Publication Date:
2024-03-15
Description:
Increasing use of fish feed containing the chitin synthesis inhibiting anti-parasitic drug diflubenzuron (DFB) in salmon aquaculture has raised concerns over its impact on coastal ecosystems. Larvae of Northern shrimp (Pandalus borealis) were exposed to DFB medicated feed under Control conditions (7.0 °C, pH 8.0) and under Ocean Acidification and Warming conditions (OAW, 9.5 °C and pH 7.6). Two weeks' exposure to DFB medicated feed caused significantly increased mortality. The effect of OAW and DFB on mortality of shrimp larvae was additive; 10% mortality in Control, 35% in OAW, 66% in DFB and 92% in OAW + DFB. In OAW + DFB feeding and swimming activity were reduced for stage II larvae and none of the surviving larvae developed to stage IV. Two genes involved in feeding (GAPDH and PRLP) and one gene involved in moulting (DD9B) were significantly downregulated in larvae exposed to OAW + DFB relative to the Control. Due to a shorter intermoult period under OAW conditions, the OAW + DFB larvae were exposed throughout two instead of one critical pre-moult period. This may explain the more serious sub-lethal effects for OAW + DFB than DFB larvae. A single day exposure at 4 days after hatching did not affect DFB larvae, but high mortality was observed for OAW + DFB larvae, possibly because they were exposed closer to moulting. High mortality of shrimp larvae exposed to DFB medicated feed, indicates that the use of DFB in salmon aquaculture is a threat to crustacean zooplankton.
Keywords:
Alkalinity, total; Animalia; Aragonite saturation state; Arthropoda; Behaviour; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; EXP; Experiment; Feeding rate per individual; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression, fold change, relative; Gene expression, fold change, relative, standard error; Gene expression (incl. proteomics); Gene name; Hillefjord; Individuals; Laboratory experiment; Mortality/Survival; North Atlantic; Number; OA-ICC; Ocean Acidification International Coordination Centre; Other; Pandalus borealis; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; Percentage; Percentage, standard deviation; pH; Registration number of species; Salinity; Single species; Species; Stage; Swimming activity, beam breaks per hour; Swimming activity, standard deviation; Temperate; Temperature; Temperature, water; Time in days; Treatment; Type; Uniform resource locator/link to reference; Zooplankton
Type:
Dataset
Format:
text/tab-separated-values, 3888 data points
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