ISSN:
1573-3904
Keywords:
chemoselective ligation
;
Fmoc-methodology
;
iso-thiouronium salts
;
Kenner safety catch resin
;
native chemical ligation
;
peptide thioesters
;
peptide isomerisation
;
safety-catch linkers
;
solid-phase synthesis
;
thioester exchange
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract We describe, and compare, two methods which enable theassembly of proteins from peptide thioester fragmentsprepared by Fmoc chemistry mediated solid phase synthesis. The first, which utilizes iso-thiouronium salts,allows formation of thiophenyl esters directly frompartially protected peptides, either in solution or onresin. The second uses ‘sulfonamide’ based safety-catchresins. Data on yields, generality and potential for side-reactions are provided.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1011849912229
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