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  • Chondrichthyes  (3)
  • Triticum  (2)
  • etintidine  (2)
  • glycogen phosphorylase  (2)
  • Springer  (9)
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  • Springer  (9)
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 117 (1992), S. 63-70 
    ISSN: 1573-4919
    Schlagwort(e): glycogen phosphorylase ; alloxan-diabetes ; cardiomyocytes ; G-protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The basis for the hypersensitive response of glycogen phosphorylase to epinephrine stimulation was investigated in adult rat cardiomyocytes isolated from normal and alloxan-diabetic animals. To assess potential G-protein involvement in the response, normal and diabetic derived myocytes were incubated with either cholera or pertussis toxin prior to hormonal stimulation. Pretreatment of cardiomyocytes with cholera toxin resulted in a potentiated response to epinephrine stimulation whereas pertussis toxin did not affect the activation of this signaling pathway. To determine if the enhanced response of phosphorylase activation resulted from an alteration in adenylate cyclase activation, the cells were challenged with forskolin. After 3 hr in primary culture, diabetic cardiomyocytes exhibited a hypersensitive response to forskolin stimulation relative to normal cells. However, after 24 hr in culture, both normal and diabetic myocytes responded identically to forskolin challenge. The present data suggest that a cholera toxin sensitive G-protein mediates the hypersensitive response of glycogen phosphorylase to catecholamine stimulation in diabetic cardiomyocytes and this response which is present in alloxan-diabetic cells and is induced in vitro in normal cardiomyocytes is primarily due to a defect at a post-receptor site.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 145 (1995), S. 131-139 
    ISSN: 1573-4919
    Schlagwort(e): glycogen phosphorylase ; alloxan-diabetes ; cardiomyocytes ; cGMP ; phosphodiesterase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The focus of this study was to identify the molecular basis for the hypersensitive response of glycogen phosphorylase activation to epinephrine stimulation in alloxan diabetic-derived cardiomyocytes. Cyclic AMP levels were found not to be significantly different between normal and diabetic-derived cells while cGMP concentrations were found consistently to be significantly lower in diabetic-derived cells than in normal cells. Treatment with cyclic GMP analogues did not affect phosphorylase activation by epinephrine in normal cardiomyocytes whereas, IBMX, a nonselective phosphodiesterase inhibitor, had a significant effect on basal and agonist-stimulated phosphorylase activity in both normal and diabetic-derived cardiomyocytes. Differences in the time course for the rate of decay of phosphorylasea from agonist-stimulated to basal levels were observed between normal and diabetic cells. After 3 h in primary culture, phosphorylasea activity returned to basal levels more quickly in normal than in diabetic-derived cells while after 24 h in culture, the time for phosphorylasea decay was not significantly different between normal and diabetic myocytes and was longer than the 3 h response. After 3 h in primary culture, no significant difference in phosphorylase kinase activity was observed between normal and diabetic-derived cells exposed to epinephrine whereas, after 24 h in culture, phosphorylase kinase activity was significantly decreased in diabetic cells under basal and agonist-stimulated conditions. These data collectively suggest that the hypersensitive response of glycogen phosphorylase to epinephrine stimulation in diabetic-derived cardiomyocytes is not due to a defect present at the level of phosphorylase kinase but may, in part, result from an alteration in cardiac phosphodiesterase activity resulting from diminished intracellular cyclic GMP concentrations.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Planta 192 (1993), S. 104-109 
    ISSN: 1432-2048
    Schlagwort(e): Aluminum toxicity ; Calcium displacement ; Electrical potential ; Root ; Triticum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Several mineral rhizotoxicities, including those induced by Al3+, H+, and Na+, can be relieved by elevated Ca2+ in the rooting medium. This leads to the hypothesis that the toxic cations displace Ca2+ from transport channels or surface ligands that must be occupied by Ca2+ in order for root elongation to occur. In this study with wheat (Triticum aestivum L.) seedlings, we have determined, in the case of Al3+, that (i) Ca2+, Mg2+, and Sr2+ are equally ameliorative, (ii) that root elongation does not increase as Ca2+ replaces Mg2+ or Sr2+ in the rooting media, and (iii) that rhizotoxicity is a function solely of Al3+ activity at the root-cell membrane surface as computed by a Gouy-Chapman-Stern model. The rhizotoxicity was indifferent to the computed membrane-surface Ca2+ activity. The rhizotoxicity induced by high levels of tris(ethylenediamine)cobaltic ion (TEC3+), in contrast to Al3+, was specifically relieved by Ca2+ at the membrane surface. The rhizotoxicity induced by H+ exhibited a weak specific response to Ca2+ at the membrane surface. We conclude that the Ca2+-displacement hypothesis fails in the case of Al3+ rhizotoxicity and that amelioration by cations (including monovalent cations) occurs because of decreased membrane-surface negativity and the consequent decrease in the membrane-surface activity of Al3+. However, TEC3+, but not Al3+, may be toxic because it inhibits Ca2+ uptake. The nature of the specific H+-Ca2+ interaction is uncertain.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Planta 192 (1993), S. 98-103 
    ISSN: 1432-2048
    Schlagwort(e): Aluminum toxicity ; Calcium uptake ; Growth inhibition ; Root ; Triticum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The cation Al3+ is toxic to plants at micromolar concentrations and can severely inhibit root growth in solution experiments. Trivalent aluminum hydrolyzes in solution, and, apart from the Al3+ ion, which dominates speciation below pH 5.0, various mononuclear and polynuclear hydroxy-Al species can also occur (Kinraide 1991). Accumulating evidence suggests that Al3+ is the rhizotoxic species under the experimental conditions used in the present study (Kinraide 1991; Kinraide et al. 1992). The inhibition of Ca2+ uptake in roots by Al3+ has been proposed as a possible mechanism for Al3+ toxicity, and in this study the hypothesis was tested directly. Root growth and Ca2+ uptake were measured in 5-d-old seedlings of wheat (Triticum aestivum L. Thell) during exposure to Al3+ in a low-Ca2+ basal medium, and to Al3+ in the presence of added cations. Uptake of Ca2+ in whole roots and translocation to the shoot were measured using 45Ca2+, and localized measurements of net Ca2+ flux were also made at the root apex using the technique of microelectrode ion-flux estimation. Treatment with 2.64 μM AlCl3 in 226 μM CaCl2, at pH 4.5, severely inhibited root growth without affecting Ca2+ uptake. Addition of 30 mM Na2+, 3 mM Mg2+ or 50 μM tris(ethylenediamine)cobalt(III) to this Al3+ treatment restored root growth but significantly reduced Ca2+ uptake measured over the entire root system and at the root apex. The Al3+ and Ca2+ concentrations were adjusted so that the activities of the Al3+ and Ca2+ ions were constant in all solutions (1.5 μM and 200 μM, respectively). Root growth can be severely inhibited by Al3+ concentrations that do not affect Ca2+ uptake, while the addition of ameliorating cations depresses Ca2+ uptake. These results argue against the hypothesis that Al3+ inhibits root growth by reducing Ca2+ uptake.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Journal of pharmacokinetics and pharmacodynamics 15 (1987), S. 557-568 
    ISSN: 1573-8744
    Schlagwort(e): etintidine ; propranolol ; 4-hydroxypropranolol ; interaction ; pharmacokinetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract Etintidine HCl is a potent H2 -blocker. The effect of clinical doses of etintidine on the disposition of a single oral dose of propranolol was investigated in 12 normal subjects. This was a double-blind, two-way crossover study. Each subject received etintidine (400 mg) or placebo twice a day with meals for 4 days on two occasions (separated by 4 days). On each occasion, the subjects were fasted overnight on Day 3 and were given an oral dose of Inderal® (40 mg propranolol hydrochloride) 30 min following the administration of the morning dose of etintidine or placebo on Day 4. Blood samples were collected prior to and up to 24 hr following the administration of propranolol. The plasma samples were analyzed for propranolol and 4-hydroxypropranolol by HPLC. Comparison of the pharmacokinetic parameters of propranolol between etintidine and the placebo groups indicates that etintidine significantly increased the AUC0−∞,values (573.5 vs. 146.4 ng·hr/ml, p=0.0001)and prolonged the elimination half-life (4.61 vs. 2.33 hr) of propranolol. Statistical evaluation of the pharmacokinetic parameters of 4-hydroxypropanolol indicates that etintidine also increased the AUC0−24 values (43.8 vs. 16.4 ng·hr/ml, p=0.0028) and prolonged the elimination half-life (4.87 vs. 1.97 hr) of 4-hydroxypropranolol. The data suggest that etintidine, like cimetidine, impaired the elimination of propranolol. Etintidine also protracted the elimination of 4-hydroxypropranolol, an active metabolite of propranolol.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    ISSN: 1573-904X
    Schlagwort(e): etintidine ; high-performance liquid chromatography (HPLC) ; solid extraction ; determination of etintidine in plasma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract This paper describes a new, rapid solid extraction method for the determination of etintidine in plasma. The method employs a semiautomatic sample preparation system. Plasma samples and the internal standard (cimetidine) were applied onto octyl-bonded silica extraction columns. The extraction columns were then subjected to Tris buffer and water wash and were subsequently loaded onto an automatic sample injection system. The contents of the extraction columns were eluted on-line with a mobile phase of acetonitrile:methanol:0.1% ammonium hydroxide (85:10:5, by volume) onto a silica analytical column and detected by UV absorption at 229 nm. The chromatographic condition separates etintidine from some of its metabolites and other endogenous components in plasma. The detection limit for etintidine was 0.02–0.05 µg/ml when 0.2 ml of plasma was used. This method has been used for the determination of plasma etintidine levels in humans and mice after oral administration of etintidine and was found to be suitable for pharmacokinetic/bioavailability studies of etintidine in humans and animals. The method can also be used for the quantitative determination of cimetidine and certain metabolites of etintidine.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 1573-5133
    Schlagwort(e): Batoidea ; Chondrichthyes ; Elasmobranchii ; Amazon ; Magdalena ; Maracaibo ; Orinoco ; Paraná-Paraguay
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Synopsis The number of venomous caudal spines and their length and position relative to one another were determined in seven species of South American freshwater rays (Potamotrygonidae) and eight marine or euryhaline species of four families from the Caribbean Coast of South and Central America. Most species have two visible spines at certain stages in the shedding-replacement cycle and only one visible spine at other stages (following shedding). If we include the embryological beginnings of the spines before they erupt and become visible, the spine counts of most rays are actually 2 rather than 1 or 2. Since most species apparently follow this pattern, spine counts are of little use in distinguishing between species except in the relatively few that may have only one, or no spines. Eight captive Potamotrygon specimens maintained in simulated tropical temperature conditions over 12 months showed periodic shedding and replacement of spines. The molts were biannual for a given ray but annual for a given spine. They alternated between two spine loci and their cycles were approximately six months out of phase with each other. Recent studies on Dasyatis sabina by others report only one molt per year, with replacement spines forming always posterior to the primary spine rather than alternating between posterior and anterior. Supernumerary spines (counts of more than two, up to five) are also discussed, as are counts of one and zero.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Environmental biology of fishes 7 (1982), S. 207-228 
    ISSN: 1573-5133
    Schlagwort(e): Batoids ; Chondrichthyes ; Costa Rica ; Elasmobranchs ; Euryhalinity ; Freshwater adaptation ; Growth rate ; Isolation of population ; Nicaragua
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Synopsis Of a total of 377 Pristis perotteti tagged in the Lake Nicaragua-Río San Juan System, 214 (56.8% were recovered. Eighty were recovered at the original tagging site; four moved downstream the full length of the river; and 127 tagged at the source of the river were recovered in all parts of the lake. Only one was recovered in a different river system, 58 km down the coast from the main mouth of the Río San Juan. A life span of 30 years is suggested, with rapid growth (30–40 cm per year) in the first three years, slowing to about 4 or 5 cm per year in the later years of life. Maximum sizes collected were 384 cm for males, 429 cm for females, smaller than maximum lengths reported elsewhere. The lake sawfish are not physically landlocked, but individuals remain in fresh water for very long periods; parturition takes place in fresh water; all sizes are found in the lake; and it appears that this stock finds all of its ecological needs met in the lake. Individuals may spend all of their lives in fresh water, although, as a species, P. perotteti has not completely abandoned the sea, since some are known to occur in salt water. The Lake Nicaragua-Río San Juan sawfish are a discrete stock, with only limited gene flow with neighboring stocks. P. perotteti is farther along in its adaptation to fresh water, in being able both to osmoregulate and reproduce there, than other known euryhaline elasmobranchs, except for the African stingray, Dasyatis garouaensis, of the Niger-Benue System, and the completely adapted South American freshwater rays (family Potamotrygonidae).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Environmental biology of fishes 9 (1983), S. 3-24 
    ISSN: 1573-5133
    Schlagwort(e): Amazonia ; Batoidea ; Brazil ; Captive breeding ; Chondrichthyes ; Colombia ; Elasmobranchii ; Freshwater adaptation ; Growth rate ; Potamotrygonidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Synopsis Observations of reproductive features and body measurements were made on wild-caught, freshwater stingrays, Potamotrygon circularis and P. motoro, from the Amazon drainage of western Brazil and southern Colombia. Further observations were made in Detroit's Belle Isle Aquarium on a captive pair of P. motoro and their descendants, which constitute the first known captive breeding colony of potamotrygonids. The gross structure and function of female and male reproductive systems are described. There is no obvious difference between those of the two species. They are aplacentally viviparous, the young being nourished in advanced stages by uterine milk secreted by trophonemata. Size at onset and completion of sexual maturation, breeding season and behavior, gestation period, litter size and sex ratios are discussed. Up to 21 proportional measurements were made on several fetal and postnatal stages of both species. Several proportional changes occur in very early fetal life, but most body proportions undergo only minor changes from advanced fetal through adult stages. A growth curve is proposed for P. motoro based on observations of the captive colony.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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