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  • Life Sciences (General)  (14)
  • taxonomy  (10)
  • 2000-2004  (24)
  • 1
    Electronic Resource
    Electronic Resource
    Secretory cavities and pellucid dots in leaflets ofLonchocarpus (Leguminosae, Papilionoideae, Millettieae) (2000)
    Springer
    Plant systematics and evolution 221 (2000), S. 61-68 
    Details
    ISSN: 1615-6110
    Keywords: Leguminosae ; Papilionoideae ; Lonchocarpus ; leaflets ; secretory cavities ; false secretory cavities ; pellucid dots ; glands ; anatomy ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of leaflet secretory cavities in 23 Brazilian species ofLonchocarpus was surveyed anatomically and compared with data from external morphology (presence of pellucid dots) to evaluate their taxonomic significance. This study revealed three cases: presence of secretory cavities associated with pellucid dots, pellucid dots corresponding to false secretory cavities, and absence of pellucid dots and secretory cavities. These results indicate that in this genus the “glandular pellucid dots” cited in morphological descriptions do not always correspond to secretory cavities, and that their nature must always be confirmed by histological sections. Secretory cavities have systematic significance at subgeneric taxonomic level for the genusLonchocarpus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01086380
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular variation in Galdieria sulphuraria (Galdieri) Merola and its bearing on taxonomy (2000)
    Springer
    Hydrobiologia 433 (2000), S. 145-151 
    Details
    ISSN: 1573-5117
    Keywords: Cyanidium caldarium ; Cyanidioschyzon merolae ; Galdieria sulphuraria ; rbcL ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanidium caldarium, Cyanidioschyzon merolae and Galdieria sulphuraria are three unicellular algae characteristic, of acid thermal environments. Recently, on the basis of morphological characters, three new species of Galdieria (G. partita, G. daedala, G. maxima ) isolated from acid-thermal springs in Russia have been instituted. A selected region of rbcL and the sequence of the intergenic spacer between the rbcL and rbcS have been amplified and sequenced from different Galdieria species and strains, in order to define molecular relationship among these interesting algae. The obtained cladogram shows that Cyanidium caldarium and Cyanidioschyzon merolae form a sister group which, in turn, is in a sister group relationship with Galdieria. This last genus is divided in two clades, one of which includes G. sulphuraria accessions from Naples (Italy), California, and Yellowstone and the other one includes G. sulphuraria accessions from Java (Indonesia) and from the Russian species. These results support the status of the genus Galdieria and suggest that G. daedala, G. maxima and G. partita are three very similar strains of G. sulphuraria; the rbcL variation within Galdieria accessions has a pattern which is broadly connected to the geographial distribution. The data obtained from the intergenic rbcL-rbcS spacer partly confirm those from the rbcL analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004035224715
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  • 3
    Electronic Resource
    Electronic Resource
    Re-examination of some species of the genus Geotrichum Link: Fr (2000)
    Springer
    Antonie van Leeuwenhoek 77 (2000), S. 71-81 
    Details
    ISSN: 1572-9699
    Keywords: DNA heterogeneity ; genome comparison ; Geotrichum ; taxonomy ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nutritional physiology and the growth rate of thirty-four strains representing species of Geotrichum without known teleomorph states were examined. From twenty-seven strains the mol% G+C were calculated from the DNA melting curves. The first derivatives of the melting curves of seven strains, including the type strain of Geotrichum clavatum, demonstrated the presence of two peaks, 12% away from each other; the remaining strains showed only a single broad peak. DNA homology values among strains of the former group were high, indicating their conspecificity. The strains of the latter group could be subdivided into six DNA homology groups, four of which could be identified with recognized species and two may represent novel taxa. A combined key of Geotrichum and its teleomorph states Galactomyces and Dipodascus is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002030518989
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  • 4
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    Effects of heavy ions on visual function and electrophysiology of rodents: the ALTEA-MICE project (2004)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: ALTEA-MICE will supplement the ALTEA project on astronauts and provide information on the functional visual impairment possibly induced by heavy ions during prolonged operations in microgravity. Goals of ALTEA-MICE are: (1) to investigate the effects of heavy ions on the visual system of normal and mutant mice with retinal defects; (2) to define reliable experimental conditions for space research; and (3) to develop animal models to study the physiological consequences of space travels on humans. Remotely controlled mouse setup, applied electrophysiological recording methods, remote particle monitoring, and experimental procedures were developed and tested. The project has proved feasible under laboratory-controlled conditions comparable in important aspects to those of astronauts' exposure to particle in space. Experiments are performed at the Brookhaven National Laboratories [BNL] (Upton, NY, USA) and the Gesellschaft fur Schwerionenforschung mbH [GSI]/Biophysik (Darmstadt, FRG) to identify possible electrophysiological changes and/or activation of protective mechanisms in response to pulsed radiation. Offline data analyses are in progress and observations are still anecdotal. Electrophysiological changes after pulsed radiation are within the limits of spontaneous variability under anesthesia, with only indirect evidence of possible retinal/cortical responses. Immunostaining showed changes (e.g. increased expression of FGF2 protein in the outer nuclear layer) suggesting a retinal stress reaction to high-energy particles of potential relevance in space. c2004 COSPAR. Published by Elsevier Ltd. All rights reserved.
    Keywords: Life Sciences (General)
    Type: Advances in space research : the official journal of the Committee on Space Research (COSPAR); Volume 33; 8; 1347-51
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  • 5
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    The ALTEA/ALTEINO projects: studying functional effects of microgravity and cosmic radiation (2004)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: The ALTEA project investigates the risks of functional brain damage induced by particle radiation in space. A modular facility (the ALTEA facility) is being implemented and will be operated in the International Space Station (ISS) to record electrophysiological and behavioral descriptors of brain function and to monitor their time dynamics and correlation with particles and space environment. The focus of the program will be on abnormal visual perceptions (often reported as "light flashes" by astronauts) and the impact on retinal and brain visual structures of particle in microgravity conditions. The facility will be made available to the international scientific community for human neurophysiological, electrophysiological and psychophysics experiments, studies on particle fluxes, and dosimetry. A precursor of ALTEA (the 'Alteino' project) helps set the experimental baseline for the ALTEA experiments, while providing novel information on the radiation environment onboard the ISS and on the brain electrophysiology of the astronauts during orbital flights. Alteino was flown to the ISS on the Soyuz TM34 as part of mission Marco Polo. Controlled ground experiments using mice and accelerator beams complete the experimental strategy of ALTEA. We present here the status of progress of the ALTEA project and preliminary results of the Alteino study on brain dynamics, particle fluxes and abnormal visual perceptions. c2004 COSPAR. Published by Elsevier Ltd. All rights reserved.
    Keywords: Life Sciences (General)
    Type: Advances in space research : the official journal of the Committee on Space Research (COSPAR); Volume 33; 8; 1352-7
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  • 6
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    Effect of spaceflight on the maximal shortening velocity, morphology, and enzyme profile of fast- and slow-twitch skeletal muscle fibers in rhesus monkeys (2000)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: Weightlessness has been shown to cause limb muscle wasting and a reduced peak force and power in the antigravity soleus muscle. Despite a reduced peak power, Caiozzo et al. observed an increased maximal shortening velocity in the rat soleus muscle following a 14-day space flight. The major purpose of the present investigation was to determine if weightlessness induced an elevated velocity in the antigravity slow type I fibers of the rhesus monkey (Macaca mulatta), as well as to establish a cellular mechanism for the effect. Spaceflight or models of weightlessness have been shown to increase glucose uptake, elevate muscle glycogen content, and increase fatigability of the soleus muscle. The latter appears to be in part caused by a reduced ability of the slow oxidative fibers to oxidize fats. A second goal of this study was to establish the extent to which weightlessness altered the substrate profile and glycolytic and oxidative enzyme capacity of individual slow- and fast-twitch fibers.
    Keywords: Life Sciences (General)
    Type: Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology (ISSN 1077-9248); Volume 7; 1; S37-8
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  • 7
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    Effect of spaceflight on the isotonic contractile properties of single skeletal muscle fibers in the rhesus monkey (2000)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: Experiments from both Cosmos and Space Shuttle missions have shown weightlessness to result in a rapid decline in the mass and force of rat hindlimb extensor muscles. Additionally, despite an increased maximal shortening velocity, peak power was reduced in rat soleus muscle post-flight. In humans, declines in voluntary peak isometric ankle extensor torque ranging from 15-40% have been reported following long- and short-term spaceflight and prolonged bed rest. Complete understanding of the cellular events responsible for the fiber atrophy and the decline in force, as well as the development of effective countermeasures, will require detailed knowledge of how the physiological and biochemical processes of muscle function are altered by spaceflight. The specific purpose of this investigation was to determine the extent to which the isotonic contractile properties of the slow- and fast-twitch fiber types of the soleus and gastrocnemius muscles of rhesus monkeys (Macaca mulatta) were altered by a 14-day spaceflight.
    Keywords: Life Sciences (General)
    Type: Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology (ISSN 1077-9248); Volume 7; 1; S53-4
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  • 8
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    Autotrophy of green non-sulphur bacteria in hot spring microbial mats: biological explanations for isotopically heavy organic carbon in the geological record (2000)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: Inferences about the evidence of life recorded in organic compounds within the Earth's ancient rocks have depended on 13C contents low enough to be characteristic of biological debris produced by the well-known CO2 fixation pathway, the Calvin cycle. 'Atypically' high values have been attributed to isotopic alteration of sedimentary organic carbon by thermal metamorphism. We examined the possibility that organic carbon characterized by a relatively high 13C content could have arisen biologically from recently discovered autotrophic pathways. We focused on the green non-sulphur bacterium Chloroflexus aurantiacus that uses the 3-hydroxypropionate pathway for inorganic carbon fixation and is geologically significant as it forms modern mat communities analogous to stromatolites. Organic matter in mats constructed by Chloroflexus spp. alone had relatively high 13C contents (-14.9%) and lipids diagnostic of Chloroflexus that were also isotopically heavy (-8.9% to -18.5%). Organic matter in mats constructed by Chloroflexus in conjunction with cyanobacteria had a more typical Calvin cycle signature (-23.5%). However, lipids diagnostic of Chloroflexus were isotopically enriched (-15.1% to -24.1%) relative to lipids typical of cyanobacteria (-33.9% to -36.3%). This suggests that, in mats formed by both cyanobacteria and Chloroflexus, autotrophy must have a greater effect on Chloroflexus carbon metabolism than the photoheterotrophic consumption of cyanobacterial photosynthate. Chloroflexus cell components were also selectively preserved. Hence, Chloroflexus autotrophy and selective preservation of its products constitute one purely biological mechanism by which isotopically heavy organic carbon could have been introduced into important Precambrian geological features.
    Keywords: Life Sciences (General)
    Type: Environmental microbiology (ISSN 1462-2912); Volume 2; 4; 428-35
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  • 9
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    Sleep, performance, circadian rhythms, and light-dark cycles during two space shuttle flights (2001)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: Sleep, circadian rhythm, and neurobehavioral performance measures were obtained in five astronauts before, during, and after 16-day or 10-day space missions. In space, scheduled rest-activity cycles were 20-35 min shorter than 24 h. Light-dark cycles were highly variable on the flight deck, and daytime illuminances in other compartments of the spacecraft were very low (5.0-79.4 lx). In space, the amplitude of the body temperature rhythm was reduced and the circadian rhythm of urinary cortisol appeared misaligned relative to the imposed non-24-h sleep-wake schedule. Neurobehavioral performance decrements were observed. Sleep duration, assessed by questionnaires and actigraphy, was only approximately 6.5 h/day. Subjective sleep quality diminished. Polysomnography revealed more wakefulness and less slow-wave sleep during the final third of sleep episodes. Administration of melatonin (0.3 mg) on alternate nights did not improve sleep. After return to earth, rapid eye movement (REM) sleep was markedly increased. Crewmembers on these flights experienced circadian rhythm disturbances, sleep loss, decrements in neurobehavioral performance, and postflight changes in REM sleep.
    Keywords: Life Sciences (General)
    Type: American journal of physiology. Regulatory, integrative and comparative physiology (ISSN 0363-6119); Volume 281; 5; R1647-64
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  • 10
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    Short-term adaptation of the VOR: non-retinal-slip error signals and saccade substitution (2003)
    In:  Other Sources
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    Publication Date: 2019-07-13
    Description: We studied short-term (30 min) adaptation of the vestibulo-ocular reflex (VOR) in five normal humans using a "position error" stimulus without retinal image motion. Both before and after adaptation a velocity gain (peak slow-phase eye velocity/peak head velocity) and a position gain (total eye movement during chair rotation/amplitude of chair motion) were measured in darkness using search coils. The vestibular stimulus was a brief ( approximately 700 ms), 15 degrees chair rotation in darkness (peak velocity 43 degrees /s). To elicit adaptation, a straight-ahead fixation target disappeared during chair movement and when the chair stopped the target reappeared at a new location in front of the subject for gain-decrease (x0) adaptation, or 10 degrees opposite to chair motion for gain-increase (x1.67) adaptation. This position-error stimulus was effective at inducing VOR adaptation, though for gain-increase adaptation the primary strategy was to substitute augmenting saccades during rotation while for gain-decrease adaptation both corrective saccades and a decrease in slow-phase velocity occurred. Finally, the presence of the position-error signal alone, at the end of head rotation, without any attempt to fix upon it, was not sufficient to induce adaptation. Adaptation did occur, however, if the subject did make a saccade to the target after head rotation, or even if the subject paid attention to the new location of the target without actually looking at it.
    Keywords: Life Sciences (General)
    Type: Annals of the New York Academy of Sciences (ISSN 0077-8923); 1004; 94-110
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