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  • electroantennogram  (7)
  • Springer  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 55 (1990), S. 205-212 
    ISSN: 1570-7458
    Keywords: Olfactory sensitivity ; grasshoppers ; electroantennogram ; Larrea ; hostplant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les réponses antennaires ont été examinées par électroantennogramme aux odeurs de plantes hôtes et non hôtes d'orthoptères Gomphocerinae de comportements alimentaires différents: Bootettix argentatus, monophage sur une dicotylédone, Larrea; Ligurotettix coquilletti, oligophage de dicotylédones; Cibolacris parviceps, polyphage; Chorthippus curtipennis, graminivore. Les types de réponse des quatre espèces étaient très voisins, mais les réponses aux odeurs de Larrea de B. argentatus et L. coquilletti, spécialistes de cette plante, étaient relativement plus fortes. Ces résultats suggèrent une similarité globale considérable dans la composition des populations de récepteurs olfactifs de ces quatre espèces d'orthoptères mais aussi une certaine spécificité pour les odeurs de la plante hôte au niveau antennaire.
    Notes: Abstract Antennal responses to host and non-host odours were recorded, using the electroantennogram technique, from four gomphocerine grasshoppers with different feeding habits: Bootettix argentatus (monophagous on Larrea, a dicotyledonous plant), Ligurotettix coquilletti (oligophagous on dicotyledonous plants), Cibolacris parviceps (polyphagous) and Chorthippus curtipennis (graminivorous). The patterns of responses across the plant odours were similar for all four grasshopper species, but there was a relatively greater response to the odour of Larrea in B. argentatus and L. coquilletti which specialise on this plant. This suggests that there is considerable overall similarity in the make up of the olfactory receptor populations of these four grasshoppers species, but that some specificity for hostplant odour does exist at the antennal level.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 19 (1993), S. 2193-2202 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; Dendroctonus valens ; EAG ; electroantennogram ; enantiomer ; kairomone ; host attraction ; bark beetle ; α-pinene ; β-pinene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The antennal response ofDendroctonus valens to host monoterpenes from the resin of ponderosa pine was studied using the electroantennogram (EAG) technique. Male and female beetles were given a single dose of each of 11 different monoterpenes. Response amplitude to the different compounds did not vary between sexes and was generally well correlated with results from field attraction studies. Response to (S)-(−)-β-pinene was greatest. The relative amplitude of the responses to the (R)-(+) and (S)-(−) enantiomers of a-pinene, however, were reversed from their relative attractiveness in the field. A dose-response study was conducted for the (R)-(+) and (S) -(−) enantiomers of a-pinene, plus a reciprocal differential saturation test with successive doses of first one enantiomer ofα-pinene and then the other. Comparison of EAG traces suggests different receptors for the two stereoisomers ofα-pinene. Differential saturation curves suggest that while one set of receptors may respond to both enantiomers, some receptors respond only to the (S)-(−) enantiomer.
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  • 3
    ISSN: 1573-1561
    Keywords: Aggregation pheromone ; Oryzaephilus surinamensis ; sawtoothed grain beetle ; macrolide lactones ; electroantennogram ; GC-EAG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Over 200 beetle- and food-produced volatiles were collected from cultures of the saw-toothed grain beetleOryzaephilus surinamensis (L.) on oats. It proved possible to develop the electroantennogram recording technique for these beetles, despite their small size, allowing volatiles causing antennal responses to be identified by coupled GC-EAG and subsequent GCMS techniques. Three beetle-produced macrolide lactones were identified as (Z,Z)-3,6-dodecadien-11-olide, (Z,Z)-3,6-dodecadienolide, and (Z,Z)-5,8-tetradecadien-13-olide in an average ratio of 4.4∶1∶2. These have been reported as components of the aggregation pheromone from a different population of this species, although the ratio of the components produced was different. Three food volatiles with EAG activity were also identified: 1-octen-3-ol, 3-octanone, and nonanal. A mixture of the six identified volatiles produced similar levels of attraction, in a behavioral assay, to the entire mixture of collected volatiles.
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  • 4
    ISSN: 1573-1561
    Keywords: Cryptolestes ferrugineus ; C. pusillus ; Coleoptera ; Cucujidae ; aggregation pheromone ; macrocyclic lactone ; electroantennogram ; behavioural bioassay ; interspecific response ; thermal desorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Antennal and behavioral responses of the rust-red grain beetle,Cryptolestes ferrugineus, and the flat grain beetle,C. pusillus, to synthetic samples of the macrocyclic lactones reported to comprise their aggregation pheromones were investigated. Electroantennogram (EAG) recordings were obtained successfully from both species for the first time. Females of both species showed larger EAGs than males. The EAGs ofC. ferrugineus showed a high degree of specificity for conspecific aggregation pheromone components;C. pusillus showed much less specificity. Behavioral tests were conducted using two-choice pitfall bioassays. Separation of the results into the two effects of activity stimulation and direction finding showed that both effects contributed to the overall response, although sometimes to different extents. The strain ofC. pusillus studied responded equally well to both components of its pheromone, whereas it had been reported previously that only one was active, the other acting as a Synergist and eliciting no response when tested alone. With both species, behavioral response was elicited with a single lactone, suggesting that it might not be necessary to use both components for field use. Particularly surprising was thatC. pusillus showed a greater response to the pheromone components ofC. ferrugineus than to its own. Aeration of the two species and thermal desorption of the collected volatiles confirmed production of the expected lactones, and aeration of authentic lactones showed that the response was not due to the C.ferrugineus pheromone components being markedly more volatile. This response, which seems to be an actual preference, is the first to be discovered among the cucujid beetles and encourages optimism that a practical lure for various species may not need to be as complex as originally feared.
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  • 5
    ISSN: 1573-1561
    Keywords: Sitophilus granarius ; grain weevil ; Coleoptera ; aggregation pheromone ; electroantennogram ; coupled GC-EAG ; behavioral bioassay ; circular dichroism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Coupled GC-EAG techniques have been applied to the study of volatiles from the grain weevil,Sitophilus granarius. for the first time. The size of EAG response was independent of the sex of the responding insect but was consistently larger to extracts of males than those of females. This difference was reflected in a behavioral preference for the male extracts by mated adults of both sexes tested together and virgin adults of both sexes tested separately. The GC-EAG results provide evidence for two materials that are released specifically by the males. Using circular dichroism. one has been found to be identical stereochemically with the (2S,3R)-sitophilate reported by others as the aggregation pheromone in a different strain. This enhances the prospects for the development of a single pheromone lure that would be generally applicable whatever the origin of the strain. The small amount of sitophilate found in the males suggests that it is not stored in large amounts. The other material, present in such a small amount that it has yet to be fully characterized, elicits a higher antennal activity than sitophilate and may have a significant role to play in enhancing the trap catch of this economically important pest.
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  • 6
    ISSN: 1573-1561
    Keywords: Common furniture beetle ; Anobium punctatum ; drugstore beetle ; Stegobium paniceum ; Coleoptera ; Anobiidae ; sex pheromone ; stegobinone ; 2,3,dihydro-2,3,5-trimethyl-6-(1-methyl-2-oxobutyl)-4H-pyran-4-one ; behavior ; electroantennogram ; species specificity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Observations and reports on the common furniture beetleAnobium punctatum suggested that, on emergence, females use a sex pheromone to attract males. GLC analysis of ovipositor extracts showed the presence of a single component, which was found to be active by EAG and coupled GLC-EAG techniques, and to attract males in both walking and flying assays. The pheromone was identified by GC-MS as 2,3-dihydro-2,3,5-trimethyl-6-(1-methyl-2-oxobutyl)-4H-pyran-4-one (stegobinone), which is the sex pheromone of another anobiid, the drugstore beetle,Stegobium paniceum. MaleA. punctatum responded equally to ovipositor extracts of either species, at both the sensory (EAG) and behavioral levels, which poses the question as to how species specificity in mate attraction is achieved.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 15 (1989), S. 1015-1031 
    ISSN: 1573-1561
    Keywords: Saw-toothed grain beetle ; Oryzaephilus surinamensis ; Coleoptera ; Silvanidae ; aggregation pheromone ; electroantennogram ; behavioral bioassay ; blend ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The antennal and behavioral responses of the saw-toothed grain beetle,Oryzaephilus surinamensis, to the three components of its male-produced aggregation pheromone were investigated. EAG recordings showed no differences between the responses of the two sexes to the synthetic pheromone components. In contrast, laboratory behavioral assays demonstrated marked differences between the sexes. More females than males were consistently attracted to mixtures of the synthetic components, and this bias appeared to be caused by one component in the blend. Altering the blend ratio resulted in changes in the ratio of the sexes attracted. Thus, if, as suggested by preliminary work, males vary the blend produced, this should alter the relative response of the sexes to the aggregation pheromone.
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