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  • dithiothreitol  (1)
  • organic acids transport  (1)
  • Wiley-Blackwell  (2)
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  • Wiley-Blackwell  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Mercaptoethanol and dithiothreitol decrease the difference of electrochemical proton potentials across the yeast plasma and vacuoar membranes and activate their H+-ATPases (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 8 (1992), S. 589-598 
    Details
    ISSN: 0749-503X
    Keywords: Mercaptoethanol ; dithiothreitol ; plasmalemma ; tonoplast ; H+-ATPase ; H+-permeability ; yeast ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Mercaptoethanol and dithiothreitol (DTT) inhibited the acidification of external medium by by Saccharomyces Carlsbergensis cells and protoplasts during glucose oxidation. The inhibition was also observed when cells were incubated with mercaptoethanol or when mercaptoethanol and DTT were used to prepare protolasts. Experiments with S. carlsbergensis plasma membrene vesicles and vacuoles showed these thiol reagents to inhibitATP-dipendent generation of ΔpH and Em across plasma membrane vesicles and vacuoles but to activate their H+-ATPases. Mercaptoethanol and DTT are suggested to de-energize plasmalemma as well as tonoplast by increasing their H+-permeability and to disturb the cell ion homeostasis.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320080803
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  • 2
    Electronic Resource
    Electronic Resource
    Transport of organic acid anions and guanosine into vacuoles of Saccharomyces pastorianus (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 7 (1991), S. 495-501 
    Details
    ISSN: 0749-503X
    Keywords: Yeast ; vacuole ; H+-ATPase ; guanosine ; organic acids transport ; Ca2+-modulation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Isolated vacuoles of the yeast Saccharomyces pastorianus accumulate citrate, α-ketoglutarate, malate and guanosine. This accumulation is Mg ATP-dependent and inhibited by protonophores. The ionophores monensin and A23187 (electroneutral Men+/nH+-exchange) inhibit guanosine accumulation but fail to block citrate uptake. Mg2+ ions (2 mM) increase the values of both Δ\documentclass{article}\pagestyle{empty}\begin{document}$ \tilde \mu $\end{document}H+ components and stimulate the uptake of all the above compounds. Ca2+ ions (1 mM), hyperpolarizing the yeast vacuolar membrane and dissipating the pH gradient, inhibit guanosine uptake and stimulate that of citrate. It is concluded that guanosine is transported into yeast vacuoles by an H+/guanosine antiporter while citrate, malate and α-ketoglutarate are translocated by a uniporter(s) at the expense of the membrane potential (positive inside).
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320070509
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    Paper (German National Licenses)
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