ISSN:
0006-3525
Keywords:
solid-phase synthesis
;
dendrimer
;
hyperbranched polymers
;
polyamidoamine
;
bead loading amplification
;
multiple antigen peptide system
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Dendrimers are highly ordered, hyperbranched polymers with potential for a whole range of chemical applications. Solution-phase synthesis of dendrimers is often challenging, requiring long reaction times and nontrivial purification; solid-phase methodology, on the other hand, enables reactions to be driven to completion by using a large excess of reagents with trivial purification. In this paper we show that polyamidoamine dendrimers may be synthesized conveniently and efficiently on a solid support, and that these molecules are of good homogeneity as characterized by 1H- and 13C-nmr and electrospray mass spectrometry. These solid-phase dendrimers were used as a bead loading amplification tool in the synthesis of a hexapeptide library (Xaa-Gly-Gly-Phe-Leu-Lys) to allow single bead analysis as well as allowing the efficient synthesis of two dendrimer conjugates (Leu-enkephalin-Lys and Chlorambucil). This demonstrated that peptides and small drug molecules can be grown directly onto the dendrimer or onto a linker attached to the dendrimer periphery, enhancing general dendrimer utility. © 1999 John Wiley & Sons, Inc. Biopoly 47: 381-396, 1998
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
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