ISSN:
1432-072X
Keywords:
Pseudomonas nautica 617
;
Marine bacterium
;
Denitrification
;
Heptadecane
;
oxygen
;
Nitrate and nitrite reductases
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The denitrifying marine bacterium, Pseudomonas nautica 617, can grow on lactate aerobically or anaerobically in presence of nitrate with generation times of 1.5 and 3 h respectively. The growth on heptadecane occurs only in presence of oxygen whatever its concentration with a genrration time of 8.5 h. The influence of oxygen, carbon sources (lactate or heptadecane) and nitrate was examined on O2, NO3 -, NO2 - consumption, on nitrate and nitrite reductases activities, on cell yields, and on the ratio of CO2 produced per unit of biomass. Pseudomonas nautica metabolizes hydrocarbons under denitrifying conditions in the presence of oxygen. Nitrate and nitrite are used during growth on lactate and heptadecane up to oxygen concentrations corresponding to 50 and 30% of air-saturation, respectively. When growth on n-alkane was not oxygen-limited (above 50% of air-saturation) the catabolism decreases in favour of carbon incorporation into the cell. Nitrate and nitrite reductases were strongly inhibited after 20% of airsaturation in the presence of lactate as growth substrate. With n-alkane, only the nitrate reductase activity was greatly reduced.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00276775
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