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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    BioControl 45 (2000), S. 355-371 
    ISSN: 1573-8248
    Keywords: culture media ; fungal antagonism ; plant growth promotion/inhibition ; proteolytic activity ; rhizosphere-isolated Pseudomonas spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The type of culture media significantly influenced thegrowth-promoting or growth-inhibiting effect of sevenPseudomonas spp. isolates on cucumber, as didthe type of bacterial isolate. All the bacterialisolates also inhibited fungal growth, in vitroand the degree of inhibition depended on the growthmedium of the two cultures. The degree of inhibitionwas affected by the growth-rate of the fungalpathogen. Proteolytic activity was detected in thesupernatants of the cultures of six out of sevenbacterial isolates and was media-dependent. Highproteolytic activity was measured in supernatants ofbacteria grown in two organic-based media, Tryptic SoyBroth and SNB nutrient broth, and/or in a Mineralmedium. In contrast, proteolytic activity was almost undetectable after growth in King's B medium. Proteolytic activity was significantly increased when the concentration ofproteose peptone in King's B medium was reduced by 50and 75 percent.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1939
    Keywords: VAM fungi ; Benomyl ; Clipping ; Carbon allocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We examined how combinations of parentage, fungicide application, and artificial herbivory influence growth and shoot phosphorus content in pre-reproductive Lotus corniculatus, using young offspring arising from three parental crosses, two of which had one parent in common. Soil with vesicular-arbuscular mycorrhizal (VAM) fungi was treated with either water or benomyl, an anti-VAM fungicide, and added to trays containing groups of four full siblings. There were two experiments; in the first no plants were clipped while in the second two of the four plants were clipped to simulate herbivory. In both experiments plants of the two related crosses accumulated more biomass and total shoot P than did plants of the third cross. Plants inoculated with watertreated soil had greater shoot mass and P concentration than did fungicide-treated replicates but the extent of increase in P concentration varied among crosses. In Experiment 2, clipping reduced root mass and resulted in higher shoot P concentration. In this experiment there was a significant interaction of fungicide application and clipping: both unclipped and clipped plants grew better in soil not treated with fungicide, but the increase in shoot mass, total mass, and total P was greater in unclipped plants. Significant interaction of fungicide treatment and clipping is most likely due to reduced availability of carbon to the roots of clipped plants, resulting in poorer symbiotic functioning.
    Type of Medium: Electronic Resource
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