ISSN:
1573-5044
Keywords:
embryogenic calluses
;
immature embryos
;
maize embryogenesis
;
pollen-embryos
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A comparative study of polyamine (putrescine, spermidine and spermine) levels was conducted with maize calli originating from a) immature embryos and b) pollen embryos capable of plant regeneration. The differences observed in the studied parameters of the two kinds of calluses are related to their cellular origin and to their regeneration capacity. Moreover, only the calluses proceeding from immature embryos differentiated into preembryogenic structures, which eventually developed into plants. Although total polyamine levels in pollenderived calluses were significantly higher than those from immature embryos, spermidine and spermine were the predominant polyamines in both culture types. Furthermore, polyamine fractions of these calluses also showed differences. All these phenomena may be related with the differences observed in the callus embryogenic response. These findings may be useful in understanding the implication of polyaminesin embryogenetic processes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00037667
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