ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (55)
  • 1
    facet.materialart.
    Unknown
    Identification and extraction of streptococcusis gene from streptococcus iniae and recording in Gene Bank (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: S. iniae is an important pathogen in both marin animals and humans, causing systemic infections in these hosts. The symptoms of infections are very similar to symptoms of infections caused by human¬specific streptococcal pathogens. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in s. iniae. We aim to isolate S. iniae and cloning phosphoglucomutase gene. S. iniae grew on sheep blood agar as mucoid and beta- hemolytic colonies after 24 h of incubation at 37C. Then confirmed it by sensitive and rapid method of PCR. The pgm gene was amplified successfully and cloned in pTZ57R cloning vector.The recombinant plasmid was sub cloned into pETDuet-l expression vector by rectriction enzymes and confirmed by PCR . Although S. iniae is a pathogen of economic importance, relatively little is known regarding mechanisms of its pathogenesis and few specific virulence determinants have been established. These include the enzyme phosphoglucomutase which contributes to cell wall integrity and resistance to antimicrobial peptides. Thus, this enzyme is the best choice to product the vaccine and more investigations.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Streptococcusis ; Gene ; Streptococcus iniae ; Gene Bank ; S. iniae ; Pathogen ; Enzyme ; Phosphoglucomutase ; PCR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 33pp.
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 2
    facet.materialart.
    Unknown
    Comprehensive study on assessment of Sturgeons population genetic structure in the Caspian Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The population genetic structure of five Caspian Sea sturgeon species was investigated. Totally 1121 samples of caudal and unault's fin tissue of the sturgeons (Acipenser persicus, A. gueldenstaedtii, A. stellatus, A. nudiventris and Huso huso) were collected from the Volga River (Russia), Ural River (Kazakhstan), Kura River (Azerbaijan), Sepidrud River and the coastline of the south Caspian in the Iranian waters as well as from the sampling stations selected for the marine survey for sturgeon stock assessment in the Caspian Sea. All samples were stored in 96% ethyl alcohol and transferred to the genetic laboratory of the International Sturgeon Research Institute. Genomic DNA was extracted using phenol-chloroform method. The quality and quantity of DNA was assessed by Agarose gel (1%) electrophoresis and spectrophotometry. The population genetic structure of Ship and Persian sturgeon was studied using both PCR-RFLP (D-loop and ND5/6 gene) and microsatellite technique and that of H. huso, A. stellatus and A. persicus were studied using microsatellite technique. After amplification of genes using PCR, the RFLP technique was used to digest mtDNA using restriction enzyme. The PCR products were electrophoresed on 6% sequencing polyacrylamide gels followed by silver nitrate staining. Data for PCR-RFLP were analyzed using REAP program and those from microsatellite technique were analyzed using Gene Alex. Population genetic parameters including allele frequency, expected and observed heterozygosity, effective allele, Shannon's index were determined. Genetic identity and distance were calculated following Nei criteria and Hardy Weinberg equilibrium was tested based on X2 and analysis of molecular variance (AMOVA) using Reap and Gen Alex at 99% confidence limit. Phylogenetic relationship was determined and drawn using TFPGA program. The population genetic structure and genetic diversity of the 1121 sturgeon specimens were determined. Three independent populations were identified for Acipenser persicus (two populations in the south Caspian in the Iranian waters and one in the north Caspian). Three independent populations were identified for A. gueldenstaedtii (Volga, Ural and South Caspian populations) using the microsatellite technique. Population genetic structure using PCR-RFLP revealed no genetic differentiation among the A. gueldenstaedtii specimens studied from the different regions using ND5/6 gene, while two populations (Ural and south Caspian populations) were detected for this species with the same technique using D-loop genes. Four independent populations (Volga, Ural, Kura and Sepidrud populations) were reported for A. stellatus using the microsatellite technique and four more populations which most probably belong to the autumn and spring races of the above mentioned independent populations were identified for this species. The present study also identified two populations for H. huso; The North Caspian population (in Volga and Ural Rivers) and The South Caspian population (in Golestan and Guilan regions) which were significantly different from each other (P〈0.01). The genetic population structure of A. nudiventris was studied using the microsatellite and PCR-RFLP techniques which revealed two populations for this species one in the Ural River and the other in the Sepidrud River (South Caspian). Comparison of the ND5/6 and D-loop genes studies in Russian sturgeon revealed that the D-loop gene is better than the ND5/6 genes in population's differentiation and is therefore strongly recommended for population genetic studies on sturgeons in the Caspian Sea. Genetic diversity studied using microsatellite technique was higher and more accurate as compared to that using RFLP. Nevertheless the RFLP technique was able to introduce molecular markers for the population’s species pacific identification. On developing suitable primers these studies can be speeded up and the cost of such studies can be cut down. However the drawback in using microsatellite technique for population genetic studies is that it cannot introduce a molecular marker for the identification of populations. The present study was able to introduce molecular markers to differentiate the ship sturgeon population in the south Caspian from that in the Ural River using the PCR-RFLP technique Based on the results obtained it is strongly recommended that all activities related to restocking and rehabilitation of sturgeon stocks in Iran be conducted on the basis of genetic principles. Also serious and immediate measures should be taken for the restoration and conservation of rare population of native species of Iran particularly in the Sepidrud region using genetic markers before they are become extinct.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Assessment ; Sturgeons ; Population ; Genetic ; PCR-RFLP ; Microsatellite ; Species ; Samples ; Tissue ; Acipenser persicus ; A. gueldenstaedtii ; A. stellatus ; A. nudiventris ; Huso huso ; Survey
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 329pp.
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 3
    facet.materialart.
    Unknown
    Genetic relationships of Iranian coastline ship sturgeon (Acipenser nudiventris) samples and Ural population based on microsatellite DNA (2018)
    Details
    Publication Date: 2021-05-19
    Description: This article has no abstract.
    Description: Published
    Keywords: Acipenser nudiventris ; DNA ; Genetic ; Populations ; Microsatellite
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.229-240
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 4
    facet.materialart.
    Unknown
    Population genetic studies of Liza aurata using D-Loop sequencing in the southeast and southwest coasts of the Caspian Sea (2018)
    Details
    Publication Date: 2021-05-19
    Description: Genetic diversity as an important marker of the ecological status of aquatic ecosystems is considered a unique and powerful tool to evaluate biological communities. In order to evaluate the genetic diversity among golden mullet species (Liza aurata) in the southeast and southwest coasts of the Caspian Sea by D-Loop gene sequencing, a total of 23 fin specimens of golden mullet were collected from the Gilan (Anzali area) and Golestan (Gomishan area) provinces. Total DNA from the samples was extracted by ammonium acetate method and the quality and quantity of the extracted DNA were assessed by spectrophotometery and electrophoresis. Polymerase Chain Reaction (PCR) was conducted on the target DNA and then DNA sequencing was carried out. D- loop region in mitochondrial DNA (mtDNA) of golden mullet contained 900 base pairs (bp). Phylogenetic relationships among golden mullet were calculated by MEGA software version 5.05 and divergence time was estimated using Tahjima's test. The results obtained from this study revealed that there were high genetic differences among two regions in the Gilan and Golestan provinces. Kimura 2-parameter was used for genetic distance analysis and the genetic distance recorded between Gilan and Golestan Provinces was calculated at 0.259. The high levels of FsT were observed between Gilan and Golestan Provinces which indicates that genetic differences exist among present populations (p≤.05). Based on the results obtained from the south Caspian Sea, probably two different populations of Liza aurata are living in the Gilan and Golestan Provinces.
    Description: Published
    Keywords: Genetics ; Genetic diversity ; Liza aurata ; mtDNA ; Genetic distance ; Population
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.216-227
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 5
    facet.materialart.
    Unknown
    Using RAPD markers potential to identify heritability for growth in Fenneropenaeus indicus (2018)
    Details
    Publication Date: 2021-05-19
    Description: Sampling was done using 90 post larvae which were produced by reproduction of some broodstocks of Fenneropenaeus indicus in one day and reared in the same situation for 4 months. Samples were divided into 3 groups: high, medium and low growth (based on weight and length). Genomic DNA was extracted from muscle tissue using the phenol-chloroform method. The polymerase chain reaction (PCR) was carried out using 21 RAPD loci and PCR products were separated on 3% Agarose gel. From 21 loci studied, 12 produced polymorphic bands. The most polymorphic produced bands using OPAQ 9 and the least by OPAQ 7. Search for specific markers in F. indicus one specific band was observed in the low growth group using OPAQ4. The highest genetic distance (0.457) was between the low growth group and the medium and the lowest (0.091) between high growth and medium groups, therefore the highest genetic identity (0.912) was between high growth and medium groups and the lowest (0.633) between low growth group and the medium. Neighbor-joining resulted in two groups, the first including high and medium growth groups and the second low growth group. It appears that low growth group depended on separated population. Considering the mean weight of F1 (mean weight of 90 specimens) (16.25±1.5 g), parental generation mean weight of 15 ±1.2 and mean weight of parent 31.6 g, response to selection (R) and heritability for growth in this species were estimated to be 1.2±0.2 and 0.07±0.01 respectively.
    Description: Published
    Keywords: Feneropenaeus indicus ; RAPD marker ; Heritability ; Growth ; Iranian Fisheries
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.123-134
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 6
    facet.materialart.
    Unknown
    Short communication: Population genetic structure studies of Liza aurata based on mtDNA control region sequences analyses in the southern coasts of the Caspian Sea (2018)
    Details
    Publication Date: 2021-05-19
    Description: Nowadays many species are endangered as a result of habitat loss. Decreases in population lead to reduced genetic diversity, which can cause survival crisis in a population (Cecconi et al., 1995). Nowadays optimal management of fish stocks needs information on population structure of species that is provided to researchers through genetic science. Bereavement of science about stock composition will lead to the fracture of fisheries management and unsuitable harvest of stocks (Papasotiropoulos et al., 2007). One of the beneficial methods to demonstrate genetic diversity is haplotype analysis of the D-loop region, an index which is very important and determinant for the preservation of species. Significant genetic variation is found in the D-loop region, even among individuals within a given species. Grey mullets are not endemic species of the Caspian Sea. Juveniles of L. aurata, L. saliens and Mugil cephallus were introduced from the Black Sea into the Caspian Sea during the years 19301934. But only the introduction of L. aurata and L. saliens was successful and they adapted well to the ecological conditions of the Caspian Sea (Fazli et al., 2008).
    Description: Published
    Keywords: L. saliens ; Mugil cephallus ; Liza aurata ; Control region ; Polymorphic site ; Haplotype diversity ; Population ; Genetic ; Structure ; mtDNA
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.1341-1348
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 7
    facet.materialart.
    Unknown
    Indian White shrimp (Fenerropenaeus indicus) culture two times a year in Gwatar shrimp farm, Iran (2018)
    Details
    Publication Date: 2021-05-19
    Description: During the decade 1991-2001, culture of Indian White Shrimp grew rapidly in Iran due to high profitability, but afterwards suffered a decreasing trend with many farms still being idle after Construction. The trend occurred mainly due to (1) increase in production costs with simultaneous decrease in international shrimp market price, (2) Agro-climatic conditions that favored only one crop a year, which is not profitable, and (3) fear about spread of disease as already experienced in the case of white spot disease in Khouzestan and Bushehr provinces. Based on these facts, we aimed in our study to increase production of the shrimp per year, to reduce days of culture (DOC) in second crop through nursery system, to control food conversion ratio (FCR), and to manage shrimp production in Gwatar shrimp farming complex. Six farms were selected, and in three we applied two crops a year production system using nursery for the second crop. In other three farms one crop was harvested. Shrimps in two-crop farms were kept 52 days of the second crop in nursery and then transferred to grow-out ponds. All farms harvested before DOC 128. Mean productions per hectare in the first and second crop were 1794 and 1691kg, respectively. The FCR dropped from 1.6 in the first to 1.27 in the second crop. Total production per hectare per year reached 3485kg in two-crop farms. Shrimps in one-crop farms were harvested mainly at DOC 145. The mean production per ha/year and FCR of one-crop farms reached 2089kg and 1.65 respectively. We harvested around 47114kg of shrimps in each two-crop farm which was 17 tons more than one-crop farms. The results of this study showed that production of shrimps in two crops a year system could be continued with pre-designed schedules. We presented a time table for two crops a year culture system.
    Description: Published
    Keywords: Shrimp Farming ; Indian White Shrimp ; Fenerropenaeus indicus
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.139-148
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 8
    facet.materialart.
    Unknown
    Molecular study of the populations of Artemia partenogenetica in Iran using PCR-RFLP Method (2018)
    Details
    Publication Date: 2021-05-19
    Description: Considering the importance of genetic studies to manifest inter population differences in species, samples of Artemia partenogenetica were collected from seven inland lakes including Shoor and Inche-Borun lakes in Golestan Province, Hoze-Soltan and Namak lakes in Qom Province, Maharloo and Bakhteghan lakes in Fars Province and Mighan pool in Markazi Province. A total of 210 samples were subjected to DNA extraction by phenol-chloroform method. Primers were designed on a ribosomal fragment (16SrRNA) of the species' mtDNA sequence and the PCR was conducted on the samples. Digestion of the PCR product with approximately 1584bp lengths by 10 restriction endonuclease (AluI, EcoRI, Eco47I, HaeIII, HindIII, HinfI, MboI, MspI, RsaI, TaqI) showed 12 different haplotypes: 4 haplotypes in Shoor and Inche-Borun, 1 in Namak and Hoze-Soltan, 3 in Mighan pool, 1 in Bakhtegan and Maharloo and 3 in Maharloo. Haplotype diversity values within collected samples varied from zero in Hoze-Soltan, Namak and Bakhteghan samples to 0.7425 in Inche-Borun and Shoor while nucleotide diversity varied from zero in Hoze-Soltan, Namak and Bakhteghan, to 0.0077 in Mighan. The minimum nucleotide diversity among samples was zero between Hoze-Soltan vs. Namak and the maximum was 0.1700 between Inche-Borun and Shoor vs. Mighan. Nucleotide divergences among samples were least in Inche-Borun vs. Shoor (%-0.02) and most in Inche-Borun and Shoor vs. Mighan (%16.18), averaging to %3.40. The evolutionary distances between 12 haplotype showed that the maximum value belonged to Mighan haplotypes vs. Inche-Borun and Shoor haplotypes. Regarding the digestive patterns produced by each enzyme in the studied region, Eco47I is introduced as the population-specific marker of A. partenogenetica in Iran. Test of population differentiation based on haplotype frequencies were statistically significant (P~,0.001) with the exception of Hoze-Soltan vs. Namak and Inche-Borun vs. Shoor. We conclude that there are enough evidences in haplotypic level for dividing A. partenogenetica in Iran into five populations: Hoze-Soltan and Namak, Mighan, Maharloo, Bakhtegan, Incheh-Borun and Shoor.
    Description: Published
    Keywords: RNA ; Nucleotide sequence ; Primers ; Nucleotides ; Artemia partenogenetica ; Population Genetics ; Genetics ; DNA ; Enzymes ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.53-68
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 9
    facet.materialart.
    Unknown
    Identification of different species of squids in Oman Sea (Iranian waters) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Identification of different species of oceanic and neritic squids in Iranian waters of Oman Sea was carried out from December 1996 to February 1997. The trawl surveys were conducted during a 12-months period. Fishing was also undertaken by Mid-water and bottom trawl for species confirmation purposes in deep (200-350m) and shallow (0-100m) waters to collect enough specimens that could be used for later species identification. The RN Ferdows-I was used for sampling with an approximate hauling speed of 3.0 knots. Three oegopsid species including Ancistrocheirus lesueuri, Liocranchia reinhardti, Sthenoteuthis oualaniensis and neritic squid, Loligo duvauceli were identified. Another loliginid squid different from Loligo duvauceli was also observed. A. lesueuri (Enoploteuthidae Family) and Liocranchia reinhardti (Cranchiidae Family) are here reported from this area for the first time. Neither was any report about these two families of oegopsid squids in Oman Sea nor Persian Gulf.
    Description: Published
    Keywords: Squid ; Oceanic ; Neritic
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.63-72
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 10
    facet.materialart.
    Unknown
    Investigation of genetic structure in populations of Mnemiopsis leidyi from north and south of Caspian Sea using RAPE Method (2018)
    Details
    Publication Date: 2021-05-19
    Description: The molecular structure in populations of Mnemiopsis leidyi were examined for 200 samples from the south (Guilan, Mazandaran and Golestan provinces) and north of the Caspian Sea. DNA was extracted from M leiydi tissue by phenol-chiorophorm method and its concentration was found at 50 to 10Ong. We used the PCR method using 19 random primers (10bp). The PCR products of samples were accompanied with standard marker (50bp DNA ). To measure fragment size, samples were run on a 1% agarose gel. Ten of the ninteen primers showed polymorphism. Statistical analysis of data was performed using POPGENE software. The avarage genetic variation was 0.189 in total samples and the maximum variation was found in samples from north of the Caspian Sea. Also, The maximume genetic distance was between north of the Sea and Golestan coasts in the south (0.089). The minimum genetic distance was between Mazandaran and Guilan coasts (0.001). The UOGMA dendogram showed two clusters. The samples of Mazandaran, Guilan and Golestan coasts were placed in one cluster and samples of the north area in another. The genetic diversity was significantly different between samples of the north area and Golestan coasts (P〈0.05). We found a significant genetic divergence between some samples and therefore suggested at least two genetic groups of Mnemiopsis leidyi in the Caspian Sea.
    Description: Published
    Keywords: Mnemiopsis leidyi ; RAPD ; Diversity ; Genetic ; Populations ; DNA
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.119-126
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER (OA)
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...