ISSN:
1573-5044
Keywords:
liana
;
micropropagation
;
naphthylisoquinoline alkaloids
;
tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae), a West African liana producing naphthylisoquinoline alkaloids, was successfully raised from seeds in vitro. Clonal propagation was best achieved growing nodal stem segments on 1/5 Linsmaier and Skoog medium with full strength organics and supplemented with 0.02 μM thidiazuron, 4.44 μM 6-benzylaminopurine and 0.05 μM 1-naphthaleneacetic acid. Detached axillary shoots were grown on Anderson's Rhododendron medium devoid of phytohormones and rooted within one month when dipped in 4.92 μM indole-3-butyric acid. Rooted plants became acclimatized to nonsterile greenhouse conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006201711390
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