ISSN:
1420-9071
Keywords:
Thyroid hormone
;
human corpus luteum
;
triiodothyroinine binding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A high affinity, low capacity 3,5,3′-triiodo-L-thyroinine (T3) specific binding was detected in the nuclei preparation from human corpus luteal nuclei by using an in vitro thyroid hormone binding assay. The bound hormone was effixciently separated from free hormone by the use of 40% polyetrhylene glycol. Under standardized assay conditions of pH 8.6 at 37°C and a 2-h incubation time, the binding in the corpus luteal nuclei was saturable with Kd 4.94×10−10 M with low maximum binding capacity (1.70 p. mol/mg DNA). Competitive binding studies with hormone analogues indicated that T3 binding to corups luteal nuclei is hormone-specific. Findings indicated a driect effect of thyroid hormone on the human corpus leteum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01939903
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