ISSN:
1573-4986
Keywords:
neuropsin
;
N-glycan structures
;
insect cells
;
2/3-D sugar mapping
;
Fuc, L-fucose
;
HPLC, high performance liquid chromatography
;
Glc unit, glucose unit
;
PA, 2-pyridylamino-
;
ODS, octadecylsilica
;
T. ni, Trichoplusia ni
;
2-D, two dimensional
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract N-glycans of neuropsin (serine protease in the murine hippocampus) expressed in Trichoplusia ni cells were released from the glycopeptides by digestion with glycoamidase A (from sweet almond), and the reducing ends of the oligosaccharides were reductively aminated with 2-aminopyridine. The derivatized N-glycans were separated and structurally identified by a two dimensional high-performance liquid chromatography (HPLC) mapping technique on two kinds of HPLC columns. Fourteen different major N-glycan structures were identified, of which 6 were high-mannose type (9.1%), and the remaining 8 were paucimannosidic type. The presence of insect specific N-glycan structures containing both α1,3- and α1,6- di-fucosylated innermost N-acetylglucosamine residue (23.3%), as below, was also confirmed by 600 MHz 1H-NMR spectroscopy.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007082612019
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