ISSN:
1573-5001
Schlagwort(e):
CI2
;
chemical exchange
;
constant-time
;
hydration
;
lysozyme
;
NOE
;
quadrature detection
;
ROE
;
water
;
water flip-back
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract A pulse sequence is proposed to select water magnetization with enhanced specificity through a synergetic combination of several filtering principles. This approach relies on a constant-time evolution period implemented without quadrature detection, which results in a √2 increase in signal-to-noise ratios as compared to traditional non-selective methods for water filtration. In addition, the quadrature-free constant-time block facilitates the implementation of the water flip-back strategy, which leads to further gains in sensitivity. The proposed experiment was applied to unlabeled HEW lysozyme and to 15 N-labeled chymotrypsin inhibitor 2 which was partially or non 13C-enriched. Water molecules belonging to a spine of hydration between two pseudo β-sheet strands were identified, solving previously reported discrepancies between the X-ray and the refined NMR structure of CI2. The proposed experiment is particularly suitable for hydration studies of mixtures of labeled and unlabeled components, such as ligand–macromolecule complexes.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1008316612907
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