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  • 1
    Electronic Resource
    Electronic Resource
    Pyrimidine base and ribonucleoside utilization by thePseudomonas alcaligenes group (1991)
    Springer
    Antonie van Leeuwenhoek 59 (1991), S. 263-268 
    Details
    ISSN: 1572-9699
    Keywords: cytosine deaminase ; dihydropyrimidinase ; dihydropyrimidine dehydrogenase ; nucleoside hydrolase ; Pseudomonas ; pyrimidines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pyrimidine base and ribonucleoside utilization was investigated in the two type strains of thePseudomonas alcaligenes group. As sole sources of nitrogen, the pyrimidine bases uracil, thymine and cytosine as well as the dihydropyrimidine bases dihydrouracil and dihydrothymine supported the growth ofPseudomonas pseudoalcaligenes ATCC 17440 but neither these bases nor pyrimidine nucleosides supportedPseudomonas alcaligenes ATCC 14909 growth. Ribose, deoxyribose, pyrimidine and dihydropyrimidine bases as well as pyrimidine nucleosides failed to be utilized by eitherP. pseudoalcaligenes orP. alcaligenes as sole carbon sources. The activities of the pyrimidine salvage enzymes nucleoside hydrolase, cytosine deaminase, dihydropyrimidine dehydrogenase and dihydropyrimidinase were detected in cell-free extracts ofP. pseudoalcaligenes andP. alcaligenes. InP. pseudoalcaligenes, the levels of cytosine deaminase, dihydropyrimidine dehydrogenase and dihydropyrimidinase could be affected by the nitrogen source present in the culture medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583679
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  • 2
    Electronic Resource
    Electronic Resource
    Pyrimidine ribonucleoside catabolic enzyme activities ofPseudomonas pickettii (1994)
    Springer
    Antonie van Leeuwenhoek 66 (1994), S. 307-312 
    Details
    ISSN: 1572-9699
    Keywords: pyrimidine ; nucleoside hydrolase ; cytosine deaminase ; dihydropyrimidine dehydrogenase ; dihydropyrimidinase ; phosphatase ; Pseudomonas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pyrimidine ribonucleoside catabolic enzyme activities of the opportunistic pathogenPseudomonas pickettii were examined. Of the pyrimidine and related compounds tested, only dihydrouracil (nitrogen source) and ribose (carbon source) supported growth. Thin-layer chromatographic separation of the uridine and cytidine catabolities produced byP. pickettii extracts indicated that this pseudomonad contained nucleoside hydrolase activity. Its presence was confirmed by enzyme assay. Hydrolase activity was elevated in both glucose- and ribose-grown cells relative to succinate-grown cells. Nucleoside hydrolase activity was depressed when dihydrouracil served as a nitrogen source. Cytosine deaminase activity was present in extracts prepared from succinate-, glucose- or ribose-grown cells when (NH4)2SO4 served as the nitrogen source although cells grown on glucose or ribose exhibited a higher enzyme activity. Cytosine deaminase activity was not detected in extracts prepared from cells grown on dihydrouracil as a nitrogen source. Both dihydropyrimidine dehydrogenase and dihydropyrimidinase activities were measurable inP. pickettii. The dehydrogenase activity was higher with NADH than with NADPH as its nicotinamide cofactor when uracil served as its substrate. Carbon source did not affect dehydrogenase or dihydropyrimidinase activity greatly but both activities were diminished in cells grown on the nitrogen source dihydrouracil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00882765
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