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  • indole-3-acetic acid  (3)
  • Springer  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Transport and metabolism of indole-3-butyric acid in cuttings of Leucadendron discolor (1993)
    Springer
    Plant growth regulation 12 (1993), S. 17-22 
    Details
    ISSN: 1573-5087
    Keywords: indole-3-acetic acid ; indole-3-butyric acid ; IBA-aspartic acid ; IBA-glucose ; protea ; rooting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole-3-butyric acid (IBA) greatly enhanced the rooting of an early-flowering variety of protea, Leucadendron discolor, but had very little effect on a late-flowering variety. IBA transport and metabolism were studied in both varieties after incubating the cuttings in 3H-IBA. More of the radio-label was transported to the leaves of the easy-to-root variety than the difficult-to-root (35–45% and 10%, respectively). IBA was metabolized rapidly by the cuttings of both varieties and after 24 h most of the label was in the new metabolite. However, free IBA (about 10%) was present in the cuttings during the whole period up to the time of root emergence (4 weeks). More free IBA was accumulated in the base of easy-to-root cuttings, while in the difficult-to-root variety most of the IBA was found in the leaves. The metabolite was identified tentatively as an ester conjugate with a glucose. It is possible that IBA-glucose serves as a source for free IBA, and the difference between the varieties is a consequence of the free IBA which is released, transported and accumulated in the site of a root formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00144577
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  • 2
    Electronic Resource
    Electronic Resource
    Indole-3-butyric acid in Arabidopsis thaliana III. In vivo biosynthesis (1994)
    Springer
    Plant growth regulation 14 (1994), S. 7-14 
    Details
    ISSN: 1573-5087
    Keywords: Arabidopsis thaliana ; IBA biosynthesis ; indole-3-acetic acid ; indole-3-butyric acid ; α-naphthylacetic acid ; phenylacetic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole-3-butyric acid (IBA) was identified by HPLC and GC-MS as one of the reaction products after incubation of sterile cultures of Arabidopsis thaliana seedlings with labeled indole-3-acetic acid (IAA). This is the first demonstration of IBA biosynthesis in a dicotyledonous plant. After 1 h of incubation most of the IBA was found in the free form, while after longer periods of incubation most of it was detected in conjugated forms. Formation of IBA conjugates was inhibited by the addition of unlabeled IBA. The biosynthesis of IBA and its conjugates was followed throughout the development of the seedlings and at different pH values. All parts of the plant (isolated roots, leaves, shoots and flowers) were able to convert IAA to IBA to the same extent. IAA was more readily transported than IBA in mature Arabidopsis plants. Feeding of labeled phenylacetic acid (PAA) and α-naphthylacetic acid (NAA) to Arabidopsis seedlings resulted in a new small peak which was hydrolyzed by 7N NaOH, but the formation of compounds with longer side chains (analogous to IBA) could not be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024135
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  • 3
    Electronic Resource
    Electronic Resource
    Indole-3-butyric acid in Arabidopsis thaliana (1993)
    Springer
    Plant growth regulation 13 (1993), S. 179-187 
    Details
    ISSN: 1573-5087
    Keywords: Arabidopsis thaliana ; auxin conjugates ; ethylene ; indole-3-acetic acid ; indole-3-butyric acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole-3-butyric acid (IBA) was identified by HPLC and GC-MS as an endogenous compound in plantlets of the crucifer Arabidopsis thaliana (L.) Heynh. A. thaliana was cultivated under sterile conditions as shaking culture in different liquid media with and without supply of hormones. Free and total IBA and indole-3-acetic acid (IAA) were determined at different stages of development during the culture period as well as in culture media of different initial pH values. The results showed that IAA was present in higher concentrations than IBA, but both hormones seemed to show the same behaviour under the different experimental conditions. Differences were found in the mode of conjugation of the two hormones. While IAA was mostly conjugated via amide bonds, the main IBA conjugates were ester bound. The ethylene concentration derived from the seedlings, when they were grown in flasks of different size, seemed not to influence the auxin content in the same cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024260
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