ISSN:
1573-0603
Keywords:
growth
;
differentiation
;
mammary epithelium
;
collagen gel
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mammary glands are enzymatically dissociated and the resulting tissue digest enriched for epithelial cells by isopycnic banding on a density gradient of Percoll. The cells are embedded within a rat tail collagen gel matrix and fed with the appropriate medium. Growth and differentiation are superior in such a system when compared to culture on plastic, using identical media.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01834632
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