance.

Description: The NASA Engineering Safety Center (NESC) Systems Engineering (SE) Technical Discipline Team (TDT) initiated the Model Based Systems Engineering (MBSE) Pathfinder effort in FY16. The goals and objectives of the MBSE Pathfinder include developing and advancing MBSE capability across NASA, applying MBSE to real NASA issues, and capturing issues and opportunities surrounding MBSE. The Pathfinder effort consisted of four teams, with each team addressing a particular focus area. This paper focuses on Pathfinder team 1 with the focus area of architectures and mission campaigns. These efforts covered the timeframe of February 2016 through September 2016. The team was comprised of eight team members from seven NASA Centers (Glenn Research Center, Langley Research Center, Ames Research Center, Goddard Space Flight Center IV&V Facility, Johnson Space Center, Marshall Space Flight Center, and Stennis Space Center). Collectively, the team had varying levels of knowledge, skills and expertise in systems engineering and MBSE. The team applied their existing and newly acquired system modeling knowledge and expertise to develop modeling products for a campaign (Program) of crew and cargo missions (Projects) to establish a human presence on Mars utilizing In-Situ Resource Utilization (ISRU). Pathfinder team 1 developed a subset of modeling products that are required for a Program System Requirement Review (SRR)/System Design Review (SDR) and Project Mission Concept Review (MCR)/SRR as defined in NASA Procedural Requirements. Additionally, Team 1 was able to perform and demonstrate some trades and constraint analyses. At the end of these efforts, over twenty lessons learned and recommended next steps have been identified.

Description: Component performance assessment testing for a, pressure-fed earth storable bipropellant rocket engine was successfully completed at Aerojet's Redmond test facility. The primary goal of the this development project is to increase the specific impulse of an apogee class bi-propellant engine to greater than 330 seconds with nitrogen tetroxide and monomethylhydrazine propellants and greater than 335 seconds with nitrogen tetroxide and hydrazine. The secondary goal of the project is to take greater advantage of the high temperature capabilities of iridium/rhenium chambers. In order to achieve these goals, the propellant feed pressures were increased to 400 psia, nominal, which in turn increased the chamber pressure and temperature, allowing for higher c*. The tests article used a 24-on-24 unlike doublet injector design coupled with a copper heat sink chamber to simulate a flight configuration combustion chamber. The injector is designed to produce a nominal 200 lbf of thrust with a specific impulse of 335 seconds (using hydrazine fuel). Effect of Chamber length on engine C* performance was evaluated with the use of modular, bolt-together test hardware and removable chamber inserts. Multiple short duration firings were performed to characterize injector performance across a range of thrust levels, 180 to 220 lbf, and mixture ratios, from 1.1 to 1.3. During firing, ignition transient, chamber pressure, and various temperatures were measured in order to evaluate the performance of the engine and characterize the thermal conditions. The tests successfully demonstrated the stable operation and performance potential of a full scale engine with a measured c* of XXXX ft/sec (XXXX m/s) under nominal operational conditions.

Description: Photosynthetic microbial mat communities were obtained from marine hypersaline saltern ponds, maintained in a greenhouse facility, and examined for the effects of salinity variations. Because these microbial mats are considered to be useful analogs of equivalent ancient marine communities, they offer insights about evolutionary events during the greater than 3 billion year time interval wherein mats co-evolved with Earth's geosphere and atmosphere. Although photosynthetic mats can be highly dynamic and exhibit extremely high activity, the mats in the present study have been maintained for more than one year with relatively minor changes. The major groups of microorganisms, as assayed using microscopic, genetic, and biomarker methodologies, are essentially the same as those in the original field samples. Field and greenhouse mats were similar with respect to rates of exchange of oxygen and dissolved inorganic carbon across the mat-water interface, both during the day and at night. Field and greenhouse mats exhibited similar rates of efflux of methane and hydrogen. Manipulations of salinity in the water overlying the mats produced changes in the community that strongly resemble those observed in the field. A collaboratory testbed and an array of automated features are being developed to support remote scientific experimentation with the assistance of intelligent software agents. This facility will permit teams of investigators to explore ancient environmental conditions that are rare or absent today but might have influenced the early evolution of these photosynthetic ecosystems.

Description: The primary objective of this research project is to test the hypothesis that corticosteroids contribute to the adverse skeletal effects of space flight. To achieve this objective, serum corticosteroids, which are known to increase during space flight, must be maintained at normal physiologic levels in flight rats by a combination of adrenalectomy and corticosteroid supplementation via implanted hormone pellets. Bone analyses in these animals will then be compared to those of intact flight rats that, based on past experience, will undergo corticosteroid excess and bone loss during space flight. The results will reveal whether maintaining serum corticosteroids at physiologic levels in flight rats affects the skeletal abnormalities that normally develop during space flight. A positive response to this question would indicate that the bone loss and decreased bone formation associated with space flight are mediated, at least in part, by corticosteroid excess.

Description: The next phase of robotic and human deep space exploration missions requires high performance, high power solar electric propulsion systems for large-scale science missions and cargo transportation. Aerojet Rocketdyne's Advanced Electric Propulsion System (AEPS) program is completing development and qualification of a 13kW flight EP system to support NASA exploration. The first use of the AEPS is planned for the NASA Power & Propulsion Element, which is the first element of NASA's cis-lunar Gateway. The flight AEPS system includes a magnetically shielded long-life Hall thruster, power processing unit (PPU), and xenon flow controller (XFC). The Hall thruster, originally developed and demonstrated by NASA's Glenn Research Center and the Jet Propulsion Laboratory, operates at input powers up to 13.3kW while providing a specific impulse over 2600s at an input voltage of 600V. The power processor is designed to accommodate an input voltage range of 95 to 140V, consistent with operation beyond the orbit of Mars. The integrated system is continuously throttleable between 3 and 13.3kW. The program has completed testing of the Technology Development Units and is progressing into the Engineering Development Unit test phase and the final design phase to Critical Design Review (CDR). This paper will present the high power AEPS system capabilities, overall program and design status and the latest test results for the 13kW flight system development as well as the plans for the development and qualification effort of the EP string.