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  • human red cells (or erythrocytes)  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    A method for estimating free Ca within human red blood cells, with an application to the study of their Ca-dependent K permeability (1982)
    Springer
    The journal of membrane biology 66 (1982), S. 235-247 
    Details
    ISSN: 1432-1424
    Keywords: human red cells (or erythrocytes) ; intracellular free Ca ; digitonin ; null-point ; Ca-dependent K permeability (or K permeability)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Murphy, Coll, Rich and Williamson (J. Biol. Chem. 255:6600–6608, 1980) described a null-point method for estimating intracellular free Ca in liver cells. They used digitonin to lyse the cells in solutions of varying Ca concentration. This method has been adapted for use with human red cells. The values found are about 0.4 μm Ca in fresh cells, and from 0.4 to 0.7 μm Ca in blood-bank cells, at pH 7.2 and 37°C. These are likely to be overestimates, and the errors and limitations of the method are discussed. Red cells may be loaded with Ca by metabolic depletion in Ca-containing solutions. Such cells have an elevated K permeability, and the relationships between free Ca, total Ca and K permeability were investigated, using86Rb as a tracer for K.86Rb flux studies show that the affinity of the K channel for Ca is the same in cells as in resealed ghosts where intracellular Ca can be controlled with Ca buffers, but the rate of tracer equilibration is 3–6 times faster in ghosts than in cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868498
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of lead ions on cation permeability in human red cell ghosts (1985)
    Springer
    The journal of membrane biology 84 (1985), S. 61-71 
    Details
    ISSN: 1432-1424
    Keywords: human red cells (or erythrocytes) ; Ca-dependent K permeability (or K permeability) ; Pb ; Pb ion buffers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Intracellular Pb2+ ions can replace Ca2+ ions in stimulating the Ca-dependent K permeability of human red blood cells. In metabolically depleted resealed ghosts, the threshold for stimulation of86Rb efflux by internal Pb2+ is around 5×10−10 m, and stimulation is half-maximal at about 2×10−9 m, and maximal at 10−8 m Pb2+. There is no effect on22Na efflux in this concentration range.86Rb efflux is antagonized by internal Mg2+ ions, and by the channel-blocking drugs quinidine and diS-C2(5), as observed for the Ca-dependent K permeability in red cells. In ghosts containing EDTA, which prevents any internal effects of Pb2+ ions, external Pb2+ increases both22Na and86Rb permeability when its concentration exceeds 6×10−7 m. This effect is seemingly unrelated to the Ca-dependent K permeability. This work makes extensive use of Pb2+ ion buffers, and gives information about their preparation and properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871648
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