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  • Brownian motion analysis  (1)
  • supercoiling  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 106 (1999), S. 57-62 
    ISSN: 1573-6857
    Keywords: DNA elasticity ; DNA torsional rigidity ; supercoiling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The torsional modulus C of DNA is determined from the difference between the work of stretching a single overwound molecule and the work done in stretching one underwound by the same number of turns. The value obtained C/kBT=86±10 nm is within the range (75±25 nm) estimated by more indirect methods.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of statistical physics 93 (1998), S. 647-672 
    ISSN: 1572-9613
    Keywords: DNA micromanipulation ; DNA supercoiling ; DNA structural transitions ; Brownian motion analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract New micromanipulation techniques now enable physicists and biologists to study the behavior of single biomolecules such as DNA. In particular, it is possible to measure the elastic response of individual DNA molecules to changes in the double helix's supercoiling. The force versus extension diagram for torsionally relaxed DNA is continuous and allows one to evaluate the persistence length of the polymer. When the molecule is supercoiled, however, stretching leads to the buildup of torsional stress in the double helix's axis. When the twist energy thus generated increases beyond a critical value, the molecule is locally destabilized and changes conformation. This structural transition occurs at stretching forces which can be exerted in vivo by molecular motors and at degrees of supercoiling found in the cell, and may have implications for DNA structure and function within the nucleus.
    Type of Medium: Electronic Resource
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