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  • Articles  (103)
  • Springer  (68)
  • National Academy of Sciences  (35)
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  • Articles  (103)
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  • 1
    Electronic Resource
    Electronic Resource
    Einfluß von kationischen Polyelektrolyten auf die Reaktion von Chloracetat mit Thiosulfat (1977)
    Springer
    Colloid & polymer science 255 (1977), S. 99-99 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01449719
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  • 2
    Electronic Resource
    Electronic Resource
    Einfluß von kationischen Polyelektrolyten auf Reaktionen zwischen 2,4-Dinitrofluorbenzol und Aminosäuren bzw. Peptiden (1977)
    Springer
    Colloid & polymer science 255 (1977), S. 100-100 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01449721
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  • 3
    Electronic Resource
    Electronic Resource
    Einfluß kationischer Polyelektrolyte auf die Reaktion von 5,5′-Dithiobis(2-nitrobenzoat) mit dem CN-Ion (1977)
    Springer
    Colloid & polymer science 255 (1977), S. 100-100 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01449723
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  • 4
    Electronic Resource
    Electronic Resource
    Favourable interaction between heavy and light chains arrests the undesirable oligomerization of heavy chains in the refolding of denatured and reduced immunoglobulin G (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 929-934 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Aggregation; H chain; immunoglobulin G; L chain; protein folding; slow dialysis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Recently we developed a slow dialysis method that effectively refolds denatured and reduced immunoglobulin G (IgG) [Maeda, Ueda and Imoto (1996) Prot. Engng 9: 95-100]. This method allows both individual and simultaneous refolding of denatured and reduced H and L chains. Analysis by SDS-polyacrylamide gel electrophoresis revealed that some oligomers were formed through disulfide bonds when H chains were refolded individually. It was also shown that the extent of IgG obtained by rejoining the mixture of refolded H and L chains which had been refolded individually was similar to that obtained by refolding denatured and reduced whole IgG. The results indicated that a favourable interaction between H and L chains prevented formation of H-chain oligomers to yield intact IgG. The present results suggest a mechanism whereby individually folded chains might associate to form IgG molecules in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050113
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  • 5
    Electronic Resource
    Electronic Resource
    Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity (1999)
    Springer
    Cellular and molecular life sciences 55 (1999), S. 1187-1194 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Immunological tolerance; monomethoxypolyethylene glycol; extended blood half-life; hen egg lysozyme; immunochemistry.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The blood half-life of a protein is prolonged by conjugating a protein with a linear amphiphilic polymer, monomethoxypolyethylene glycol (mPEG). The conjugation gives a protein immunotolerogenicity; hence, it is likely that the long half-life is crucial for the tolerogenicity. We prepared a tolerogenic mPEG conjugate of hen egg lysozyme (mPEG1.5-HEL), which is conjugated 1.5-fold the molecular weight of mPEG against that of HEL, and evaluated the relationship between in vivo stability and the tolerogenicity. mPEG1.5-HEL retained immunogenicity to prime HEL-specific T cell and antibody responses and had a long blood half-life, more than 27 times that of native HEL. The tolerant state was maintained as long as mPEG1.5-HEL was detected in sera. With a decrease in the blood mPEG1.5-HEL level, the tolerant state returned gradually to the responsive state; however, reinjection of mPEG1.5-HEL again restored the tolerance. Thus, the extended blood half-life of HEL by mPEG conjugation is probably vital for establishing and maintaining the tolerant states.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050365
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  • 6
    Electronic Resource
    Electronic Resource
    πNN Dynamics in pp → pp, pp →πd and πd →πd Processes Below 1 GeV (1995)
    Springer
    Few body systems 18 (1995), S. 133-146 
    Details
    ISSN: 1432-5411
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. pp and πd scattering and pp →πd reaction processes at incident proton laboratory energies T L ≤ 1 GeV are studied in the framework of πNN dynamics. For this purpose three-body calculations are performed with the πN interaction in the P 11, P 33, S 11 and S 31 states and NN forces in the 3 S 1 -3 D 1, 1 S 0, and 3 P 2 states. In addition, the backward-going pion contribution at the πNΔ vertex, the πN-ρN coupling, the heavy-meson exchanges in the NN → NN driving term, and the effect of the off-shell structure in the πN-ρN P 33 interaction are taken into account.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s006010050007
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  • 7
    Electronic Resource
    Electronic Resource
    Quaternary structure-dependent idiotope and antigen binding of a monoclonal antibody specific for conformational epitope on type II collagen (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 51-60 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Protein-protein interaction; higher-order structure; anti-idiotypic antibody; type II collagen; autoimmune arthritis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. We previously generated a monoclonal antibody (mAb) against a putative pathogenic epitope on native type II collagen (CII) for the induction of collagen-induced arthritis in mice (mAb1), and an anti-idiotypic mAb which appears to possess the internal image of the CII epitope (mAb2). In the present study, the structural basis of the antigen/mAb1 and mAb1/mAb2 interactions was examined. When partially SH-reduced mAb1 was analysed on Western blots, only fragments containing both heavy (H) and light (L) chains were recognized by mAb2. When mAb2 was partially SH-reduced, only fragments containing both H and L chains were recognized by mAb1. H and L chains were separated from mAb1 in a reduced, denatured condition, and each chain and a mixture of the two were refolded. mAb2 reacted specifically to the renatured whole IgG molecule of mAb1, but not to the refolded L or to H chains. Recombinant single chain Fv (scFv) generated from mAb1 and mAb2 had properties of the original mAbs, whereas genetical ly constructed chimeric scFvs, consisting of VH from mAb1 and an irrelevant VL , or VL of mAb1 and an irrelevant VH , did not react either to CII or to mAb2. Thus, interactions among CII, mAb1 and mAb2 appear to depend on quaternary structures containing different protein subunits. These observations support the internal image property of the mAb2. In addition, this dependency on quaternary structure for recognition of proteins may also be relevant to other protein-protein interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00000579
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  • 8
    Electronic Resource
    Electronic Resource
    Velocity-temperature correlation in strongly heated channel flow (1995)
    Springer
    Experiments in fluids 18 (1995), S. 454-461 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Velocity-temperature correlations in a strongly heated channel flow were investigated experimentally by a LDV and a resistance thermometer. The wall heat flux is varied up to 50,000 W/m2 with reference mean-velocity of 15 m/s, and then, the wall temperature reaches up to 1,000 K. The results show that the ejection fluid motion is intensified by the strong heating near the wall increasing the turbulent heat flux from the wall. The intensification of ejection motion balances the destruction of turbulent heat flux. Then, the overall turbulent heat transfer does not change clearly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208469
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  • 9
    Electronic Resource
    Electronic Resource
    Velocity statistics along the stagnation line of an axi-symmetric stagnating turbulent flow (1997)
    Springer
    Experiments in fluids 22 (1997), S. 473-481 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  Velocity statistics along the stagnation line of an axi-symmetric wall stagnating turbulent flow are studied experimentally. A low turbulence, uniform air flow from a nozzle type air supply with an exit diameter of 50 mm stagnates at a wall located 50 mm downstream. A flow velocity is set to 3 m/s, 10 mm downstream from the exit of the air supply. Instantaneous values of streamwise and radial velocities are measured by laser-Doppler velocimetry. The turbulence level in the air flow is changed by use of turbulence generator. When the turbulence generator is not installed in the air supply, the mean velocity profile in the streamwise direction fits well with that of a laminar viscous flow with the rms value of velocity fluctuations low near the wall. With the turbulence generator installed, a significant turbulence structure appears near the wall. When the wall is approached, the rms value of velocity fluctuations in the streamwise direction decreases monotonically while the profile of the rms value in the radial direction reaches a maximum near the wall. The increase in the rms value of velocity fluctuations in the radial direction near the wall is attributed to the bi-modal histogram of the fluctuating velocity in the radial direction. Near the wall, the instantaneous stagnation streamline fluctuates and the probability of the mean location of the stagnation point reaches a maximum not at the stagnation line but on a circle around the stagnation line, resulting in the bi-modal histogram. Turbulence statistics, the rms value of velocity fluctuation and the turbulent kinetic energy, can be normalized successfully by similarity parameters based on the strain rate and the reference turbulent kinetic energy introduced by Champion and Libby.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480050074
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  • 10
    Electronic Resource
    Electronic Resource
    Turbulence structure in an annuli with strongly heated inner cylinder (1999)
    Springer
    Experiments in fluids 27 (1999), S. 137-144 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This structure of turbulent flow in an annulus with strong inner cylinder wall heating has been studied in terms of velocity and temperature with wall temperatures up to 707 °C and a Reynolds number of 48,000. With increase in wall heating, the turbulence very close to the wall was suppressed due to an increase in the kinematic viscosity. In the inner region, the intermittent mixing became intensive and the turbulent intensity increased whereas, in the outer region, the turbulence was suppressed since intermittent mixing was no longer effective. The results show that the thermal structure can be considered in terms of a passive scalar for wall temperatures less then around 200 °C, except in the leading region of the heated area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480050338
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