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  • Data  (6)
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  • 1
    Publication Date: 2024-01-26
    Description: We assessed the responses of solitary cells of Arctic Phaeocystis pouchetii (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry.
    Keywords: Alkalinity, total; Bottle incubation; calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006); calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic nitrogen (PON) quota; calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota; Carbon, inorganic, dissolved; Carbon dioxide, partial pressure; Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001); Coulter counter, Beckman Coulter, Multisizer 3; DATE/TIME; Electron transport rate, relative; Elemental analyzer, EuroVector, EuroEA; EXP; Experiment; Experimental treatment; Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016); Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201; Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996); Fram Strait; Identification; Initial slope of the photosynthesis-irradiance curve; Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light; Light; Light acclimation index; Maximum photosynthesis rate, oxygen, per chlorophyll a; model simulation; pCO2; pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system; pH; pH 826 mobile handheld device, with Aquatrode Plus, Metrohm; Phaeocystis_pouchetii_PS78; Phaeocystis pouchetii; Phaeocystis pouchetii, carbon, organic, particulate/nitrogen, organic, particulate ratio; Phaeocystis pouchetii, chlorophyll a/carbon, organic, particulate ratio; Phaeocystis pouchetii, chlorophyll a quota per cell; Phaeocystis pouchetii, growth rate; Phaeocystis pouchetii, particulate organic carbon production per cell; Phaeocystis pouchetii, particulate organic carbon quota per cell; Phaeocystis pouchetii, particulate organic nitrogen production per cell; Phaeocystis pouchetii, particulate organic nitrogen quota per cell; Phytoplankton; RCP8.5; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Temperature; Temperature, water; Thermometer, internal, Aquatrode Plus, Metrohm; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: temperature; Type of study; Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR
    Type: Dataset
    Format: text/tab-separated-values, 908 data points
    Location Call Number Expected Availability
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  • 2
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    PANGAEA
    In:  Institut für Umweltphysik, Universität Heidelberg
    Publication Date: 2024-02-01
    Keywords: 23-10; ALTITUDE; Bromine monoxide, vertical column density; Bromine monoxide, vertical column density, error; Bromine monoxide mixing ratio; Bromine monoxide mixing ratio, error; CT; DATE/TIME; Eastern Tropical North Atlantic; Iodine monoxide, vertical column density; Iodine monoxide, vertical column density, error; Iodine monoxide volume mixing ratios; Iodine monoxide volume mixing ratios, error; LATITUDE; LONGITUDE; Multi-AXis Differential Optical Absorption Spectrometer (MAX-DOAS); POS399/3; POS399/3-track; Poseidon; SOPRAN; Surface Ocean Processes in the Anthropocene; Underway cruise track measurements
    Type: Dataset
    Format: text/tab-separated-values, 984 data points
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  • 3
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    PANGAEA
    In:  Institut für Umweltphysik, Universität Heidelberg
    Publication Date: 2024-02-01
    Keywords: 23-10; ALTITUDE; Bromine monoxide, vertical column density; Bromine monoxide, vertical column density, error; Bromine monoxide mixing ratio; Bromine monoxide mixing ratio, error; CT; DATE/TIME; Eastern Tropical North Atlantic; Iodine monoxide, vertical column density; Iodine monoxide, vertical column density, error; Iodine monoxide volume mixing ratios; Iodine monoxide volume mixing ratios, error; LATITUDE; LONGITUDE; Multi-AXis Differential Optical Absorption Spectrometer (MAX-DOAS); POS399/2; POS399/2-track; Poseidon; SOPRAN; Surface Ocean Processes in the Anthropocene; Underway cruise track measurements
    Type: Dataset
    Format: text/tab-separated-values, 4448 data points
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  • 4
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    PANGAEA
    In:  Supplement to: Hoins, Mirja; Eberlein, Tim; Großmann, Christian H; Brandenburg, Karen; Reichart, Gert-Jan; Rost, Björn; Sluijs, Appy; Van de Waal, Dedmer B (2016): Combined effects of ocean acidification and light or nitrogen availabilities on 13C fractionation in marine dinoflagellates. PLoS ONE, 11(5), e0154370, https://doi.org/10.1371/journal.pone.0154370
    Publication Date: 2024-03-15
    Description: Along with increasing oceanic CO2 concentrations, enhanced stratification constrains phytoplankton to shallower upper mixed layers with altered light regimes and nutrient concentrations. Here, we investigate the effects of elevated pCO2 in combination with light or nitrogen-limitation on 13C fractionation (epsilon p) in four dinoflagellate species. We cultured Gonyaulax spinifera and Protoceratium reticulatum in dilute batches under low-light (LL) and high-light (HL) conditions, and grew Alexandrium fundyense and Scrippsiella trochoidea in nitrogen-limited continuous cultures (LN) and nitrogen-replete batches (HN). The observed CO2-dependency of epsilon p remained unaffected by the availability of light for both G. spinifera and P. reticulatum, though at HL epsilon p was consistently lower by about 2.7 per mil over the tested CO2 range for P. reticulatum. This may reflect increased uptake of (13C-enriched) bicarbonate fueled by increased ATP production under HL conditions. The observed CO2-dependency of epsilon p disappeared under LN conditions in both A. fundyense and S. trochoidea. The generally higher epsilon p under LN may be associated with lower organic carbon production rates and/or higher ATP:NADPH ratios. CO2-dependent epsilon p under non-limiting conditions has been observed in several dinoflagellate species, showing potential for a new CO2-proxy. Our results however demonstrate that light- and nitrogen-limitation also affect epsilon p, thereby illustrating the need to carefully consider prevailing environmental conditions.
    Keywords: Alexandrium fundyense; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate, per cell; Carbon, organic, particulate, standard deviation; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chromista; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gonyaulax spinifera; Growth/Morphology; Growth rate; Growth rate, standard deviation; Isotopic fractionation, during photosynthis; Isotopic fractionation, during photosynthis, standard deviation; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; Myzozoa; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phytoplankton; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Protoceratium reticulatum; Registration number of species; Salinity; Scrippsiella trochoidea; Single species; Species; Temperature, water; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 1008 data points
    Location Call Number Expected Availability
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  • 5
    Publication Date: 2024-03-15
    Description: We assessed the responses of solitary cells of Arctic Phaeocystis pouchetii (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-01-20.
    Keywords: Alkalinity, total; Aragonite saturation state; Arctic; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottle incubation; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006); calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic nitrogen (PON) quota; calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, particulate, per cell; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Chromista; Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001); Coulter counter, Beckman Coulter, Multisizer 3; Date/time end; Date/time start; Electron transport rate, relative; Elemental analyzer, EuroVector, EuroEA; EXP; Experiment; Experimental treatment; Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016); Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201; Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996); Fram Strait; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth rate; Haptophyta; Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light; Laboratory experiment; Light; Light acclimation index; Maximum photosynthesis rate, oxygen, per chlorophyll a; Nitrogen, organic, particulate, per cell; OA-ICC; Ocean Acidification International Coordination Centre; Open ocean; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon production per cell; pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system; Pelagos; pH; pH 826 mobile handheld device, with Aquatrode Plus, Metrohm; Phaeocystis_pouchetii_PS78; Phaeocystis pouchetii; Phytoplankton; Polar; Primary production/Photosynthesis; Production of particulate organic nitrogen; Replicate; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Temperature; Temperature, water; Thermometer, internal, Aquatrode Plus, Metrohm; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: temperature; Type of study; Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR
    Type: Dataset
    Format: text/tab-separated-values, 1580 data points
    Location Call Number Expected Availability
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  • 6
    Publication Date: 2024-04-13
    Keywords: CT; DATE/TIME; Height above sea level; Iodine monoxide, vertical column density; Iodine monoxide, vertical column density, error; Iodine monoxide volume mixing ratios; Iodine monoxide volume mixing ratios, error; LATITUDE; LONGITUDE; SO202/2; SO202/2-track; Sonne; TransBrom; Underway cruise track measurements; West Pacific
    Type: Dataset
    Format: text/tab-separated-values, 2780 data points
    Location Call Number Expected Availability
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