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  • 1
    Electronic Resource
    Electronic Resource
    Hydroxylamine metabolism in Pseudomonas PB16: involvement of a novel hydroxylamine oxidoreductase (1997)
    Springer
    Antonie van Leeuwenhoek 71 (1997), S. 69-74 
    Details
    ISSN: 1572-9699
    Keywords: hydroxylamine ; nitrification ; aerobic denitrification ; Pseudomonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pseudomonas strain PB16, a Gram-negative heterotrophic nitrifying bacterium closely related to Pseudomonas azalaica on the basis of 16 S rDNA analysis, was able to use hydroxylamine as an additional energy source during growth in acetate limited chemostat cultures giving an increased biomass yield. In aerobically growing cells of Pseudomonas PB16 only 50% of supplemented hydroxylamine could be recovered as nitrite. In addition to nitrite, N2O could be detected in the chemostat off-gas, indicating combined heterotrophic nitrification and aerobic denitrification. The maximum specific hydroxylamine oxidizing activity observed was 450 nmol per min per mg dry weight, with a Ks of approximately 40 µm. Upon addition of hydroxylamine to the medium, Pseudomonas PB16 induced a soluble 132 KDa dimeric hydroxylamine oxidoreductase. The enzyme had a pH optimum of 9, and did not contain spectroscopic features typical for cytochromes, which is in contrast to hydroxylamine oxidoreductases fou nd in autotrophic bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000145617904
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  • 2
    Electronic Resource
    Electronic Resource
    Novel principles in the microbial conversion of nitrogen compounds (1997)
    Springer
    Antonie van Leeuwenhoek 71 (1997), S. 75-93 
    Details
    ISSN: 1572-9699
    Keywords: hydroxylamine ; anaerobic ammonium oxidation ; nitrification ; denitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Some aspects of inorganic nitrogen conversion by microorganisms like N2O emission and hydroxylamine metabolism studied by Beijerinck and Kluyver, founders of the Delft School of Microbiology, are still actual today. In the Kluyver Laboratory for Biotechnology, microbial conversion of nitrogen compounds is still a central research theme. In recent years a range of new microbial processes and process technological applications have been studied. This paper gives a review of these developments including, aerobic denitrification, anaerobic ammonium oxidation, heterotrophic nitrification, and formation of intermediates (NO2-, NO, N2O), as well as the way these processes are controlled at the genetic and enzyme level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1000150219937
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    Paper (German National Licenses)
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