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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 101 (1987), S. 211-221 
    ISSN: 1573-5036
    Schlagwort(e): aeration ; calcium ; cotton ; flooding ; magnesium ; manganese ; phosphorus ; potassium ; sodium chloride ; waterlogging
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A field experiment was conducted to investigate the effects of intermittent waterlogging on the nutrient status of cotton (Gossypium hirsutum cv. Deltapine 61). The crop was grown in a sloping plot of soil in which a gradient of water-table depth ranging from 0.04m above to 0.60m below the soil surface was established during two periods of waterlogging in mid summer and early autumn. The first waterlogging lasted 8 days; the second lasted 16 days. Dry matter increases were less for severely waterlogged plants than for plants with well-aerated root systems during the first flooding, but the increases were similar during the second. Waterlogging impaired uptake of most nutrients by young plants in the first flooding, but had much less effect on nutrient uptake by older plants in the second. Waterlogging consistently reduced concentrations of P and K in the petioles and laminae of young fully-expanded leaves, and severely waterlogged plants were deficient in these nutrients by the end of the first flooding. Mn did not accumulate to toxic levels in waterlogged plants. During each flooding, waterlogged plants gained in total content of all nutrients studied, but the gains of each nutrient, except for Na, were proportionally smaller than for well-aerated plants. Fluxes of K-, Cl- and HPO4- ions in xylem sap exuded from stumps of detopped plants which had been waterlogged were lower than those from plants with well-aerated root systems. Seed cotton yields and concentrations of nutrients in mature bolls were not affected by the two periods of waterlogging. It is concluded that although intermittent waterlogging induced nutrient stress in cotton plants, especially for P and K in young plants before flowering, they recovered with no detrimental effect upon yield.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 102 (1987), S. 161-170 
    ISSN: 1573-5036
    Schlagwort(e): aeration ; flooding ; Gossypium hirsutum L. ; root zone ; soil oxygen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Surface-irrigated cotton (Gossypium hirsutum L.) grown on slowly draining clay soil is subjected to short-term periods of waterlogging at each irrigation which generally results in reduced productivity. The sequence of above- and below-ground plant responses to transient waterlogging and the role of N availability in modifying the immediate responses were studied. Lysimeters of Marah clay loam (a Natrustalf) were instrumented to monitor soil and plant responses to a 7-day waterlogging event beginning 67 days after sowing. Cotton (‘Deltapine 61’) plants (8 per lysimeter) were grown with two levels of added N (300 kg ha−1 and 30 kg ha−1) and two irrigation treatments (flooded and control). Measured soil-O2 levels decreased rapidly upon surface flooding because water displaced air and root zone respiration consumed O2. The rate of O2 consumption was 2.7 times greater in the high-N treatment than the low-N treatment. This difference was associated with a 1.8 fold difference in numbers of observed roots. Root growth was only slightly affected by flooding. Leaf growth decreased by 28%, foliage temperature increased 2.3% and apparent photosynthesis decreased by 16%. It is suggested that flooding reduced photosynthetic activity within 2 days while other stress symptoms became apparent after about 6 days. Although this stress was reflected in a trend for decreased plant productivity, the effect of flooding on boll dry mass at harvest was not significant at the level of replication used. The single waterlogging did not cause yield reductions comparable to those observed elsewhere when several waterlogging events were imposed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2016-01-08
    Beschreibung: The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across 〉 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1–D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial β-tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α); and vi) the second largest subunit of RNA-polymerase II (partial RPB2, section 5–6). Their PCR efficiencies were compared with novel candidate primers corresponding to: i) the fungal-specific translation elongation factor 3 (TEF3); ii) a small ribosomal protein necessary for t-RNA docking; iii) the 60S L10 (L1) RP; iv) DNA topoisomerase I (TOPI); v) phosphoglycerate kinase (PGK); vi) hypothetical protein LNS2; and vii) alternative sections of TEF1α. Results showed that several gene sections are accessible to universal primers (or primers universal for phyla) yielding a single PCR-product. Barcode gap and multi-dimensional scaling analyses revealed that some of the tested candidate markers have universal properties providing adequate infra- and inter-specific variation that make them attractive barcodes for species identification. Among these gene sections, a novel high fidelity primer pair for TEF1α, already widely used as a phylogenetic marker in mycology, has potential as a supplementary DNA barcode with superior resolution to ITS. Both TOPI and PGK show promise for the Ascomycota, while TOPI and LNS2 are attractive for the Pucciniomycotina, for which universal primers for ribosomal subunits often fail.
    Schlagwort(e): DNA barcoding ; ITS supplement ; molecular taxonomy ; phylogeny ; species identification ; universal primers
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: Article / Letter to the editor
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2024-02-03
    Beschreibung: The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Ampli\xef\xac\x81cation ef\xef\xac\x81ciencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across 〉\xe2\x80\xaf1\xe2\x80\xaf500 species (1\xe2\x80\xaf931 strains or specimens) and the outcomes of almost twenty thousand (19\xe2\x80\xaf577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1\xe2\x80\x93D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial \xce\xb2-tubulin II (TUB2); iv) \xce\xb3-actin (ACT); v) translation elongation factor 1-\xce\xb1 (TEF1\xce\xb1); and vi) the second largest subunit of RNA-polymerase II (partial RPB2, section 5\xe2\x80\x936). Their PCR ef\xef\xac\x81ciencies were compared with novel candidate primers corresponding to: i) the fungal-speci\xef\xac\x81c translation elongation factor 3 (TEF3); ii) a small ribosomal protein necessary for t-RNA docking; iii) the 60S L10 (L1) RP; iv) DNA topoisomerase I (TOPI); v) phosphoglycerate kinase (PGK); vi) hypothetical protein LNS2; and vii) alternative sections of TEF1\xce\xb1. Results showed that several gene sections are accessible to universal primers (or primers universal for phyla) yielding a single PCR-product. Barcode gap and multi-dimensional scaling analyses revealed that some of the tested candidate markers have universal properties providing adequate infra- and inter-speci\xef\xac\x81c variation that make them attractive barcodes for species identi\xef\xac\x81cation. Among these gene sections, a novel high \xef\xac\x81delity primer pair for TEF1\xce\xb1, already widely used as a phylogenetic marker in mycology, has potential as a supplementary DNA barcode with superior resolution to ITS. Both TOPI and PGK show promise for the Ascomycota, while TOPI and LNS2 are attractive for the Pucciniomycotina, for which universal primers for ribosomal subunits often fail.
    Schlagwort(e): DNA barcoding ; ITS supplement ; molecular taxonomy ; phylogeny ; species identi\xef\xac\x81cation ; universal primers
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
    Format: application/pdf
    Standort Signatur Erwartet Verfügbarkeit
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