ISSN:
1573-6822
Keywords:
cytochrome P450
;
in vitro modulation of cytochrome P450 activity
;
pulmonary xenobiotic metabolism
;
type II pneumocytes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The respiratory tract is a portal of entry for many environmental chemicals. The respiratory tract plays an important role in the detoxification or metabolic activation of these chemicals, e.g., via cytochrome P450 enzymes. Alterations in the capabilities of these enzymes to metabolize inhaled compounds can, therefore, affect the toxicity of the chemicals. The pulmonary cytochrome P450 activity has been studied in many species, but relatively little is known about this activity in the human lung tissue. In this limited study, we have investigated the possibility of modulating in vitro the P450 activity in lung slices from hamsters and humans. The alkoxyresorufin-O-dealkylase activity was measured in the S9 fraction of lung slices incubated for 24 h with 106 mol/L 20-methylcholanthrene (3MC) or β-naphthoflavone (βN). The ethoxyresorufin-O-deethylase (EROD) activity was increased by 3MC and βN in lung slices of both species. The benzyloxyresorufin-O-deethylase (BROD) activity was decreased after incubation with 3MC but increased with βN. These data show that in vitro modulation in lung slices is feasible, although technical improvement is still needed, particularly in relation to the viability of the slices.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007362329736
Permalink
