ISSN:
1573-9368
Keywords:
cosuppression
;
gene silencing
;
Nicotiana benthamiana
;
pathogen‐derived resistance
;
TuMV
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV‐ESC8) was cloned and sequenced. Comparisons of the 867‐nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7–89.3% nucleotide identity and 92.4–95.5% amino acid identity. The CP gene was cloned into a plant expression vector and transformed into Nicotiana benthamiana plants via Agrobacterium tumefaciens‐mediated leaf disk transformation. Progeny from R0 lines was screened for resistance to TuMV‐ESC8. Five of 29 tested lines showed TuMV protection in more than 50% of their progeny. Interestingly, some of the resistant plants transformed with the CP gene of TuMV displayed mild mosaicism in the new growing leaves at the later stages of evaluation; but these mosaic symptoms disappeared when the leaves were fully expanded. Collective data from steady‐state RNA analysis and nuclear run‐on assay of a line showed that the resistance was RNA‐mediated through the post‐transcriptional gene silencing mechanism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008915007271
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