ALBERT

Description: Approximate ground state wave function used in variational principle for second order energy

Description: Hypervirial theorems for variational wave function in scattering theory

Description: Non-intrusive optical measurement techniques have been examined in the context of developing robust instruments which can routinely yield data of engineering utility in high speed turbomachinery test rigs. The engineering requirements of such a measurement are presented. Of particular interest were approaches that provide both velocity and state-variable information in order to be able to completely characterize transonic flowfields. Consideration of all of the requirements lead to the selection of particle image velocimetry (PIV) for the approach to velocity measurement while laser induced fluorescence of oxygen (O2 LIF) appeared to offer the most promise for gas temperature measurement. A PIV system was developed and demonstrated on a transonic turbine stage in the MIT blowdown turbine facility. A comprehensive data set has been taken at one flow condition. Extensive calibration established the absolute accuracy of the velocity measurements to be 3-5 %. The O2 LIF proved less successful. Although accurate for low speed flows, vibrational freezing of O2 prevented useful measurements in the transonic, 300-600 K operating range of interest here.

Description: Extended spaceflight under microgravity conditions leads to significant atrophy of weight-bearing muscles. Atrophy and hypertrophy are the extreme outcomes of the high degree of plasticity exhibited by skeletal muscle. Stimuli which control muscle plasticity include neuronal, hormonal, nutritional, and mechanical inputs. The mechanical stimulus for muscle is directly related to the work or exercise against a load performed. Little or no work is performed by weight-bearing muscles under microgravity conditions. A major hypothesis is that focal adhesion kinase (FAK) which is associated with integrin at the adherens junctions and costa meres of all skeletal muscles is an integral part of the major mechanism for molecular signaling upon mechanical stimulation in all muscle fibers. Additionally, we propose that myotonic protein kinase (DMPK) and dystrophin (DYSTR) also participate in distinct mechanically stimulated molecular signaling pathways that are most critical in type I and type II muscle fibers, respectively. To test these hypotheses, we will use the paradigms of hindlimb unloading and overloading in mice as models for microgravity conditions and a potential exercise countermeasure, respectively, in mice. We expect that FAK loss-of-function will impair hypertrophy and enhance atrophy in all skeletal muscle fibers whereas DYSTR and DMPK loss-of-function will have similar but more selective effects on Type IT and Type I fibers, respectively. Gene expression will be monitored by muscle-specific creatine kinase M promoter-reporter construct activity and specific MRNA and protein accumulation in the soleus (type I primarily) and plantaris (type 11 primarily) muscles. With these paradigms and assays, the following Specific Project Aims will be tested in genetically altered mice: 1) identify the roles of DYSTR and its pathway; 2) evaluate the roles of the DMPK and its pathway; 3) characterize the roles of FAK and its pathway and 4) genetically analyze the mechanisms and interactions between the FAK, DYSTR, and DMPK-associated pathways in single and specific combinations of mutants. The identification of potential signaling mechanisms may permit future development of pharmacological countermeasures for amelioration and prevention of the microgravity-induced atrophy in extended spaceflight, and the analysis of both overloading and unloading paradigms may provide further support for development of exercise-based countermeasures. Understanding the basic mechanisms of molecular signaling in muscle plasticity may aid our understanding and treatment of skeletal muscle atrophy not only in spaceflight but in similar problems of the aging population, in prolonged bed rest, and in cachexia associated with chronic disease.

Description: Computer simulation of atmospheric drag for testing trajectory of artificial satellite or space probe

Description: Accuracy of ibm 7094 fortran iv subroutines

Description: The remarkable order of striated muscle is the result of a complex series of protein interactions at different levels of organization. Within muscle, the thick filament and its major protein myosin are classical examples of functioning protein machines. Our understanding of the structure and assembly of thick filaments and their organization into the regular arrays of the A-band has recently been enhanced by the application of biochemical, genetic, and structural approaches. Detailed studies of the thick filament backbone have shown that the myosins are organized into a tubular structure. Additional protein machines and specific myosin rod sequences have been identified that play significant roles in thick filament structure, assembly, and organization. These include intrinsic filament components, cross-linking molecules of the M-band and constituents of the membrane-cytoskeleton system. Muscle organization is directed by the multistep actions of protein machines that take advantage of well-established self-assembly relationships. Copyright 1999 John Wiley & Sons, Inc.

Description: Polar mount and azimuth-elevation mount antennas evaluated for spacecraft tracking in terms of relative static and dynamic coverage

Description: Radiation effect on liquid metal corrosion

Description: Chemical corrosion by liquid metals