ISSN:
1573-5079
Keywords:
CO2/O2specificity factor
;
mutagenesis
;
rubisco small subunit
;
tau
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Seven unique substitutions have been introduced by site-directed mutagenesis into the first conserved region of the small subunit of ribulose bisphosphate carboxylase/oxygenase from Anacystis nidulans 6301. After expression of each large, altered-small subunit gene tandem in Escherichia coli, two substitutions in the small subunit tyr17→asp17 (Y17D) and arg10→gly10 (R10G) result in little or no carboxylase activity. For the latter substitution, no L8S8enzyme complex could be detected suggesting that this mutation prevents assembly. Mutant enzymes containing the following substitutions R11G, T14A, S16A, Y17D and P19A have CO2/O2specificity factors (τ values) of 40, 35, 18, 39 and 44, respectively, compared with that of 44 for wild-type recombinant enzyme whereas P20A has full carboxylase activity and a τ value of 55.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005967106993
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