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  • 1
    Electronic Resource
    Electronic Resource
    SERCA pump isoform expression in endothelium of veins and arteries: Every endothelium is not the same (2000)
    Springer
    Molecular and cellular biochemistry 203 (2000), S. 11-15 
    Details
    ISSN: 1573-4919
    Keywords: coronary artery ; coronary vein ; aorta ; vena cava ; sarcoplasmic reticulum ; endoplasmic reticulum ; ATPase ; calcium pump ; smooth muscle ; ischemia-reperfusion ; signal transduction ; transport ; cardiovascular ; cDNA ; mRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Endothelium from rat aorta expresses sarco/endoplasmic reticulum Ca2+(SERCA) pump gene SERCA3 where as the smooth muscle expresses SERCA2. This has led to the postulate that vascular endothelium expresses SERCA3. To test this postulate, we examined the SERCA2 and SERCA3 mRNA expression in endothelium and smooth muscle dissected from coronary artery, coronary vein, aorta and vena cava of pig. Smooth muscle from all arteries and veins expressed only the SERCA2 mRNA. Endothelium from coronary artery, coronary vein and aorta expressed both SERCA2 and SERCA3 mRNA but the endothelium from vena cava did not express SERCA3 mRNA although it expressed SERCA2. These observations support the postulate that vascular endothelium expresses SERCA3 but the affirmation is equivocal because vena cava endothelium does not express SERCA3. (Mol Cell Biochem 000: 000-000, 1999)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007093516593
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  • 2
    Electronic Resource
    Electronic Resource
    Angiotensin II contractions in coronary artery (1994)
    Springer
    Molecular and cellular biochemistry 135 (1994), S. 11-19 
    Details
    ISSN: 1573-4919
    Keywords: cardiac ; fluorescence ; cyclopiazonic acid ; thapsigargin ; sarcoplasmic reticulum ; smooth muscle ; vascular
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Pig coronary artery rings denuded of endothelium contract to the vasoactive hormone angiotensin II (Ang II). The nature of Ang II receptors and their Ca2+-pool utilization were examined for contraction of the artery rings and for increase in ultracellular [Ca2+] ([Ca2+]i) in smooth muscle cells cultured from them. Ang II contracted the arteries (EC50=7±4 nM) but with a lower maximal force (1.4±0.25 N/g tissue) than the contraction with 60 mM K+ (6.11±0.63 N/g tissue). In the cultured cells it caused a transient increase in [Ca2+]i with an EC50 value of 11±4 nM. The cells bound Ang II with a dissociation constant (Kd) of 7±2 nM. Based on the effects of the Ang II antagonists saralasin, DuPont 753, dithiothreitol and PD123319, the Ang II receptors responsible for contraction, increase in [Ca2+]i and Ang II binding to coronary artery smooth muscle were of type AT1. The contraction to Ang II was abolished by EGTA but not by nitrendipine. The sarcoplasmic Ca2+ pump inhibitors cyclopiazonic acid (10 μM CPA) and thapsigargin (1 μM) produced contractions of 4.35±0.73 and 2.07±0.54 N/g, respectively. Ang II contractions in the control arteries were nearly abolished upon pretreatment with CPA and thapsigargin. CPA and thapsigargin induced contractions were abolished by exposure to EGTA for 1 h but short exposure of the cells to EGTA only modulated the CPA or thapsigargin induced increase in [Ca2+]i; Ang II induced increase in [Ca2+]i was not inhibited by 1 μM nitrendipine but was reduced significantly by a 30–60 sec exposure to EGTA. CPA and thapsigargin caused an increase in [Ca2+]i even after 30–200 sec exposure to EGTA. Ang II when added after CPA or thapsigargin did not cause a further increase in [Ca2+]i but when added before them it caused an increase in [Ca2+]i and reduced the increase caused by subsequent addition of CPA or thapsigargin. These data are consistent with the concept that Ang II utilizes an intracellular Ca2+-pool which is a small component of the CPA or thapsigargin sensitive Ca2+-pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00925957
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of peroxide on contractility of coronary artery rings of different sizes (1999)
    Springer
    Molecular and cellular biochemistry 194 (1999), S. 159-164 
    Details
    ISSN: 1573-4919
    Keywords: free radicals ; ischemia-reperfusion ; sarcoplasmic reticulum ; Ca2+-Mg2+-ATPase ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Reactive oxygen species (ROS, free radicals) produced during cardiac ischemia and reperfusion can damage the contractile functions of arteries. The sarcoplasmic reticulum (SR) Ca2+ pump in coronary artery smooth muscle is very sensitive to ROS. Here we show that contractions of de-endothelialized rings from porcine left coronary artery produced by the hormone Angiotensin II and by the SR Ca2+ pump inhibitors cyclopiazonic acid and thapsigargin correlate negatively with the tissue weight. In contrast, the contractions due to membrane depolarization by high KCl correlate positively. Peroxide also produces a small contraction which correlates negatively with the tissue weight. When artery rings are treated with peroxide and washed, their ability to contract with Angiotensin II, cyclopiazonic acid and thapsigargin decreases. Thus, the SR Ca2+ pump may play a more important role in the contractility of the smaller segments of the coronary artery than in the larger segments. These results are consistent with the hypothesis that ROS which damage the SR Ca2+ pump affect the contractile function of the distal segments more adversely than of the proximal segments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006902603056
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