ISSN:
1573-4986
Keywords:
N-Acetylgalactosamine
;
lectins
;
dansyl derivatives
;
spectrofluorimetry
;
association constants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The synthesis of the methyl α- and β-N-dansyl-d-galactosaminides is described using methyl α,β-2-azido-2-deoxy-d-galactopyranoside as starting material. This was reduced to the corresponding methyl α,β-2-amino-2-deoxy-d-galactopyranoside and then treated with dansyl chloride to yield a mixture of methyl α,β-N-dansyl-d-galactosaminides which was separated into individual anomeric forms by flash chromatography on silica gel. Methyl α-N-dansyl-d-galactosaminide was used as a fluorescent indicator ligand in continuous substitution titrations to determine the association constants of nonchromophoric carbohydrates with theN-acetyl-d-galactosamine specific lectin fromErythrina corallodendron.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731133
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