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  • 1
    ISSN: 1423-0445
    Keywords: sex pheromone ; courtship ; behaviour ; (z)-11-pentacosene ; fruit fly ; Diptera ; Drosophilidae ; Drosophila virilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A crude cuticular extract from 3450 virgin 9–13 day old female fruit flies(Drosophila virilis), was subjected to chromatography accompanied by bioassay for sex pheromone activity. After three chromatographic steps, fractions containing active monoenes and dienes were obtained. Chemical analysis by infrared absorption, gas liquid chromatography and gas chromatography/mass spectrometry of the active fraction indicated that active monoenes were comprised chiefly of (Z)-11-pentacosene (abbreviated (Z)-11-C25:1), (Z)-13-C27:1’, (Z)-13- and (Z)-14-C29:1. Synthetic monoenes were made, and only (Z)-11-C25:1 elicited good courtship behaviour in maleD. virilis. Therefore it was concluded that (Z)-11-C25:1 was a major sex pheromone. A total of 16.2±1.32 µg of cuticular hydrocarbons was isolated from 10 day old females, including 5.9±0.56 µg of (Z)-11-C25:1. An additive effect was suggested from the higher observed courtship response when using a mixture of active dienes with the active monoene.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Drosophila pallidosa ; Diptera ; Drosophilidae ; sex pheromone ; cuticular hydrocarbon ; (Z,Z)-5,27-tritriacontadiene ; courtship behavior ; fruit fly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A crude cuticular extract from both sexes of 3660 fruit flies (Drosophila pallidosa) was subjected to SiO2 and AgNO3/SiO2 column chromatography, accompanied by bioassay for the sex pheromone activity. After three chromatographic steps, the active fraction was obtained. The main component of the active fraction was determined to be (Z,Z)-5,27-tritriacontadiene [(Z,Z)-5,27-C33:2, on the basis of gas-liquid chromatographic analysis, chemical derivatization and gas chromatography-mass spectrometry. Synthetic (Z,Z)-5,27-C33:2 at 5 female equivalents (FE) elicited a clear courtship response with a high courtship index amongD. pallidosa males. Therefore it was concluded that (Z,Z)-5,27-C33:2 was a major sex pheromone component in this species.
    Type of Medium: Electronic Resource
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