ISSN:
1573-5044
Keywords:
Acryl-coenzyme A transferase
;
biosynthesis
;
coenzyme A thioester
;
Datura
;
pseudotropine
;
transformed root cultures
;
tropane alkaloids
;
tropine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transformed root cultures of Datura stramonium and of related species contain both aliphatic and aromatic tropane esters. It has been shown that these esters are produced by the action of several acyl transferases that transfer the acidic moiety to tropan-3α-ol (tropine) or tropan-3β-ol (pseudotropine) from various acyl-coenzyme A thioesters. The presence of these enzymes has been examined in a range of tropane-alkaloid-producing and non-tropane-alkaloid-producing species. Activities that esterify tropine appear to be confined to species that accumulate tropane alkaloids, whereas a number of species that do not accumulate tropane alkaloids possess some ability to esterify pseudotropine. The present state of knowledge of these enzymes is reviewed. One of these activities, tigloyl-Coenzyme A:pseudotropine acyl transferase, has been purified to near homogeneity and the properties of this enzyme are summarized.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033883
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