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  • 1
    ISSN: 1573-5028
    Keywords: heat shock ; heat shock cDNAs ; maize ; small heat shock proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heat-shocked maize seedlings (cv. Oh43) synthesize a characteristic set of heat-shock proteins (hsps) which include an 18 kDa family containing at least six major isoelectric variants. A cDNA library was constructed from poly(A)+ RNAs isolated from the radicles of heat-shocked maize seedlings and screened with a DNA fragment from the theoretical open reading frame of a putative Black Mexican Sweet maize hsp 18 genomic clone. Two clones, cMHSP18-3 and cMHSP18-9, were isolated, and the RNA transcripts generated from them were translated into proteins which immunoreact with antibodies directed against the maize 18 kDa hsps and exhibit the same electrophoretic characteristics as two different members of the 18 kDa hsp family. Nucleotide sequence analyses of the cDNAs in these clones reveal that their 5′ and 3′ untranslated regions exhibit 33–34% identity and that their protein encoding regions share 93% identity. The deduced amino acid sequences of these clones show 90% identity, and the apparent molecular masses and isoelectric points of these proteins agree with those established for two different 18 kDa hsps, numbered 3 and 6. This report substantiates that at least two of the 18 kDa hsps in maize are products of different but related genes. Moreover, it establishes that transcripts for these proteins accumulate during heat shock and that both their nucleotide and deduced amino acid sequences share extensive similarities with the class VI small hsps in soybean and with transcripts expressed during meiosis in Lilium.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5028
    Keywords: carotenoid biosynthesis ; endosperm ; gene ; maize ; phytoene desaturase ; regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To study regulation of the plastid-localized maize carotenoid biosynthetic pathway, a cDNA encoding phytoene desaturase (PDS) was isolated and characterized. The DNA sequence of the maize Pds cDNA was determined and compared with available dicot Pds genes. The deduced PDS protein, estimated at 64.1 kDa (unprocessed), had a dinucleotide binding domain and conserved regions characteristic of other carotene desaturases. Alignment of available PDS sequences from distantly related organisms suggests that Pds has potential as a phylogenetic tool. By use of heterologous complementation in Escherichia coli, maize PDS was shown to catalyze two desaturation steps converting phytoene to ζ-carotene. RFLP (restriction fragment length polymorphism) mapping was used to place Pds on chromosome 1S near viviparous5 (vp5), and RT-PCR (reverse-transcriptase polymerase chain reaction) analysis indicated reduced Pds transcript in vp5 mutant relative to normal endosperm. Other phytoene-accumulating mutant endosperms, vp2 and white3 (w3), showed no difference in Pds transcript accumulation as compared with normal endosperm counterparts. RT-PCR analysis of Pds transcript accumulation in developing endosperm showed Pds was constitutively expressed. Therefore, endosperm carotenogenesis is not regulated by increasing the level of Pds transcripts.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0192-253X
    Keywords: Heat shock protein ; maize ; mi-crosporogenesis ; gametogenesis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The small (18-kDa) heat shock proteins (hsps) of maize are encoded by a complex multigene family. In a previous report, we described the genetic information from cDNAs encoding two different members of the family. In this communication, we report the isolation and characterization of cDNA and genomic clones encoding information for a third member of this hsp family (c/gMHSP18-1). DNA fragments containing nucleotide sequences common to, or specific for, each of these characterized 18-kDa genes were prepared and used as probes to assess the expression of these genes during microsporogenesis and development of the gametophyte in an inbred line of maize (Oh43). Our results demonstrate (1) that mRNA transcripts encoding the 18-kDa hsps are expressed and/or accumulate during microsporogenesis, and (2) that genes encoding two of the characterized 18-kDa hsps are expressed and/or accumulate independently, in a stage-specific manner during microsporogenesis. These observations imply that the stage-specific expression of particular 18-kDa hsp genes results from gene-specific regulation during microsporogenesis and gametophyte development rather than from an overall activation of the heat shock or stress response. © 1993Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 4
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    Arizona Board of Regents | Marine Biological Laboratory Archives (Woods Hole, Mass.)
    In:  Viktor Hamburger collection, Box 2, Folder 60, Paul Weiss, Marine Biological Laboratory Archives
    Publication Date: 2023-01-12
    Description: Invitation to dinner.
    Description: Typewritten carbon copy of original letter.
    Description: 1-page letter
    Description: Correspondence
    Keywords: People
    Repository Name: Woods Hole Open Access Server
    Language: English
    Type: Text
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  • 5
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    Marine Biological Laboratory Archives (Woods Hole, Mass.)
    In:  Viktor Hamburger collection, Box 2, Folder 46, Marine Biological Laboratory Archives
    Publication Date: 2023-01-12
    Description: General updates
    Description: Correspondence
    Keywords: People
    Repository Name: Woods Hole Open Access Server
    Language: English
    Type: Text
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  • 6
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    Marine Biological Laboratory Archives (Woods Hole, Mass.)
    In:  Viktor Hamburger collection, Box 2, Folder 46, Marine Biological Laboratory Archives
    Publication Date: 2023-01-12
    Description: News on travels, microscope, anatomy course
    Description: Correspondence
    Keywords: People ; Tools
    Repository Name: Woods Hole Open Access Server
    Language: English
    Type: Text
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  • 7
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    Marine Biological Laboratory Archives (Woods Hole, Mass.)
    In:  Viktor Hamburger collection, Box 2, Folder 46, Marine Biological Laboratory Archives
    Publication Date: 2023-01-12
    Description: Update on zoology course. Personal updates
    Description: Correspondence
    Keywords: People
    Repository Name: Woods Hole Open Access Server
    Language: English
    Type: Text
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  • 8
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    Marine Biological Laboratory Archives (Woods Hole, Mass.)
    In:  Viktor Hamburger collection, Box 2, Folder 46, Marine Biological Laboratory Archives
    Publication Date: 2023-01-12
    Description: General updates
    Description: Correspondence
    Keywords: People
    Repository Name: Woods Hole Open Access Server
    Language: English
    Type: Text
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  • 9
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    Marine Biological Laboratory Archives (Woods Hole, Mass.)
    In:  Viktor Hamburger collection, Box 2, Folder 46, Marine Biological Laboratory Archives
    Publication Date: 2023-01-12
    Description: Travel and personnel changes
    Description: Correspondence
    Keywords: People
    Repository Name: Woods Hole Open Access Server
    Language: English
    Type: Text
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  • 10
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    Marine Biological Laboratory Archives (Woods Hole, Mass.)
    In:  Viktor Hamburger collection, Box 2, Folder 46, Marine Biological Laboratory Archives
    Publication Date: 2023-01-12
    Description: Personnel discussions
    Description: Correspondence
    Keywords: People
    Repository Name: Woods Hole Open Access Server
    Language: English
    Type: Text
    Format: image/tiff
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