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  • 1
    Electronic Resource
    Electronic Resource
    Restriction site variation, gene duplication, and the activity ofsn-glycerol-3-phosphate dehydrogenase inDrosophila melanogaster (1992)
    Springer
    Biochemical genetics 30 (1992), S. 169-188 
    Details
    ISSN: 1573-4927
    Keywords: Drosophila ; glycerol-3-phosphate dehydrogenase ; restriction map ; duplication ; enzyme activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Restriction site variation in a 25-kb region including thesn-glycerol-3-phosphate dehydrogenase (Gpdh) locus has been assessed in 29 single femaleD. melanogaster lines from the Cardwell (Australia, QLD) population. TheGpdh locus was duplicated in about one-third of the lines, although the duplication was incomplete and lacked exons 1 and 2. There was no restriction site variation in the duplicated region. Three insertions were found in the gene region but only one affected GPDH activity. The lines with the duplication had higher levels of GPDH activity and protein amount than did nonduplicated lines. This effect was also observed in lines extracted from two other Australian populations. The duplication is shown to have a similar structure in each population investigated and is also present in populations from China and Africa. It is suggested that the effect of the duplication on GPDH activity, which might be due to structural factors affecting transcription at theGpdh locus, could account for the worldwide distribution of the duplication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553643
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Restriction site variation, gene duplication, and the activity ofsn-glycerol-3-phosphate dehydrogenase inDrosophila melanogaster (1992)
    Springer
    Biochemical genetics 30 (1992), S. 169-188 
    Details
    ISSN: 1573-4927
    Keywords: Drosophila ; glycerol-3-phosphate dehydrogenase ; restriction map ; duplication ; enzyme activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Restriction site variation in a 25-kb region including thesn-glycerol-3-phosphate dehydrogenase (Gpdh) locus has been assessed in 29 single femaleD. melanogaster lines from the Cardwell (Australia, QLD) population. TheGpdh locus was duplicated in about one-third of the lines, although the duplication was incomplete and lacked exons 1 and 2. There was no restriction site variation in the duplicated region. Three insertions were found in the gene region but only one affected GPDH activity. The lines with the duplication had higher levels of GPDH activity and protein amount than did nonduplicated lines. This effect was also observed in lines extracted from two other Australian populations. The duplication is shown to have a similar structure in each population investigated and is also present in populations from China and Africa. It is suggested that the effect of the duplication on GPDH activity, which might be due to structural factors affecting transcription at theGpdh locus, could account for the worldwide distribution of the duplication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02399707
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A KP Element Inserted Between the Two Promoters of the Alcohol Dehydrogenase Gene of Drosophila melanogaster Differentially Affects Expression in Larvae and Adults (1998)
    Springer
    Biochemical genetics 36 (1998), S. 363-379 
    Details
    ISSN: 1573-4927
    Keywords: DROSOPHILA MELANOGASTER ; KP ELEMENT ; TRANSCRIPTION
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An allele of the Drosophila melanogaster alcoholdehydrogenase (Adh) gene has a 1.15-kb KP elementinserted, in the same orientation as Adh transcription,five nucleotides upstream of the distal transcription start site. The target site-GTCCAAGT — inAdh between nucleotides –13 and –6 isduplicated at both ends of the insertion. Adult flieswith either one or two copies of the allele have lessthan 12% of the alcohol dehydrogenase (ADH) activity of thecontrols. Activity levels are also reduced in larvae,but by a much smaller amount. Quantitative Northernanalyses showed that the low activity level in adults was caused by reduced transcript levels fromthe distal promoter. 5′ RACE experiments indicatedthat adult transcripts were mostly initiated downstreamof the distal promoter but some skipped the first adult exon. In late third-instar larvae thetranscripts present were from the proximal promoter.After the KP element was deleted by an appropriatebreeding program, the distal transcript increased inadults to the control level, but ADH activityincreased to only 50% of the control. No nucleotidechanges were found in the gene that could explain thisdifference.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018749513875
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    Paper (German National Licenses)
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