ISSN:
1573-4986
Keywords:
Heparan sulfate
;
glycosaminoglycan synthesis
;
sulfation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Microsomal preparations from Englebreth-Holm-Swarm mouse sarcoma were incubated with UDP-N-acetyl[3H] glucosamine and UDP-[14C]glucuronic acid to form proteoglycan containing [3H,14C]glycosaminoglycan with equimolar amounts of [3H]glucosamine and [14C]glucuronic acid. The labelled glycosaminoglycan was totally resistant to degradation by testicular hyaluronidase, but could be degraded readily by a crudeFlavobacter heparinum enzyme preparation which is capable of degrading heparin and heparan sulfate. Chromatography of the [3H,14C]glycosaminoglycan on DEAE-cellulose provided a pattern with three peaks: the first appearing before hyaluronic acid, the second and largest appearing at the site of hyaluronic acid, and a third appearing slightly beyond hyaluronic acid but before a standard of chondroitin sulfate. When 3′-phosphoadenosine 5′-phosphosulfate was also included in the reaction mixture, a change appeared in the [3H,14C]glycosaminoglycan so that chromatography on DEAE-cellulose presented a pattern with a significant amount of material which cochromatographed in the area where heparan sulfate would be found. There was no material that co-chromatographed with the more highly sulfated substance, heparin. This indicates that the microsomal preparation from the Englebreth-Holm-Swarm sarcoma is capable of producing a heparan sulfate-like molecule and is controlled in its sulfation of precursors so that heparin is not formed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01875413
Permalink
