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  • hydrophobic areas  (2)
  • maytansine  (1)
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  • 1
    Digitale Medien
    Digitale Medien
    Interaction of Phomopsin A with Normal and Subtilisin-Treated Bovine Brain Tubulin (1997)
    Springer
    The protein journal 16 (1997), S. 99-105 
    Details
    ISSN: 1573-4943
    Schlagwort(e): Tubulin ; phomopsin A ; alkylation ; hydrophobic areas ; C-terminal domain
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract Tubulin, the major component of microtubules, has a tendency to lose its ability to assemble or to bind to ligands in a time-dependent process known as “decay.” The decay process also causes tubulin to expose sulfhydryl groups and hydrophobic areas. The antimitotic drug phomopsin A strongly protects the tubulin molecule from decay. Here we have studied the interaction of phomopsin A with αβ tubulin and tubulin which has been treated with subtilisin to remove selectively the C-termini of the α and β chains (αsβs). The binding of phomopsin A to αβ tubulin decreases the sulfhydryl titer by approximately 1.0 mol/mol. Selective removal of the peptides from the C-terminal ends does not affect phomopsin A's interaction with tubulin. Moreover, the αsβs tubulin–phomopsin A complex appears to be more stable than the αβ tubulin–phomopsin A complex as determined by the time-dependent increase in exposure of sulfhydryl groups and hydrophobic areas on tubulin. In fact, phomopsin A inhibits the decay process of αsβs tubulin completely. This observation raises the possibility of determining the conformtion of this configuration of tubulin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1026337900317
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Removal of the projection domain of microtubule-associated protein 2 alters its interaction with tubulin (1994)
    Springer
    The protein journal 13 (1994), S. 381-391 
    Details
    ISSN: 1573-4943
    Schlagwort(e): Vinblastine ; tubulin ; microtubule-associated proteins ; tau ; maytansine
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract Microtubule-associated proteins (MAPs) can promote microtubule assemblyin vitro. One of these MAPs (MAP2) consists of a short promoter domain which binds to the microtubule and promotes assembly and a long projection domain which projects out from the microtubule and may interact wth other cytoskeletal elements. We have previously shown that MAP2 and another MAP, tau, differ in their interactions with tubulin in that tau, but not MAP2, promotes extensive aggregation of tubulin into spiral clusters in the presence of vinblastine and that microtubules formed with MAP2 are more resistant than those formed with tau to the antimitotic drug maytansine [Luduena, R. F.,et al. (1984),J. Biol. Chem. 259, 12890–12898; Fellous, A.,et al. (1985),Cancer Res. 45, 5004–5010]. Here we have used chymotryptic digestion to remove the projection domain of MAP2 and examined the interaction of the digested MAP2 (ctMAP2) with tubulin in the presence of vinblastine and maytansine. We have found that ctMAP2 behaves very much like tau, but not like undigested MAP2, in the presence of vinblastine, in that ctMAP2 causes tubulin to polymerize into large clusters of spirals. In contrast, microtubule assembly in the presence of ctMAP2 is much more resistant to maytansine inhibition than is assembly in the presence of tau or undigested MAP2. Our results suggest that the projection domain of MAP2 may play a role in the interaction of tubulin with MAP2 during microtubule assembly.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01901694
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Tubulin Stability and Decay: Mediation by Two Distinct Classes of IKP104-Binding Sites (1998)
    Springer
    The protein journal 17 (1998), S. 303-309 
    Details
    ISSN: 1573-4943
    Schlagwort(e): Tubulin ; IKP104 ; alkylation ; hydrophobic areas ; C-terminal domain
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract IKP104, a novel antimitotic drug, has two classes of binding sites on bovine brain tubulin with different affinities. IKP104, by itself, enhances the decay of tubulin, but in the presence of colchicine or podophyllotoxin, it stabilizes tubulin instead of opening up the hydrophobic areas [Luduena et al. (1995), Biochemistry 34, 15751–15759], Here, we have dissected these two apparently contradictory effects of IKP104 by cleaving the C-terminal ends of both α and β subunits of tubulin with subtilisin. We have found that the selective removal of the C-terminal ends from both the α and β subunits of αβ tubulin lowers the sulfhydryl titer by approximately 1.5 mol/mol of dimer. Interestingly, IKP104 does not increase either the sulfhydryl liter or the exposure of hydrophobic areas of this subtilisin-treated tubulin (αsβs). Moreover, IKP104 lowers the sulfhydryl titer of αsβs tubulin approximately by 1 mol/mol and appears to inhibit completely the time-dependent decay of αsβs tubulin. The cleavage at the C-terminal ends of both α and β modulates the effect of IKP104 on the β subunit, but not on the α subunit. Fluorometric binding data analysis suggests that IKP104 binds to the αsβs tubulin only at the high-affinity site; the low-affinity site(s) disappear almost completely. The sulfhydryl titer data for α and β and the fluoromelric data therefore suggest that the interaction of IKP104 at the high-affinity site on tubulin is not regulated by the C-terminal domains of α and β and the effect of the high-affinity site is restricted largely to the α subunit, while the low-affinity-site binding is modulated by the C-terminal domain of β. It also appears that the stabilization and the acceleration of the decay of tubulin are mediated by distinct interactions of IKP104 with its high- and low-affinity sites on tubulin, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1022579930546
    Standort Signatur Erwartet Verfügbarkeit
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