ALBERT

Description: An interdisciplinary team at the Jet Propulsion Laboratory Center for Life Detection has embarked on a project to provide in situ chemical and morphological characterization of Antarctic cryptoendolithic microbial communities. We present here in situ deep ultraviolet (UV) native fluorescence and environmental scanning electron microscopy images transiting 8.5 mm into a sandstone sample from the Antarctic Dry Valleys. The deep ultraviolet imaging system employs 224.3, 248.6, and 325 nm lasers to elicit differential fluorescence and resonance Raman responses from biomolecules and minerals. The 224.3 and 248.6 nm lasers elicit a fluorescence response from the aromatic amino and nucleic acids. Excitation at 325 nm may elicit activity from a variety of biomolecules, but is more likely to elicit mineral fluorescence. The resultant fluorescence images provide in situ chemical and morphological maps of microorganisms and the associated organic matrix. Visible broadband reflectance images provide orientation against the mineral background. Environmental scanning electron micrographs provided detailed morphological information. The technique has made possible the construction of detailed fluorescent maps extending from the surface of an Antarctic sandstone sample to a depth of 8.5 mm. The images detect no evidence of microbial life in the superficial 0.2 mm crustal layer. The black lichen component between 0.3 and 0.5 mm deep absorbs all wavelengths of both laser and broadband illumination. Filamentous deep ultraviolet native fluorescent activity dominates in the white layer between 0.6 mm and 5.0 mm from the surface. These filamentous forms are fungi that continue into the red (iron-rich) region of the sample extending from 5.0 to 8.5 mm. Using differential image subtraction techniques it is possible to identify fungal nuclei. The ultraviolet response is markedly attenuated in this region, apparently from the absorption of ultraviolet light by iron-rich particles coating the filaments. Below 8.5 mm the filamentous morphology of the upper layers gives way to punctate 1-2 micron particles evidencing fluorescent activity following excitation at both deep ultraviolet wavelengths.

Description: One of the most difficult problems of life detection is that of identifying biosignatures across a wide range of scales using multiple co-registered probes. The technique should be of equal utility across a wide range of search spaces from remote sensors probing volumes of space or planetary surfaces, visual eye or camera searches across the surface of a rock in Antarctica, low resolution microscopic scanning of a rock or a space craft in situ, or high resolution electron microscope and computerized tomography scanning of geobiological samples. We describe here an approach to this problem which derives in large part from past work done in the area of astrophysics - namely the analysis of complexity in galactic signals by data compression methods. This approach is a radically new one for geobiology and astrobiology, and allows us to assess the complexity (and thus potential biogenicity) of an object being examined. This is done by considering the information within pixels of an image (regardless the sensor used to gather the information) as an energetic system capable of description in terms of classical thermodynamics. The image data space is searched by an algorithm that judges complexity via data compression (e.g., the more compressible it is, the less complex, and vice versa) and maximum entropy as originally outlined by Shannon. At present we are implementing methods to utilize images from multiple sensors gathering different kinds of information (e.g., visible gray-scale data, color analyses, UV fluorescence, chemical information, etc). We present here preliminary data from deep UV fluorescence and ESEM (Environmental Scanning Electron Microscope) images from a layered cryptoendolithic community of an Antarctic rock.

Description: Redistribution and depolarization of near-resonant radiation is studied for the He 2(1P)-3(1D) line (668 nm) and some data for H-alpha (656 nm) are also presented. A Nd:YAG pumped dye laser, with a bandwidth of 0.1/cm and a power density of 100 kW/sq cm, is used in the experiment. The transitions have lower levels with natural line widths that are large compared with their upper-level natural widths, and, under experimental conditions, their collisional widths are also small. The ratio of fluorescence to Rayleigh intensity is measured and the results indicate that while the Rayleigh emission has the same temporal development as the laser pulse (5 ns FWHM), the fluorescent intensity has a decay time determined by the radiative decay lifetime and by collisional quenching. The prediction that redistribution of radiation occurs in the absence of collisions for transitions having significant lower-level radiative widths is confirmed. Depolarization rates by collisions with helium are also inferred from the measurements.

Description: In recent years, we have developed two types of high resolution, multi-channel time interval counters. In the NIST two-way time transfer MODEM application, the counter is designed for operating primarily in the interrupt-driven mode, with 3 start channels and 3 stop channels. The intended start and stop signals are 1 PPS, although other frequencies can also be applied to start and stop the count. The time interval counters used in the NIST Frequency Measurement and Analysis System are implemented with 7 start channels and 7 stop channels. Four of the 7 start channels are devoted to the frequencies of 1 MHz, 5 MHz or 10 MHz, while triggering signals to all other start and stop channels can range from 1 PPS to 100 kHz. Time interval interpolation plays a key role in achieving the high resolution time interval measurements for both counters. With a 10 MHz time base, both counters demonstrate a single-shot resolution of better than 40 ps, and a stability of better than 5 x 10(exp -12) (sigma(sub chi)(tau)) after self test of 1000 seconds). The maximum rate of time interval measurements (with no dead time) is 1.0 kHz for the counter used in the MODEM application and is 2.0 kHz for the counter used in the Frequency Measurement and Analysis System. The counters are implemented as plug-in units for an AT-compatible personal computer. This configuration provides an efficient way of using a computer not only to control and operate the counters, but also to store and process measured data.

Description: This paper covers the verification and protocol validation for distributed computer and communication systems using a computer aided testing approach. Validation and verification make up the so-called process of conformance testing. Protocol applications which pass conformance testing are then checked to see whether they can operate together. This is referred to as interoperability testing. A new comprehensive approach to protocol testing is presented which address: (1) modeling for inter-layer representation for compatibility between conformance and interoperability testing; (2) computational improvement to current testing methods by using the proposed model inclusive of formulation of new qualitative and quantitative measures and time-dependent behavior; (3) analysis and evaluation of protocol behavior for interactive testing without extensive simulation.

Description: The Lynx arc, with a redshift of 3.357, was discovered during spectroscopic follow-up of the z=0.570 cluster RX J0848+4456 from the ROSAT Deep Cluster Survey. The arc is characterized by a very red R - K color and strong, narrow emission lines.

Description: The amino acid distributions in the Murchison carbonaceous chondrite, Mars meteorite ALH84001, and ice from the Allan Hills region of Antarctica are shown, using a multivariate technique known as Principal Component Analysis (PCA), to be statistically distinct from the average amino acid compostion of 101 terrestrial protein superfamilies.

Description: We have elecited a reliable glucose signature in mammalian physiological ranges using near infrared Raman laser excitation at 785 nm and multivariate analysis. In a recent series of experiments we measured glucose levels in an artificial aqueous humor in the range from 0.5 to 13X normal values.