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  • 1
    ISSN: 1572-9788
    Keywords: AFLP ; essential derivation ; genetic conformity ; Lolium perenne ; variety
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The concept of genetic conformity lies at the basis of the definition of essential derivation, or the process of using a protected variety (or `initial variety', IV) as the base to develop another similar variety (the essentially derived variety, EDV). Methods based on morphology, biochemistry or on molecular markers can be used to estimate genetic conformity. In this study, the capability of AFLP®1 markers to provide a reliable and meaningful estimate of genetic conformity of different varieties was investigated in diploid perennial ryegrass (Lolium perenne spp.), an allogamous species whose varieties are genetically heterogeneous. Twelve accessions with known breeding lineage, comprising five closely related groups, were included in the study. For the set of test accessions analysed, the AFLP protocol accurately reproduced the same relationships as were evident from examining their morphology and both these results were consistent with the relationships known to exist within the different test groups. Principal components analysis as well as cluster analysis associated unambiguously the IV and the EDV accessions. It was concluded that the methodology developed in this study could be used as a model from which to create a protocol for evaluating putative cases of essential derivation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-9788
    Keywords: essential derivation ; variety ; Lolium perenne ; genetic conformity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this study the morphological and protein diversity of twelve diploid perennial ryegrass accessions (Lolium perenne L.) was examined. These accessions comprised five closely related groups, each containing an `initial variety' (IV) and one or more declared `essentially derived varieties' (EDV), with differing degrees of relatedness. `Essential derivation' is a legal concept relating to intellectual property in plant varieties and is additional to Plant Breeders Rights (PBR). An EDV is defined as clearly distinct from, but conforming in its expression of the essential characteristics of an IV, from which it is found to have been predominantly derived. Where an essential derivation has been confirmed, the breeder of the IV may be entitled to some royalty sharing of the EDV. Clearly, therefore, in any successful EDV claim, evidence of a high degree of conformity in either the phenotype or genotype would be required. Examination of plant morphology indicated that all the EDVs were morphologically distinct from their corresponding IV in one or more morphological characteristic. using a principal co-ordinates analysis to give an overall measure of morphological difference, all twelve accessions were correctly clustered into their related groups and the magnitude of the differences within groups reflected their known breeding histories. Examining protein diversity by methods that targeted single- and multiple-locus genes also clustered the accessions into their correct groups, in most cases. However, only by examining diversity in seed storage proteins were within-group relationships accurately represented. The methods used provided no consistent representation of between-group relationships. It was concluded that the morphological method provided a creditable measure of genetic conformity, but to avoid incurring excessive time, work and cost, results from existing national PBR trials would need to be openly available. Within the limits of the genetic material examined, seed storage protein diversity appeared to provide a suitable combination of accuracy and efficiency on which to base a routine test. However, given more complex breeding relationships than those in this study, methods such as AFLP1 markers that sample more genetic diversity, may be necessary to maintain this level of accuracy.
    Type of Medium: Electronic Resource
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