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  • L-Phenylalanine-copper and L-proline-copper complex used as chiral reagents in the mobile phase  (1)
  • moisture content  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biologia plantarum 42 (1999), S. 499-504 
    ISSN: 1573-8264
    Keywords: plant growth regulators ; moisture content ; acorn size ; developmental stages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To induce somatic embryogenesis in Quercus robur L. immature zygotic embryos at different developmental stages were collected in weekly intervals from June until September in three consecutive years from four open pollinated trees at two Vienna sites. Acorns were surface sterilised and cultured firstly on P24 medium with 5μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 μM 6-benzylaminopurine (BAP) or on hormone-free P24 medium and secondly on P24 medium with 0.9 μM BAP. The formation of white-yellow globular structures of somatic embryos started during the fourth week after the induction treatment. High induction frequencies of 30 - 80 % were achieved on 2,4-D/BAP medium, whereas rates on hormone-free medium were below 20 %. The initiation of somatic embryogenesis was favoured in the heartshaped and early cotyledonary stage of the zygotic embryo in all three years and lasted until the acorns reached maximum size in August.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 269-272 
    ISSN: 0935-6304
    Keywords: Liquid chromatography, HPLC ; Simple analytical procedure for separation of enantiomers ; L-Phenylalanine-copper and L-proline-copper complex used as chiral reagents in the mobile phase ; α-Methyldopa, tryptophan, thyroid hormones ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Simple procedures are presented for separating the enantiomers of α-methyldopa, 5-hydroxytryptophan, tryptophan, triiodothyronine and thyroxine, which require neither special sorbents nor difficult-to-obtain or unstable reagents.The method for α-methyldopa, 5-hydroxytryptophan and tryptophan is based on the use of L-phenylalanine copper complex as the chiral constituent of the mobile phase; LiChrosorb® RP-18 serves as the stationary phase. The procedure for triiodothyronine and thyroxine is grounded on the L-proline copper complex as the chiral reagent and LiChrosorb® Si 60 as the stationary phase.In all observed cases, the D-enantiomer is eluted prior to the respective L-enantiomer. Chirality inversion of the mobile phase (application of the D-phenylalanine copper complex) reverses the order of elution; a racemic eluent (DL-phenylalanine copper complex) leads to no separation. In addition to the enantiomers of α-amino acids, the enantiomers of α-hydroxy acids (mandelic acid) can be separated.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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