ISSN:
1573-4919
Keywords:
EGF-receptor gene
;
gene expression
;
cycloheximide
;
dexamethasone and A431 carcinoma cell line
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract Human A431 carcinoma cell line is known to have 30 fold amplified epidermal growth factor receptor (EGF-R) gene. We have studied the effect of steroid hormone dexamethasone (DEX) and protein synthesis inhibitor cycloheximide (CHX) on the expression of EGF-R gene in this cell line. DEX treatment and protein synthesis inhibition by CHX treatment cause a rapid 3 to 4 fold increase in the level of EGF-R mRNA and combined treatment of the above two agents have less than additive effect. It appears that mRNA for EGF-R accumulate within the cell during protein synthesis inhibition and upon removal of CHX, gets translated into EGF-R specific protein as judged by immuno-dot assay. We did not observe the phenomenon of ‘super induction’ nor much of an additive effect under condition of combined DEX and CHX treatment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00227481
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