ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Isozyme patterns in callus cultures and in plants regenerated from calli ofCereus peruvianus (Cactaceae) (1994)
    Springer
    Biochemical genetics 32 (1994), S. 237-247 
    Details
    ISSN: 1573-4927
    Keywords: isozymes ; callus cultures ; cactaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electrophoretic patterns for isocitrate dehydrogenase (IDH; EC 1.1.1.42), acid phosphatase (ACP; EC 3.1.3.2), peroxidase (PER; EC 1.11.1.7), and esterase (EST; EC 3.1.1.1) isozymes were determined inCereus peruvianus tissues and used as markers of genetic uniformity of calli and of the plants regenerated from callus cultures. One IDH, six ACP, six PER, and six EST isozymes were induced in cultured callus tissues in medium containing three 2,4-dichlorophenoxyacetic acid and kinetin combinations. Four ACP, two PER, and three EST isozymes were still present in all regenerated plantsin vitro and therefore can be used as markers of theC. peruvianus plants regenerated from callus tissues. The differential patterns of ACP and IDH isozymes and the similar zymograms for PER and EST isozymes presented by callus tissues were used in a comparison of callus tissues cultured for 2 years. The comparative analysis of zymograms within each enzyme system indicated a mean heterogeneity coefficient of 0.33 forC. peruvianus calli cultured for 2 years. Because of the isozyme variations, which developed in culture medium and were transferred to the regenerated plants, the IDH, ACP, PER, and EST enzyme systems can be considered to be good markers for investigating possible genetic variations in plant populations ofC. peruvianus obtainedin vitro from callus culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00555827
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissues and callus cultures ofCereus peruvianus (cactaceae) (1993)
    Springer
    Biochemical genetics 31 (1993), S. 167-172 
    Details
    ISSN: 1573-4927
    Keywords: malate dehydrogenase ; cactaceae ; callus culture ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes were investigated in seeds and in seedlings and calli cultures ofC. peruvianus to determine if the changes in MDH isozyme banding patterns could be used as biochemical markers to identify the origin of regenerated plants from callus tissues. Four cytoplasmic MDH isozymes (sMDH), five mitochondrial MDH isozymes (mMDH), and one glyoxysomal MDH isozyme (gMDH) were detected and showed tissue- and stage-specific expression. A relationship of mMDH and gMDH isozyme patterns with callus tissues subcultured in three hormonal combinations and with the plants regenerated from these callus tissues was demonstrated. Furthermore, temperature and mechanical stress were found to be closely related to mMDH-1 activity in callus culture. Therefore, the different patterns of MDH isozymes in the various tissues ofC. peruvianus can be used as biochemical markers for the study of gene expression during development and as powerful tools in monitoring studies on callus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553853
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Alcohol dehydrogenase (EC 1.1.1.1) isozymes as markers at 2,4-dichlorophenoxyacetic acid × kinetin combinations in callus cultures ofCereus peruvianus (Cactaceae) (1994)
    Springer
    Biochemical genetics 32 (1994), S. 191-200 
    Details
    ISSN: 1573-4927
    Keywords: alcohol dehydrogenase ; isozymes ; callus cultures ; cactaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Alcohol dehydrogenase (ADH; EC 1.1.1.1) isozymes were investigated in tissue ofCereus peruvianus cultured in different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin. Five ADH isozymes were detected in starch gel and showed different patterns in seeds, seedlings, calli cultured at 32 and 22°C, and plants regenerated from calli cultured in three 2,4-D and kinetin combinations. Four phenotypes formed by different combinations of ADH-2, ADH-3, ADH-4, and ADH-5 were detected in calli cultured at 32°C and in plants regenerated from calli. ADH-1 isozyme was detected only in calli subcultured for 1 or 2 weeks at 22°C and was indicated as a marker of stress conditions that affect the growth ofC. peruvianus callus tissues in culture. ADH phenotypes with either a higher or a lower number of isozymes were detected in different proportions in the callus tissues cultured in media containing different 2,4-D and kinetin ratios. ADH isozyme patterns were found to be sensitive markers at the highest kinetin concentration or at high kinetin/2,4-D ratios. The results indicate a high correlation between the ADH isozyme patterns and the capacity for regeneration. Thus, ADH isozymes are indicated as good biochemical markers and as a powerful tool for monitoring studies ofC. peruvianus callus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554622
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Glycerol-3-phosphate dehydrogenase (G-3-PDH; EC 1.1.1.8) variation in Brazilian stingless bees and in wasp species (1991)
    Springer
    Biochemical genetics 29 (1991), S. 255-260 
    Details
    ISSN: 1573-4927
    Keywords: glycerol-3-phosphate dehydrogenase ; social bees ; wasp ; isozymes ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In only 1 bee species(Tetragona clavipes) of 24 sampled in 145 colonies (0.69%) did we detect the presence of more than one allele for glycerol-3-phosphate dehydrogenase (EC 1.1.1.8), an enzyme that is involved in flight. In 34 colonies containing 9 wasp species, 5 colonies of only 2 species(Polybia paulista andP. sericea) showed variation in larval G-3-PDH (14.7%). The small amount of variation observed for theG-3-PDH-1 locus in the bee and wasp species analyzed in the present study agrees with that reported for the G-3-PDH system in other insects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00590105
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Glycerol-3-phosphate dehydrogenase isozyme variation in adult meliponids (Hymenoptera: Apidae) (1991)
    Springer
    Biochemical genetics 29 (1991), S. 593-600 
    Details
    ISSN: 1573-4927
    Keywords: glycerol-3-phosphate dehydrogenase ; bees ; wasps ; isozymes ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Glycerol-3-phosphate dehydrogenase (G-3-PDH) isozymes were investigated in several bee and wasp species to verify if variations detected in G-3-PDH-2 isozymes are closely related to the age and activity of adult workers in the nest or hive of social species. In the solitary, the semisocial, and one social bee species, no phenotypic variations were detected for G-3-PDH-2 isozymes, and this was also the case for all wasp species investigated which were characterized as social. These results allow us to suggest that the variation detected in G-3-PDH-2 isozymes is a phenomenon closely related not only to adult age and activity in the hive, but also to a gradual acquisition of the ability to fly, which is not present in newly emerged worker meliponids in particular.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554132
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Glycerol-3-phosphate dehydrogenase isozyme variation in adult meliponids (Hymenoptera: Apidae) (1991)
    Springer
    Biochemical genetics 29 (1991), S. 593-600 
    Details
    ISSN: 1573-4927
    Keywords: glycerol-3-phosphate dehydrogenase ; bees ; wasps ; isozymes ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Glycerol-3-phosphate dehydrogenase (G-3-PDH) isozymes were investigated in several bee and wasp species to verify if variations detected in G-3-PDH-2 isozymes are closely related to the age and activity of adult workers in the nest or hive of social species. In the solitary, the semisocial, and one social bee species, no phenotypic variations were detected for G-3-PDH-2 isozymes, and this was also the case for all wasp species investigated which were characterized as social. These results allow us to suggest that the variation detected in G-3-PDH-2 isozymes is a phenomenon closely related not only to adult age and activity in the hive, but also to a gradual acquisition of the ability to fly, which is not present in newly emerged worker meliponids in particular.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426873
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissues and callus cultures ofCereus peruvianus (cactaceae) (1993)
    Springer
    Biochemical genetics 31 (1993), S. 167-172 
    Details
    ISSN: 1573-4927
    Keywords: malate dehydrogenase ; cactaceae ; callus culture ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes were investigated in seeds and in seedlings and calli cultures ofC. peruvianus to determine if the changes in MDH isozyme banding patterns could be used as biochemical markers to identify the origin of regenerated plants from callus tissues. Four cytoplasmic MDH isozymes (sMDH), five mitochondrial MDH isozymes (mMDH), and one glyoxysomal MDH isozyme (gMDH) were detected and showed tissue- and stage-specific expression. A relationship of mMDH and gMDH isozyme patterns with callus tissues subcultured in three hormonal combinations and with the plants regenerated from these callus tissues was demonstrated. Furthermore, temperature and mechanical stress were found to be closely related to mMDH-1 activity in callus culture. Therefore, the different patterns of MDH isozymes in the various tissues ofC. peruvianus can be used as biochemical markers for the study of gene expression during development and as powerful tools in monitoring studies on callus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02399923
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Isozyme Variability in Plants Regenerated from Calli of Cereus peruvianus (Cactaceae) (1997)
    Springer
    Biochemical genetics 35 (1997), S. 189-204 
    Details
    ISSN: 1573-4927
    Keywords: somaclonal variation ; tissue culture ; isozymes ; cactus ; genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Morphological and isozyme variation was observed among plants regenerated from callus cultures of Cereus peruvianus. Different morphological types of shoots (68%) were observed in 4-year-old regenerated plants, while no distinct morphological variants were observed in plants grown from germinated seeds. Isozyme patterns of 633 plants regenerated from calli and of 261 plants grown from germinated seeds showed no variation in isocitrate dehydrogenase isozyme, and the differential sorbitol dehydrogenase, alcohol dehydrogenase, malate dehydrogenase, acid phosphatase, and peroxidase isozyme patterns observed in regenerated plants were attributed to nonallelic variation. Allelic variation was detected at three isoesterase loci. The proportion of polymorphic loci for both populations was 13.6% and the deviation from Hardy–Weinberg equilibrium for the Est-1 and Est-7 loci observed in somaclones was attributed to the manner in which the regenerant population was established. The high values for genetic identity among regenerant and seed-grown plant populations are in accordance with the low levels of interpopulation genetic divergence. In somaclones of C. peruvianus, morphological divergence was achieved within a short time but was not associated with any isozyme changes and also was not accompanied by biochemical genetic divergence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1021906226291
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...