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  • Articles  (122)
  • Springer  (122)
  • 1995-1999  (121)
  • 1955-1959  (1)
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  • Articles  (122)
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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of segregation and aneuploidy in two reciprocal translocation carriers, t(3;9)(q26.2;q32) and t(3;9)(p25;q32), by triple-color fluorescence in situ hybridization (1999)
    Springer
    Human genetics 〈Berlin〉 105 (1999), S. 428-436 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Meiotic segregation patterns of chromosomes 3 and 9 were analyzed in sperm of two translocation carriers (t(3;9)(q26.2;q32) and t(3;9)(p25;q32)) by triple-color fluorescent in situ hybridization (FISH) with a telomeric DNA probe in addition to two centromeric probes. The frequencies of each sperm product resulting from alternate or adjacent I, adjacent II and 3:1 segregation in a t(3;9)(q26.2;q32) translocation carrier were 88.35%, 5.44% and 5.94%, respectively. On the other hand, the frequencies of each sperm product in a t(3;9)(p25;q32) translocation carrier were 89.23%, 6.02% and 4.48%, respectively. Of all the sperm products, the frequency of normal or chromosomally balanced sperm in a t(3;9)(q26.2;q32) and a t(3;9)(p25;q32) were 52.49% and 47.25%, respectively. The frequencies of each sperm product resulting from various segregations were different between both carriers and significantly deviated from the expected frequencies. Additional dual-color and triple-color FISH were performed to analyze aneuploidy rates for chromosomes 12, 17, 18, X and Y in order to detect any interchromosomal effect; no evidence of an interchromosomal effect was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004399900161
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  • 2
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    Analysis of segregation and aneuploidy in two reciprocal translocation carriers, t(3;9)(q26.2;q32) and t(3;9)(p25;q32), by triple-color fluorescence in situ hybridization (1999)
    Springer
    Details
    Publication Date: 1999-11-01
    Print ISSN: 0340-6717
    Electronic ISSN: 1432-1203
    Topics: Biology , Medicine
    Published by Springer
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  • 3
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    Analysis of segregation and aneuploidy in two reciprocal translocation carriers, t(3;9)(q26.2;q32) and t(3;9)(p25;q32), by triple-color fluorescence in situ hybridization (1999)
    Springer
    Details
    Publication Date: 1999-11-15
    Print ISSN: 0340-6717
    Electronic ISSN: 1432-1203
    Topics: Biology , Medicine
    Published by Springer
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    PAPER CURRENT
  • 4
    Electronic Resource
    Electronic Resource
    Thioredoxin h is one of the major proteins in rice phloem sap (1995)
    Springer
    Planta 195 (1995), S. 456-463 
    Details
    ISSN: 1432-2048
    Keywords: Oryza (phloem proteins) ; Phloem sap ; protein ; Sieve tube ; Thioredoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sieve tubes play important roles in the transfer of nutrients as well as signals. Hundreds of proteins were found in pure phloem sap collected from rice (Oryza sativa L. cv. Kantou) plants through the cut ends of insect stylets. These proteins may be involved in nutrient transfer and signal transduction. To characterize the nature of these proteins, the partial amino-acid sequence of a 13kDa protein, named RPP13-1, that was abundant in the pure phloem sap was determined. A cDNA clone of 687 bp, containing an open reading frame of 122 amino acids, was isolated using corresponding oligonucleotides as a probe. The deduced amino-acid sequence was very similar to that of the ubiquitous thiol redox protein, thioredoxin. The consensus sequences of thioredoxins are highly conserved. No putative signal peptide was identified. Antiserum against wheat thioredoxin h cross-reacted with RPP13-1 in the phloem sap of rice plants. RPP131 produced in Escherichia coli was reactive to antiserum against wheat thioredoxin h. Both E. coli-produced RPP13-1 and the phloem sap proteins catalyzed the reduction of the disulfide bonds of insulin in the presence of dithiothreitol. These results indicate that an active thioredoxin is a major protein translocating in rice sieve tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202605
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of Seven Major Evolutionary Lineages in Cyanobacteria Based on the 16S rRNA Gene Sequence Analysis with New Sequences of Five Marine Synechococcus Strains (1999)
    Springer
    Journal of molecular evolution 48 (1999), S. 723-739 
    Details
    ISSN: 1432-1432
    Keywords: Key words: Cyanobacteria —Synechococcus— Phylogeny — Ribosomal RNA — Maximum-likelihood method — Neighbor-joining method — Rate homo- and heterogeneous models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Although molecular phylogenetic studies of cyanobacteria on the basis of the 16S rRNA gene sequence have been reported, the topologies were unstable, especially in the inner branchings. Our analysis of 16S rRNA gene phylogeny by the maximum-likelihood and neighbor-joining methods combined with rate homogeneous and heterogeneous models revealed seven major evolutionary lineages of the cyanobacteria, including prochlorophycean organisms. These seven lineages are always stable on any combination of these methods and models, fundamentally corresponding to phylogenetic relationships based on other genes, e.g., psbA, rbcL, rnpB, rpoC, and tufA. Moreover, although known genotypic and phenotypic characters sometimes appear paralleled in independent lineages, many characters are not contradictory within each group. Therefore we propose seven evolutionary groups as a working hypothesis for successive taxonomic reconstruction. New 16S rRNA sequences of five unicellular cyanobacterial strains, PCC 7001, PCC 7003, PCC 73109, PCC 7117, and PCC 7335 of Synechococcus sp., were determined in this study. Although all these strains have been assigned to ``marine clusters B and C,'' they were separated into three lineages. This suggests that the organisms classified in the genus Synechococcus evolved diversely and should be reclassified in several independent taxonomic units. Moreover, Synechococcus strains and filamentous cyanobacteria make a monophyletic group supported by a comparatively high statistical confidence value (80 to 100%) in each of the two independent lineages; therefore, these monophylies probably reflect the convergent evolution of a multicellular organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006517
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  • 6
    Electronic Resource
    Electronic Resource
    An age-related decrease in the concentration of insulin-like growth factor binding protein-5 in human cortical bone (1995)
    Springer
    Calcified tissue international 57 (1995), S. 206-212 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The skeletal contents of insulin-like growth factor-2 (IGF-II), insulin-like growth factor binding protein-5 (IGFBP-5), and insulin-like growth factor binding protein-3 (IGFBP-3) were determined in duplicate samples of human femoral cortical bone obtained from 64 subjects (44 males and 20 females) between the ages of 20 and 64 years. The results of these quantitative measurements revealed an age-related decrease in the femoral cortical content of IGFBP-5 (r=-0.272, P=0.031) in the total population. Although the femoral cortical content of IGF-II did not show a similar decrease with age, it could be correlated to the femoral cortical content of IGFBP-5 (r=0.442, P〈0.001). In constrast, the femoral cortical content of IGFBP-3 did not decrease with age and could not be correlated to the femoral cortical contents of either IGFBP-5 or IGF-II. Comparisons of these results with previous measurements of insulin-like growth factor-1 (IGF-I) and transforming growth factor-β (TGF-β), in extracts of the same bones, showed significant cross-correlations between the femoral cortical contents of each of these growth factors and the femoral cortical contents of IGFBP-5 (r=0.625 for IGF-I versus IGFBP-5, r=0.554 for TGF-β versus IGFBP-5, P〈0.001 for each) but not IGFBP-3. Together, these data indicate average net losses of 60% and 29% of the femoral cortical contents of IGF-I and IGFBP-5, respectively, and apparent net losses (i.e., nonsignificant decreases) of 21% and 25% of the femoral cortical contents of IGF-II and TGF-β, respectively, between the third and the sixth decades (i.e., decreases from young adult values of 75.1 pmol/g of bone for IGF-I, 124.7 pmol/g of bone for IGF-II, 0.71 pmol/g of bone for TGF-β, 115.6 pmol/g of bone for IGFBP-5, and 26.2 pmol/g of bone for IGFBP-3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310260
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  • 7
    Electronic Resource
    Electronic Resource
    Osteogenic Protein-1 Stimulates mRNA Levels of BMP-6 and Decreases mRNA Levels of BMP-2 and -4 in Human Osteosarcoma Cells (1997)
    Springer
    Calcified tissue international 60 (1997), S. 297 -301 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Bone morphogenetic protein — Osteogenic protein-1 — Transforming growth factor-β— Osteoblasts — Growth factors.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bone morphogenetic proteins (BMPs) are novel growth and differentiation factors that act on mesenchymal stem cells to initiate new bone formation in vivo and promote the growth and differentiation of cells in the osteoblastic lineage. In the present study, we examined the effects of recombinant human osteogenic protein-1 (also known as BMP-7) on the expression of related members of the BMP family using SaOS-2 and U2-OS, two human osteosarcoma cell strains. Evaluation of BMP-2, -4, and -6 mRNA expression indicates that OP-1 stimulated the mRNA levels of BMP-6 in both SaOS-2 cells (threefold) and U2-OS cells (fivefold) after 24 hours of treatment, while decreasing the mRNA levels of BMP-4 in SaOS-2 cells (80%) and BMP-2 and BMP-4 in U2-OS cells by 50% and 72%, respectively. BMP-2 mRNA expression, as examined by Northern blot analysis, was below detectable limits in SaOS-2 cultures. These results demonstrate that OP-1 modulates the mRNA expression of related members of the BMP family, suggesting a possible mode of action of OP-1 on the growth and differentiation of cells in the osteoblastic lineage in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900232
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  • 8
    Electronic Resource
    Electronic Resource
    Vitamin B12 amplifies circadian phase shifts induced by a light pulse in rats (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 691-694 
    Details
    ISSN: 1420-9071
    Keywords: Circadian rhythm ; intracerebroventricular infusion ; light pulse ; phase shift ; rats ; vitamin B12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Vitamin B12 (VB12) is a putative modulator of the human circadian clock, improving entrainability to the 24 h light-dark cycle. The present study was intended to elucidate the mechanism of VB12 action in an animal model. In male rats free-running under constant dim illumination, a single light pulse of 50–1000 lux for 20 min given at circadian time (CT) 20 induced a 0.28 to 1.08 h phase advance and at CT 14 induced a 0.54 to 2.10 h phase delay. A 3 h intracerebroventricular (icv) infusion of 30 nmol VB12 starting 2 h prior to a 20 min 200 lux light pulse significantly amplified phase shifts in comparison with saline-treated or untreated controls. The mean phase advance (1.13 h) was 1.8-fold greater than that of saline-infused controls, whereas the mean phase delay (2.28 h) was 2.9-fold greater. These values were comparable to the maximal phase shifts caused by 1000 lux light pulses in untreated rats. Since the same VB12 treatment alone had failed to induce a phase shift in a previous experiment, these results indicate that VB12 strongly enhanced light pulse-induced phase shifts and thus augmented the entrainability of the circadian clock to light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01925576
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  • 9
    Electronic Resource
    Electronic Resource
    Alkaloidal oviposition stimulants for a danaid butterfly,Ideopsis similis L., from a host plant,Tylophora tanakae (Asclepiadaceae) (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 753-756 
    Details
    ISSN: 1420-9071
    Keywords: Oviposition stimulants ; Ideopsis similis ; Danaidae ; Tylophora tanakae ; Asclepiadaceae ; (+)-isotylocrebrine ; (−)-7-demethyltylophorine ; alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Chemicals releasing oviposition by an Asclepiadaceae feeder,Ideopsis similis, were identified from a host plant,Tylophora tanakae. A strong positive response was evoked by a methanolic extract of the plant, which proved to contain multiple stimulants. A mixture of two phenanthroindolizidine alkaloids, (+)-isotylocrebrine and (−)-7-demethyltylophorine, isolated from organic fractions, elicited significant ovipositional responses from females.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01941275
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  • 10
    Electronic Resource
    Electronic Resource
    Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 72-76 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Culture conditions of Schizochytrium limacinum SR21 for the purpose of microbial docosahexaenoic acid (DHA) production were investigated. The strain SR21 showed a wide tolerance to salinity; that is, the optimum salinity was between 50% and 200% that of sea water. Monosaccharides (glucose and fructose) and glycerol supported good cell growth and DHA yield. Di- and polysaccharides, oleic acid, and linseed oil gave low DHA yields. A high content of DHA (more than 30% of total fatty acids) was obtained from culture on glucose, fructose, and glycerol, and also the strain had simple polyunsaturated fatty acid profiles. The major polyunsaturated fatty acids other than DHA were n-6 docosapentaenoic acid only, and the contents of icosapentaenoic acid and arachidonic acid were less than 1%. Using corn steep liquor as a nitrogen source, a high total fatty acid content was obtained. The total fatty acid content in the dry cell weight increased as the concentration of the nitrogen source decreased, reached more than 50%. An increase in carbon source concentration led to a high DHA yield. A maximum DHA yield of more than 4 g/l was obtained in both glucose and glycerol media at 9% and 12% respectively. S. limacinum SR21 was thought to be a promising resource for microbial DHA production yielding a good level of productivity as well as a simple polyunsaturated fatty acid profile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051139
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