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  • 1
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; pre-harvest sprouting ; germination ; α-amylase enzyme ; gibberellic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sensitivity to GA in non-Gai genome winter wheat (Triticum aestivum L.) cultivars was investigated to determine magnitude of variation of the trait, its association with other traits, and effects of geographical location of production. α-Amylase enzyme activity was measured before and after treatment with gibberellic acid in 18 cultivars grown at one location and in five cultivars grown at six locations. Dye-labeled starch and agar-starch media procedures were used for the first and second experiments, respectively. α-Amylase activity differed significantly among the 18 cultivars after germination in water, germination in GA, and in absolute and relative sensitivity to GA. Cultivars that reacted similarly to GA had some common ancestors in their pedigrees, and α-amylase activity and sensitivity to GA were significantly negatively associated with seed weight. α-Amylase activity differed between tall and semi-dwarf cultivars in the second experiment, but not in the first experiment. The magnitude of variation in α-amylase activity and its relationship to preharvest sprouting susceptibility of the cultivars suggested that the trait can be modified to improve seed dormancy. Significant interactions between cultivar responses to GA and geographical location of grain production suggested that selection should be carried out in several environments.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Triticum destivum ; wheat ; pre-harvest sprouting ; inhibitors ; embryo ; germination ; gibberellic acid ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seed dormancy in wheat (Triticum aestivum L. em Thell) is important for minimizing pre-harvest sprouting. To facilitate breeding cultivars that tolerate pre-harvest sprouting conditions, we assessed mode and magnitude of variation of seed dormancy among genotypes and investigated involvement of endogenous water-soluble inhibitor(s) in seed germination. Embryo bio-assays established that water-soluble inhibitor was ubiquitous among the wheat cultivars studied and did not diminish in quantity during after-ripening. Germination response of embryos was decreased by endogenous inhibitor, but the effect markedly declined as embryos aged at room temperature. Variation in dormancy among cultivars was primarily caused by differential response of their embryos to endogenous inhibitor. Gibberellic acid counteracted the initial inhibitory effect of endogenous inhibitor on germination but not the subsequent inhibitory effect on seedling growth. We concluded that pre-harvest sprouting resistance involves multiple factors, particularly embryo receptivity to endogenous inhibitor, and that variation in inhibitor quantity is not solely responsible for genotypic differences in susceptibility to pre-harvest sprouting. The possibility of additional approaches to breeding for pre-harvest sprouting resistance is indicated.
    Type of Medium: Electronic Resource
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