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  • 1
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A number of chemically modified hemoglobin preparations have been proposed for use as an emergency resuscitation fluid. The purpose for forming these hemoglobin derivatives is to decrease the oxygen binding (i.e., to increase the P50) and to increase the intravascular retention time. These goals have been met with various degrees of success by using the reaction with pyridoxyl 5-phosphate to raise the P50, followed by the addition of glutaraldehyde to increase circulating half-life by polymerization.1,2 Other derivatives have been formed with polyethylene glycol,3,4 bis-(3,5-dibromosalicyl) fumarate,5,6 glycolaldehyde,7 and 2-nor-2-formylpyridoxal 5-phosphate,8,9 as well as with other compounds. All these derivatives introduce a foreign molecule into the hemoglobin, which may not always be desirable. Recently Tharp and Day10 used cyanogen to form intersubunit amide cross-links in hemoglobin without the incorporation of cyanogen. This approach is attractive if the appropriate functional properties can be attained. Takeda et al.11 showed that equimolar concentrations of amino acids and disuccinimidyloxalate could form peptide bonds in high yield. We report the characteristics of the hemoglobin molecule modified by internal covalent amide bonds, which may be a suitable candidate for a resuscitation fluid.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Pyridoxylated adult human hemoglobin (HbAo) was prepared using a one molar equivalent of pyridoxal 5-phosphate (PLP) per heme and reduced with either NaCNBH3 or NaBH4. A separate sample was pyridoxylated and passed through a mixed-bed ion exchange column without reduction. All three preparations had a P50 of 29 ± 2 torr and a cooperativity of n = 2.4 ± 0.1. These preparations, in both the oxy and deoxy forms, were then treated with 7 equivalents of glutaraldehyde per tetramer at pH 6.8 at 4°C and at room temperature. The polymerization invariably reduced the P50 to 18 ± 2 torr with Hill coefficients of less than 2. These solutions, with or without further reduction using NaCNBH3, all retained the PLP in differing amounts (2-3 moles/tetramer). Methemoglobin concentrations were increased during the polymerization reaction. The normal pyridoxylation procedure, using sodium borohydride reduction, resulted in a number of different molecular species. Polymerization with glutaraldehyde caused a further proliferation of molecular species that could not be separated by anion exchange chromatography or by isoelectric focusing. The extent of polymerization, estimated by gel exclusion chromatography and SDS polyacrylamide gel electrophoresis, was from 40 to 50%. Analysis of the reverse phase chromatograms, which separate the heme and the α- and β-chains, showed extensive polymerization and distribution of the radioactively labeled PLP on the protein for all preparations. All of the polymerized and pyridoxylated samples were unstable, and showed different chromatographic patterns after storage at 4°C for 1 month. Attempts to stabilize these preparations by further reduction with NaCNBH3 gave products with a lower P50 and lower cooperativity. When the reactions were conducted with a purified HbAo, heterogeneity was somewhat decreased compared to the normally used stroma-free hemoglobin, but a large number of molecular species were still formed.
    Additional Material: 8 Ill.
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  • 3
    Publication Date: 2016-10-19
    Description: Using the data from over 8000 wells augmented by seismic and thermal response information, a comparison of McMurray Formation (Cretaceous) and Grosmont C member (Devonian) thermal recovery reservoirs of northeastern Alberta is provided along with a discussion of reservoir performance to date. Fluvial-estuarine McMurray Formation reservoirs perform best where bitumen-charged homogeneous lenticular sandstones at least 20 metres thick are found. These deposits are relatively rare as the formation is characterized by endemic heterogeneity mainly in the form of inclined heterolithic stratification (IHS). Most of the best McMurray steam-assisted gravity drainage (SAGD) reservoirs appear to be currently on-line and produce approximately 113 000 m 3 /day of bitumen from fourteen projects. Platform carbonate Grosmont C successions are blanket deposits 32–35 metres thick, with bitumen columns typically 15–24 metres thick, and are characterized by consistent reservoir properties facilitated by pervasive multi-scale fracturing. Although no reserves have yet to be assigned to Alberta’s bitumen-bearing carbonates by the province, recent pilot results derived from cyclic steam stimulation (CSS) operations suggest that Grosmont C reservoir performance could ultimately prove to be competitive with superior McMurray SAGD reservoirs. Under current technological and economic conditions, McMurray SAGD reservoirs appear incapable of providing the 15.9 billion m 3 of in-situ bitumen reserves (59% of Canada’s total oil reserves) ascribed to this formation by the province of Alberta as only circa 6 billion m 3 of oil-in place appears to reside within optimal reservoirs (i.e. those reservoirs at least 20 metres thick with average porosity and oil saturation values of 33% and 80%, respectively). Barring future technological breakthroughs and, or, economic improvements, future commercial development of both the Grosmont C and other carbonate reservoirs might be needed to make up for some of the potential reserve shortfall associated with McMurray Formation SAGD reservoirs.
    Print ISSN: 0007-4802
    Electronic ISSN: 0007-4802
    Topics: Geosciences
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  • 4
    Publication Date: 2015-09-24
    Description: Despite geophysics is being used increasingly, it is still unclear how and when the integration of geophysical data improves the construction and predictive capability of groundwater models. Therefore, this paper presents a newly developed HYdrogeophysical TEst-Bench (HYTEB) which is a collection of geological, groundwater and geophysical modeling and inversion software wrapped to make a platform for generation and consideration of multi-modal data for objective hydrologic analysis. It is intentionally flexible to allow for simple or sophisticated treatments of geophysical responses, hydrologic processes, parameterization, and inversion approaches. It can also be used to discover potential errors that can be introduced through petrophysical models and approaches to correlating geophysical and hydrologic parameters. With HYTEB we study alternative uses of electromagnetic (EM) data for groundwater modeling in a hydrogeological environment consisting of various types of glacial deposits with typical hydraulic conductivities and electrical resistivities covering impermeable bedrock with low resistivity. It is investigated to what extent groundwater model calibration and, often more importantly, model predictions can be improved by including in the calibration process electrical resistivity estimates obtained from TEM data. In all calibration cases, the hydraulic conductivity field is highly parameterized and the estimation is stabilized by regularization. For purely hydrologic inversion (HI, only using hydrologic data) we used Tikhonov regularization combined with singular value decomposition. For joint hydrogeophysical inversion (JHI) and sequential hydrogeophysical inversion (SHI) the resistivity estimates from TEM are used together with a petrophysical relationship to formulate the regularization term. In all cases, the regularization stabilizes the inversion, but neither the HI nor the JHI objective function could be minimized uniquely. SHI or JHI with regularization based on the use of TEM data produced estimated hydraulic conductivity fields that bear more resemblance to the reference fields than when using HI with Tikhonov regularization. However, for the studied system the resistivities estimated by SHI or JHI must be used with caution as estimators of hydraulic conductivity or as regularization means for subsequent hydrological inversion. Much of the lack of value of the geophysical data arises from a mistaken faith in the power of the petrophysical model in combination with geophysical data of low sensitivity, thereby propagating geophysical estimation errors into the hydrologic model parameters. With respect to reducing model prediction error, it depends on the type of prediction whether it has value to include geophysical data in the model calibration. It is found that all calibrated models are good predictors of hydraulic head. When the stress situation is changed from that of the hydrologic calibration data, then all models make biased predictions of head change. All calibrated models turn out to be a very poor predictor of the pumping well's recharge area and groundwater age. The reason for this is that distributed recharge is parameterized as depending on estimated hydraulic conductivity of the upper model layer which tends to be underestimated. Another important insight from the HYTEB analysis is thus that either recharge should be parameterized and estimated in a different way, or other types of data should be added to better constrain the recharge estimates.
    Print ISSN: 1812-2108
    Electronic ISSN: 1812-2116
    Topics: Geography , Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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  • 5
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