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  • oncogene regulation  (2)
  • Leporinus  (1)
  • Medaka  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Analysis of an esterase linked to a locus involved in the regulation of the melanoma oncogene and isolation of polymorphic marker sequences inXiphophorus (1991)
    Springer
    Biochemical genetics 29 (1991), S. 509-524 
    Details
    ISSN: 1573-4927
    Keywords: Xiphophorus ; melanoma ; oncogene regulation ; esterase ; molecular marker sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Melanoma formation inXiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by theTu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecular identification of this regulatory locus (R) requires a precise physical map of the chromosomal region. Therefore we studied esterase isozymes inXiphophorus, two of which have been previously reported to be linked to locusR. We confirm thatES1 is a distant marker forR (approx. 30cM), and contrary to earlier studies, we show that this isozyme is present in all species of the genus and at similar activity levels in all organs tested.ES4, which has also been reported to be linked toR, was found to be a misclassification of liverES1. In an attempt to identify markers that bridge the large distance betweenES1 andR, we have generated DNA probes which are highly polymorphic. They will be useful in finding landmarks on a physical map of theR-containing chromosomal region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554126
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of an esterase linked to a locus involved in the regulation of the melanoma oncogene and isolation of polymorphic marker sequences inXiphophorus (1991)
    Springer
    Biochemical genetics 29 (1991), S. 509-524 
    Details
    ISSN: 1573-4927
    Keywords: Xiphophorus ; melanoma ; oncogene regulation ; esterase ; molecular marker sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Melanoma formation inXiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by theTu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecular identification of this regulatory locus (R) requires a precise physical map of the chromosomal region. Therefore we studied esterase isozymes inXiphophorus, two of which have been previously reported to be linked to locusR. We confirm thatES1 is a distant marker forR (approx. 30cM), and contrary to earlier studies, we show that this isozyme is present in all species of the genus and at similar activity levels in all organs tested.ES4, which has also been reported to be linked toR, was found to be a misclassification of liverES1. In an attempt to identify markers that bridge the large distance betweenES1 andR, we have generated DNA probes which are highly polymorphic. They will be useful in finding landmarks on a physical map of theR-containing chromosomal region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426867
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  • 3
    Electronic Resource
    Electronic Resource
    Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes) (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 129-140 
    Details
    ISSN: 1617-4623
    Keywords: Medaka ; Gene transfer ; Transient expression ; DNA fate ; Fish developmental biology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Species of small fish are becoming useful tools for studies on vertebrate development. We have investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporal and spatial expression patterns of bacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3- and 9-month-old adult fish; it was found in high molecular weight form as well as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273596
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  • 4
    Electronic Resource
    Electronic Resource
    Cytogenetics of the Genus Leporinus (Pisces, Anostomidae). II. Molecular Cytogenetics, Organization and Evolutionary Conservation of a Chromosome-Specific Satellite DNA from Leporinus obtusidens (1997)
    Springer
    Chromosome research 5 (1997), S. 325-331 
    Details
    ISSN: 1573-6849
    Keywords: evolution ; fish molecular cytogenetics ; Leporinus ; satellite DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A chromosome-specific satellite DNA from the South American fish species Leporinus obtusidens has been isolated and characterized. Sequence analysis and Southern hybridization studies indicate that the cloned 483-bp fragment is 60% AT rich and appears to comprise two diverged monomers. A highly variable low-copy number polymorphism was detected and, thus, this satellite DNA may serve as a valuable genetic marker. Using a Southern blot approach, the cloned satellite DNA cross-hybridized strongly to the DNA of Leporinus elongatus but failed to detect homologous sequences in the genomes of other closely related Leporinus species and higher vertebrates. Using fluorescence in situ hybridization to mitotic metaphase spreads of L. obtusidens and L. elongatus, this satellite DNA was located to the (peri)centromeric region of one single chromosome pair in both species. As the cloned satellite DNA sequence clearly evolved along a chromosomal lineage and is highly variable, it may serve as a very useful marker in further genetic, molecular and cytogenetic studies of the genus Leporinus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/B:CHRO.0000038763.52875.48
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