ISSN:
1573-4943
Keywords:
α-sarcin
;
mitogillin
;
restrictocin
;
circular dichroism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract α-Sarcin, restrictocin, and mitogillin, three antitumor proteins, have been compared in terms of chemical composition as well as secondary and tertiary structure. The amino acid composition of the three polypeptides showed that restrictocin and mitogillin are essentially identical, α-Sarcin is also very similar to the other two proteins, although it lacks one methionine in its amino acid composition. Peptide maps of restrictocin and mitogillin coincide, except that one additional peptide is present in the mitogillin fingerprint. Although the α-sarcin map is different from the other two fingerprints, seven tryptic peptides with identical chromatographic and electrophoretic properties as well as amino acid composition were identified. The secondary and tertiary structures of mitogillin and restrictocin are identical from circular dichroism and difference spectroscopy studies. α-Sarcin has slightly different spectroscopic properties than the other two proteins. From these studies, the three proteins could be considered homologous polypeptides.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01025357
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